We narrowed to 5,312 results for: pcDNA3.1(+)

1. pcDNA-mCherry-4A/4B-CAAX

   Plasmid

   #87699

   Purpose

   Express mCherry with a peptide linker (contaning a HCVp 4A/4B substrate site) and a C-terminal CAAX box from KRras for plasma-membrane targeting in mammalian cells

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   mCherry-4A/4B-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. SPLICS Po-ER Short P2A

   Plasmid

   #164114

   Purpose

   Detect the long Peroxisomes-Endoplasmic reticulum contact

   Depositor

   Tito Calì, Marisa Brini

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split YFP Po-ER Short P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTOPO-MCL1

   Plasmid

   #21605

   Depositor

   Ulrich Maurer

   Article

   Maurer et al Mol Cell. 2006 Mar 17. 21(6):749-60.

   Insert

   MCL-1 (MCL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Insert ends with a STOP codon, no V5-His-tag.

   Promoter

   Available Since

   Aug. 11, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-FKBP-ICUE3-NLS

   Plasmid

   #138255

   Purpose

   FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.

   Depositor

   Jin Zhang

   Article

   Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

   Insert

   FKBP-ICUE3-NLS
   

   Use

   Tags

   FKBP, Nuclear localization signal (NLS), cpVenus[…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAB1679 CMV-HIVNES-GS-Cas13bt3

   Plasmid

   #176317

   Purpose

   CMV-HIVNES-GS-Cas13bt3 (human codon optimized Cas13bt3 expression)

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

   Insert

   human codon optimized Cas13bt3
   

   Use

   CRISPR

   Tags

   HIV NES

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA -CLCN3-3xFlag

   Plasmid

   #225506

   Purpose

   CLCN3 expression vector

   Depositor

   Wade Harper

   Article

   Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

   Insert

   CLCN3 (CLCN3 Human)
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-FLAG-ATBF1

   Plasmid

   #40927

   Depositor

   Jin-Tang Dong

   Article

   Dong et al J Biol Chem. 2010 Oct 22;285(43):32801-9

   Insert

   ATBF1 (ZFHX3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   See depositor comments below.

   Promoter

   CMV

   Available Since

   March 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. cyt-GafD-mTurboID-V5

   Plasmid

   #184640

   Purpose

   Expresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome tagging

   Depositor

   Charlie Fehl

   Article

   Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021

   Insert

   cyt-GlycoID-V5
   

   Use

   Tags

   Nuclear export sequence and V5 tag

   Expression

   Mammalian

   Mutation

   GafD -> expressed aa22-178 ; BirA* -> expre…

   Promoter

   CMV

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. 3NES-CS(G)-tTA-CS(G)-3NES

   Plasmid

   #137836

   Purpose

   Soluble tTA transcription factor flanked by N and C terminal TEV protease G cleavage sequences and 3 nuclear export sequences

   Depositor

   Joshua Leonard

   Article

   Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

   Insert

   3NES-CD(G)-tTA-CS(G)-3NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HA-MCPIP1

    Plasmid

    #127459

    Purpose

    Expressing human MCPIP1 in mammalian cells

    Depositor

    Mingui Fu

    Article

    Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

    Insert

    MCPIP1 (ZC3H12A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-HCMVgL-FLAG (TB40/E)

    Plasmid

    #136449

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein L (TB40/E strain) with FLAG tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein L (UL115 Human betaherpesvirus 5)
    

    Use

    Tags

    Short SGGSG linker and FLAG tag

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3-Flag-Tpr

    Plasmid

    #60882

    Purpose

    Expresses Flag-Tagged nucleoporin Tpr in Mammalian Cells

    Depositor

    Vinay Nandicoori

    Article

    Vomastek et al Mol Cell Biol. 2008 Nov;28(22):6954-66.

    Insert

    Tpr (TPR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. nuc-GafD-mTurboID-HA

    Plasmid

    #184641

    Purpose

    Expresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome tagging

    Depositor

    Charlie Fehl

    Article

    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021

    Insert

    nuc-GlycoID-HA
    

    Use

    Tags

    3xHA tag and Nuclear localization sequence

    Expression

    Mammalian

    Mutation

    GafD -> expressed 22-178 ; BirA* -> express…

    Promoter

    CMV

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-HCMVgH-8his (Merlin)

    Plasmid

    #136447

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain) extracellular domain with 8his purification tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein H (UL75 Human betaherpesvirus 5)
    

    Use

    Tags

    Short GGSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular domain (a.a. 1-716). Codon-optimize…

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA-3xFLAG-NLS-TPP1

    Plasmid

    #53585

    Purpose

    Mammalian expression of 3xFLAG tagged, nuclear localized TPP1, N-terminal.

    Depositor

    Steven Artandi

    Article

    Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

    Insert

    TPP1 (ACD Human)
    

    Use

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Silent mutation to eliminate PacI site

    Promoter

    CMV

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA_RGG-GFP-RGG_withTEVsite

    Plasmid

    #124939

    Purpose

    Expresion of RGG_GFP-RGG

    Depositor

    Matthew Good, Benjamin Schuster, Daniel Hammer

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    TEV site between RGG and EGFP

    Promoter

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. mVenus-IQ6 R6A

    Plasmid

    #198202

    Purpose

    FRET acceptor: Mutated IQ6 domain of Moysin Va tagged with N-terminal mVenus (lower binding affinity)

    Depositor

    Christian Wahl-Schott

    Article

    Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

    Insert

    MYO5A IQ domain (Myo5a Mouse)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    R6A

    Promoter

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC3.1_CMV_oROS-G

    Plasmid

    #216111

    Purpose

    Expresses the genetically encoded green fluorescent hydrogen peroxide sensor in mamalian cells.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.01.31.578117

    Insert

    oROS-G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA SF-HERC2 F2

    Plasmid

    #55785

    Purpose

    Expresses N-terminal 3xFLAG/STREP tagged HERC2 (950-1750aa)

    Depositor

    David Chan

    Article

    Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

    Insert

    3xFLAG tagged HERC2 (950aa-1750aa) (HERC2 Human)
    

    Use

    Tags

    3xFLAG and STREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

20. FLAG HDAC5 WT

    Plasmid

    #32213

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    HDAC5 (HDAC5 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 20, 2012

    Availability

    Academic Institutions and Nonprofits only