We narrowed to 4,436 results for: BRE

1. pAAV-BRX-eGFP-NLS

   Plasmid

   #217534

   Purpose

   AAV vector to drive the expression of eGFP under the control of the 4xBRE regulatory element of SMAD1 and miniXon splicing casette transcription factor in vivo

   Depositor

   Peter Scheiffele

   Article

   Okur et al bioRxiv 2023

   Insert

   BMP reporter element and miniXon casette (Smad1 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   BMP Reporter Element

   Available since

   April 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV6-AC 4-μHOM

   Plasmid

   #113619

   Purpose

   Reporter for Alt-EJ, variant of EJ7-GFP. Double strand breaks are induced with the 7a & 7h, or 7a & 7i sgRNAs, with CAS9. EJ using 4 nt of microhomology causes a deletion mutation and restores GFP

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   4-μHOM variant of EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV6-AC EJ7-GFP

   Plasmid

   #113617

   Purpose

   Reporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFP

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   EJ7-GFP variant of EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. VinTS-tCRMod-GGSGGS9

   Plasmid

   #111765

   Purpose

   Expresses vinculin tension sensor comprised of Clover-(GGSGGS)9-mRuby2 module

   Depositor

   Brenton Hoffman

   Article

   LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

   Insert

   Clover-mRuby2-based vinculin tension sensor with (GGSGGS)9 extensible domain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP VAMP3

   Plasmid

   #42310

   Depositor

   Thierry Galli

   Article

   Galli et al Mol Biol Cell. 1998 Jun;9(6):1437-48.

   Insert

   VAMP 3 (VAMP3 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. hNICD3(3xFLAG)-pCDF1-MCS2-EF1-copGFP

   Plasmid

   #40640

   Depositor

   Brenda Lilly

   Article

   Zhao et al J Biol Chem. 2012 May 11;287(20):16111-2

   Insert

   NICD3 (NOTCH3 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSC-humanADAR1

   Plasmid

   #198235

   Purpose

   Expresses human ADAR1 in yeast

   Depositor

   Brenda Bass

   Article

   Eggington et al Nat Commun. 2011;2:319. doi: 10.1038/nco

   Insert

   human ADAR1 (ADAR Human)
   

   Use

   Tags

   His10_TEV

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET51b(+)-NADPH-LUCID-3 (C50 1.4 uM)

   Plasmid

   #117910

   Purpose

   Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 1.4 uM

   Depositor

   Kai Johnsson

   Article

   Yu et al Science. 2018 Sep 14;361(6407):1122-1126

   Insert

   SNAP_Pro30_[cpNLuc23_24]eDHFR(R44A)
   

   Use

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Mutation

   R44A on eDHFR

   Promoter

   T7

   Available since

   Oct. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmiHiFiCas9

   Plasmid

   #164832

   Purpose

   miHiFiCas9 expressing plasmid

   Depositor

   Eugene Chen

   Article

   Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

   Insert

   miHiFiCas9 (contains Brex27 at sequence 5031-5138).
   

   Use

   CRISPR

   Tags

   FLAG and NLS

   Expression

   Mammalian

   Mutation

   change R 691 to A

   Promoter

   CMV

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmiCas9

    Plasmid

    #162758

    Purpose

    express miCas9

    Depositor

    Eugene Chen

    Article

    Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

    Insert

    miCas9
    

    Use

    CRISPR

    Tags

    FLAG and NLS

    Expression

    Mammalian

    Mutation

    Contains Brex27 sequence range is 4958-5065

    Promoter

    CMV

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-IRES-Gαi*-BERKY3

    Plasmid

    #158424

    Purpose

    BRET biosensor for detection of Gαi-GTP in lentiviral vector pLVX-IRES-Hyg

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
    

    Use

    Lentiviral

    Tags

    myc

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. Actin Binding Domain Tensionless Control

    Plasmid

    #215371

    Purpose

    Expresses Actin Binding Domain Tensionless Control (ABDTL), composed of Ftractin-Linker-TSMod. This is the unloaded control for Actin Binding Domain Tension Sensor (ABDTS).

    Depositor

    Brenton Hoffman

    Article

    Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    Actin Binding Domain Tensionless Control
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSc-humanADAR2

    Plasmid

    #171106

    Purpose

    Expresses human ADAR2 in yeast.

    Depositor

    Brenda Bass

    Article

    Macbeth et al Methods Enzymol. 2007;424:319-31. doi: 1

    Insert

    human ADAR2 (ADARB1 Human)
    

    Use

    Tags

    His12_TEV

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3 Flag TSC2 (S939A/T1462A)

    Plasmid

    #14131

    Depositor

    Brendan Manning

    Article

    Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

    Insert

    TSC2 S939A/T1462A (TSC2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S939A/T1462A

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

    Plasmid

    #134661

    Purpose

    Expresses EGFP-tagged synaptic vesicle protein

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    vesicle-associated membrane protein 2 (VAMP2 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 Flag TSC2 (S939A)

    Plasmid

    #14132

    Depositor

    Brendan Manning

    Article

    Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

    Insert

    TSC2 S939A (TSC2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S939A.

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-ALK7 T194D

    Plasmid

    #80886

    Purpose

    mammalian expression of ALK7 T194D

    Depositor

    Aristidis Moustakas

    Article

    Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

    Insert

    ALK7 (Acvr1c Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T194D (constitutively active)

    Promoter

    CMV

    Available since

    Aug. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pFsynW SYB2 reporter

    Plasmid

    #197276

    Purpose

    Encodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYB2 and HaloTag (Vamp2 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    N/A

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-5X1 Ash2L full

    Plasmid

    #15549

    Depositor

    Kai Ge

    Article

    Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

    Insert

    Ash2L (ASH2L Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3 Flag TSC2 (T1462A)

    Plasmid

    #14130

    Depositor

    Brendan Manning

    Article

    Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

    Insert

    TSC2 T1462A (TSC2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T1462A

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only