We narrowed to 10,873 results for: kars;

1. p53-(1-393)-R175H-N268D

   Plasmid

   #35086

   Depositor

   Cheryl Arrowsmith

   Article

   Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains aa 1-393 - R175H-N268D

   Promoter

   T7

   Available since

   April 4, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pMM9-MAPKK2-KW71 [delta(48-55)/S222D/S226D]

   Plasmid

   #40806

   Depositor

   Natalie Ahn

   Article

   Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

   Insert

   MAP2K2 (MAP2K2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta(48-55) + S222D + S226D

   Promoter

   MSV-LTR

   Available since

   Nov. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

3. DCT.VV.Orf107

   Plasmid

   #12795

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf107
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pDF0163 GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon

   Plasmid

   #172504

   Purpose

   Encodes the GwCas7x3 protein only for bacterial recombinant protein expression for purification

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)

   Plasmid

   #79619

   Purpose

   Expresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   3xFlag-PmCDA1

   Expression

   Mammalian

   Mutation

   D10A for nickase Cas9

   Promoter

   pCMV

   Available since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSMP-Bmi1_2

   Plasmid

   #36353

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   Bmi1 (BMI1 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pBMN_HA-TBK1 (E696K)

   Plasmid

   #199207

   Purpose

   Used for the expression of TBK1 carrying E696K mutation (SMC Internal No. 1998)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   E696K

   Promoter

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFUW-TetO-SUB1

   Plasmid

   #178440

   Purpose

   doxycycline-inducible expression of Human SUB1 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

   Insert

   SUB1 (SUB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1_pCMV-dCas-PmCDA1 pH1-gRNA(HPRT)

   Plasmid

   #79621

   Purpose

   Expresses dCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Mammalian

   Mutation

   D10A and H840A for nuclease deficient Cas9

   Promoter

   pCMV

   Available since

   Sept. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EGFR∆550-580-mEGFP

    Plasmid

    #203788

    Purpose

    Number and brightness analysis of EGFR oligomerization

    Depositor

    Thorsten Wohland

    Article

    Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

    Insert

    EGFR (EGFR Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    aa 550-580 deleted

    Promoter

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSMP-SETDB1_1

    Plasmid

    #36347

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    SETDB1 (SETDB1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pMKO.1 puro-luc (shRNA)

    Plasmid

    #153962

    Purpose

    Expresses a shRNA against firefly luciferase

    Depositor

    Hiroyuki Konishi

    Article

    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

    Insert

    shRNA against firefly luciferase (19-bp-long target)
    

    Use

    Luciferase, RNAi, and Retroviral

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HP21-TPI-PTC1

    Plasmid

    #131043

    Purpose

    Expresses TPI-PTC1 NMD reporter (It has a Premature termination codon (PTC) at First Amino acid) with a 21 nt hairpin in 5'UTR

    Depositor

    Marvin Tanenbaum

    Article

    Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

    Insert

    TPI-PTC1 (TPI1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PTC1

    Promoter

    Pcmv

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBS-Nvec-codonopti-Cas9-2xNLS

    Plasmid

    #141108

    Purpose

    Plasmid containing insert for the IVT of nuclear localized Cas9 mRNA with codon optimization for Nematostella vectensis

    Depositor

    Matthew Gibson

    Article

    Hill et al Methods Mol Biol. 2022;2450:437-465. doi

    Insert

    Nematostella codon optimized Cas9
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    All amino acids coded for by codons optimized for…

    Promoter

    T7

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PABPN1L

    Plasmid

    #155807

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    PABPN1L (PABPN1L Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. DCT.VV.Orf117

    Plasmid

    #12805

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf117
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

17. MigR1 Fli-1 GFP

    Plasmid

    #24668

    Depositor

    Yaacov Ben-David

    Article

    Cui et al Blood. 2007 Oct 1. 110(7):2631-40.

    Insert

    Fli-1 (Fli1 Mouse)
    

    Use

    Retroviral

    Tags

    GFP and IRES

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pLNHA-C1-HsTTP_1-313_AH

    Plasmid

    #148879

    Purpose

    Mammalian Expression of HsTTP_1-313

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

    Insert

    HsTTP_1-313 (ZFP36 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSLQ7780 mU6:- || CMV: mCherry~P2A-DD-AcrIIA4

    Plasmid

    #123658

    Purpose

    DD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control without sgRNA following mU6 promoter.

    Depositor

    Stanley Qi

    Article

    Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. DCT.VV.Orf42

    Plasmid

    #12730

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf42
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2006

    Availability

    Academic Institutions and Nonprofits only