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We narrowed to 63,812 results for: cloning

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Showing: 4021 - 4040 of 63812 results

  4. gRNA_DNMT3a-T2

    Plasmid
    #41822
    Purpose
    Expresses a guide RNA (gRNA) to target DNMT3a (T2 target sequence) for genome engineering
    Depositor
    George Church
    Publication
    Mali et al Science. 2013 Feb 15;339(6121):823-6. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA_DNMT3a-T2 (DNMT3A Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Jan. 11, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. pEZY19

    Plasmid
    #18668
    Purpose
    pET19b converted Gateway Destination vector for expressing His tagged fusion proteins in bacteria. See note in comments section below regarding the mutated enterokinase site.
    Depositor
    Yu-Zhu Zhang
    Publication
    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.
    Type
    Empty backbone
    Use
    Gateway Cloning
    Tags
    His
    Expression
    Bacterial
    Promoter
    T7
    Available since
    July 26, 2008
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    18668-plasmid-map-sequence-id-8812

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  18. Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)

    Plasmid
    #208942
    Purpose
    The CE1 construct, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    208942-plasmid-map-sequence-id-415667

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  20. pM-Cas12a

    Plasmid
    #196291
    Purpose
    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding LbCas12a in place of viral GP
    Depositor
    Zhenghe Li
    Publication
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV M antigenome encoding LbCas12a in place of viral GP (cas12a Synthetic)
    Use
    CRISPR
    Tags
    Flag
    Expression
    Plant
    Mutation
    See depositor comments below
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196291-plasmid-map-sequence-id-389824

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Showing: 4021 - 4040 of 63812 results