We narrowed to 4,454 results for: TIL

1. pSUMO1

   Plasmid

   #52258

   Purpose

   Bacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Inserts

   SUMO-1 (SUMO1 Human)
   UBE2l (UBE2I Human)
   SAE-2 (UBA2 )
   SAE-1 (SAE1 Human)
   

   Use

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   C-terminal gly-gly

   Promoter

   T7 and rbs

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

   Plasmid

   #105842

   Purpose

   Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment

   Depositor

   Michael Ward

   Article

   Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

   Inserts

   Neurogenin 2 (NEUROG2 Human)
   ISL LIM Homeobox 1 (ISL1 Human)
   LIM Homeobox 3 (LHX3 Human)
   Optimized mApple
   rtTA3G
   SBP Delta-LNGFR
   

   Use

   CRISPR and TALEN

   Tags

   Expression

   Mutation

   Promoter

   CAG, EF-1alpha, and TRE3G

   Available since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-SARS2SΔC-H2gp41

   Plasmid

   #170389

   Purpose

   Generation of SARS-CoV-2 spike pseudotyped lentivirus

   Depositor

   Nir Hacohen

   Article

   Filbin et al Cell Rep Med. 2021 May 18;2(5):100287. d

   Insert

   SARS-CoV-2 Spike (S SARS-CoV-2)
   

   Use

   Tags

   HIV gp41

   Expression

   Mammalian

   Mutation

   Deletion of C terminal and fusion with H2 from HI…

   Promoter

   CMV

   Available since

   June 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pcDNA3.1_ spike_del19

   Plasmid

   #155297

   Purpose

   Expression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particles

   Depositor

   Raffaele De Francesco

   Article

   Conforti et al Mol Ther. 2021 Sep 20. pii: S1525-0016(2

   Insert

   SARS-Cov2 spike_deleted (S SARS-Cov2)
   

   Use

   Generation of sars-cov-2-pseudotyped lentiviral p…

   Tags

   Expression

   Mutation

   Deletion in C-term 19 aa of spike

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pTRIP-SFFV-Hygro-2A-TMRPSS2

   Plasmid

   #170390

   Purpose

   Generation of stable lines to enhance SARS-CoV-2 infectivity in ACE2 expressing cells

   Depositor

   Nir Hacohen

   Article

   Filbin et al Cell Rep Med. 2021 May 18;2(5):100287. d

   Insert

   TMPRSS2 (TMPRSS2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   June 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. MG1655 Z1 malE

   Bacterial Strain

   #65915

   Purpose

   E. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE KO

   Depositor

   Keith Tyo

   Article

   Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

   Bacterial Resistance

   None

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

7. MG1655 Z1 malE clpA

   Bacterial Strain

   #65918

   Purpose

   E. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE, clpA KO

   Depositor

   Keith Tyo

   Article

   Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

   Bacterial Resistance

   None

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

8. MG1655 Z1 malE aat

   Bacterial Strain

   #65917

   Purpose

   E. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE, aat KO

   Depositor

   Keith Tyo

   Article

   Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

   Bacterial Resistance

   None

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

9. RHO3

   Bacterial Strain

   #124700

   Purpose

   E. coli mobilizer strain that facilitates conjugation of mobilizable plasmids by using metabolic counter-selection against the donor strain.

   Depositor

   Herbert Schweizer

   Article

   Lopez et al Appl Environ Microbiol. 2009 Oct;75(20):

   Bacterial Resistance

   Dap (400 µg/ml)

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

10. B95(DE3) ΔA ΔfabR ΔserB

    Bacterial Strain

    #197655

    Purpose

    This strain is used for expressing phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein.

    Depositor

    Ryan Mehl, Richard Cooley

    Article

    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

    Bacterial Resistance

    None

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

11. E. coli MEV20

    Bacterial Strain

    #197113

    Purpose

    E. coli BL21(DE3) derivative for diterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Bacterial Resistance

    None

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

12. pETDest

    Plasmid

    #124245

    Purpose

    Allows Gateway cloning into the bacterial 6xHIS tagged protein expression vector pET26

    Depositor

    Attila Fehér

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDOE20 mC VN

    Plasmid

    #124246

    Purpose

    Allows fluorescent (mVENUS; mCherry) co-localization of two proteins in Agrobacterium- transformed plant cells.

    Depositor

    Attila Fehér

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28b_NOSER

    Plasmid

    #124244

    Purpose

    Bacterial 6xHIS tagged protein expression without the addition of any potential phosphorylation sites to the tagged protein.

    Depositor

    Attila Fehér

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDOE20 mC VN transient

    Plasmid

    #124247

    Purpose

    Allows fluorescent (mVENUS; mCherry) co-localization of two proteins in directly-transfected plant cells.

    Depositor

    Attila Fehér

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pwA-ple34

    Plasmid

    #227337

    Purpose

    Heterologous production of pleuromutilin in Aspergillus oryzae

    Depositor

    Jun-ichi Maruyama

    Insert

    The upstream/inside regions of the wA gene and expression cassettes of the ple3 and ple4 genes
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. hOlig1-myc/pCDNA3.1

    Plasmid

    #197337

    Purpose

    Mammalian expression of an epitope-tagged hOLIG1

    Depositor

    Charles Stiles

    Insert

    OLIG1(pSM1-3) (OLIG1 Human)
    

    Use

    Tags

    Myc-his

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. ppG-ple12

    Plasmid

    #227339

    Purpose

    Heterologous production of pleuromutilin in Aspergillus oryzae

    Depositor

    Jun-ichi Maruyama

    Insert

    The upstream/downstream regions of the pyrG gene and expression cassettes of the ple1 and ple2 genes
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLSU1/t35s[]:mCherry:NosT

    Plasmid

    #212177

    Purpose

    This binary vector expresses mCherry with the truncated 35sCAMV promoter and no UTR

    Depositor

    Wayne Curtis

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Truncated mCherry, missing the first 9 amino acid…

    Promoter

    truncated 35sCaMV

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pANY4

    Plasmid

    #164897

    Purpose

    Two valuable constructs for either IPTG-induced or temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4.

    Depositor

    Yingfeng An

    Insert

    ccdB cassette
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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