164,884 results

1. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV9)

   Viral Prep

   #20298-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin H134R mutant fused to EYFP, for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   EF1a

   Tags

   EYFP (Cre-dependent)

   Available since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

2. KRAB-dSpCas9

   Plasmid

   #205248

   Purpose

   In vitro transcription plasmid for production of KOX1 KRAB-dSpCas9 mRNA for transcriptional repression (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #85449.

   Depositor

   Rasmus Bak

   Article

   Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

   Insert

   KRAB-dSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. drebrin-YFP

   Plasmid

   #40359

   Depositor

   Phillip Gordon-Weeks

   Article

   Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

   Insert

   drebrin E2 (DBN1 Human)
   

   Use

   Chick expression

   Tags

   eYFP

   Expression

   Mutation

   Promoter

   chick B-actin promoter with CMV enhancer

   Available since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-DIO-hM4D(Gi)-mCherry

   Plasmid

   #44362

   Purpose

   Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.

   Depositor

   Bryan Roth

   Article

   Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   hM4D(Gi)-mCherry
   

   Use

   AAV; Adeno associated viral vector

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available since

   April 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C1 Lifeact-EGFP

   Plasmid

   #58470

   Purpose

   Expresses a cytoplasmic actin filament reporter, the peptide Lifeact, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Bioarchitecture. 2015 Aug 28:1-14.

   Insert

   Lifeact
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Mouse sgRNA library Brie in lentiGuide-Puro

   Pooled Library

   #73633

   Purpose

   Mouse sgRNA library in backbone lentiGuide-Puro containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Has Service

   Lentiviral Prep

   Expression

   Mammalian

   Use

   CRISPR and Lentiviral

   Available since

   Feb. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pAAV2/rh10

   Plasmid

   #112866

   Purpose

   AAV packaging plasmid expressing Rep/Cap genes

   Depositor

   James M. Wilson

   Insert

   Rep2/Cap-rh10
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Truncated P5

   Available since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-DIO-mCherry (AAV2)

   Viral Prep

   #50459-AAV2

   Purpose

   Ready-to-use AAV2 particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Promoter

   Syn

   Tags

   mCherry (Cre-dependent)

   Available since

   Nov. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV2)

   Viral Prep

   #44361-AAV2

   Purpose

   Ready-to-use AAV2 particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Article

   Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

   Promoter

   Syn

   Tags

   mCherry (Cre-dependent)

   Available since

   Nov. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

10. XLone-GFP

    Plasmid

    #96930

    Purpose

    Tunable and temporal expression control of GFP

    Depositor

    Xiaojun Lian

    Article

    Randolph et al Sci Rep. 2017 May 8;7(1):1549. doi: 10.1

    Insert

    Green Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS

    Available since

    Aug. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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