We narrowed to 7,548 results for: ski

1. pDule-IBBN (G2)

   Plasmid

   #85500

   Purpose

   Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

   Insert

   IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y32G L65E F108W Q109M D158S R162K

   Promoter

   lpp (constitutive)

   Available since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX Txk-SH2

   Plasmid

   #46526

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Txk (Txk Mouse)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 138-258

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-MBP-TEVpCS-ADGRB2 CTF-FLAG

   Plasmid

   #217682

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRB2 (BAI2 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-911

   Promoter

   Available since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHS1068

   Plasmid

   #205962

   Purpose

   NZ_CP025815 DinG HNH proteins (E. coli codon optimized) and CRISPR array in pACYCDuet-1 with Lac promoters

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   DinG HNH proteins and CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac (proteins), pJ23119 (crRNA)

   Available since

   Feb. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pInducer10-UBC9-sh4

   Plasmid

   #168988

   Purpose

   Expresses RFP cDNA and UBC9 shRNA 4 in mammalian cells

   Depositor

   Ji Luo

   Article

   Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   Ubiquitin Conjugating Enzyme 9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc promoter

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMM9-MAPKK2-KW71 [delta(48-55)/S222D/S226D]

   Plasmid

   #40806

   Depositor

   Natalie Ahn

   Article

   Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

   Insert

   MAP2K2 (MAP2K2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta(48-55) + S222D + S226D

   Promoter

   MSV-LTR

   Available since

   Nov. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pGEX Sh3bp2-SH2

   Plasmid

   #46484

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Sh3bp2 (SH3BP2 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 450-561

   Promoter

   Tac

   Available since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDF0163 GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon

   Plasmid

   #172504

   Purpose

   Encodes the GwCas7x3 protein only for bacterial recombinant protein expression for purification

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA5/FRT_CMV_NES-Caprola_06-mEGFP

   Plasmid

   #194684

   Purpose

   CMV driven expression of the calcium recorder Caprola_06 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_06-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD-R-iLACCO1.1

    Plasmid

    #208020

    Purpose

    Biosensor for intracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    R-iLACCO1.1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJL1-mRFP1 gRNA

    Plasmid

    #117052

    Purpose

    In vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

    Insert

    anti-mRFP1 SpCas9 gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3.0_mitoLAMA-G97 IRES ShadowG-mScarlet

    Plasmid

    #130709

    Purpose

    Plasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and ShadowG-mScarlet

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    NTOM20-SNAP-Tag-LAMA-G97 IRES ShadowG-mScarlet
    

    Use

    Tags

    NTOM20 and SNAP-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLQ10714

    Plasmid

    #183959

    Purpose

    U6-crKlf4-CAG-hyperdCas12a-miniVPR

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Inserts

    HyperdCas12a
    crRNA targeting Klf4
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D832A, D156R, E292R, D235R, D350R

    Promoter

    CAG and U6

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. LENTICRISPR-PGAM1_sgRNA2

    Plasmid

    #201615

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    PGAM1 (PGAM1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCIBN-EYFP

    Plasmid

    #103815

    Purpose

    Soluble BLInCR construct that can interact with PHR constructs upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN (CIB1 Mustard Weed)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCEP4-gp120(BaL)-CD4

    Plasmid

    #98957

    Purpose

    Mammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    HIV-1 (BaL) gp120
    

    Use

    Tags

    CD4 (domains D1-D2), CD5 leader sequence, FLAG ep…

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX458-sgRNA_Ago1_1

    Plasmid

    #73533

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago1

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX458-sgRNA_Ago1_2

    Plasmid

    #73534

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago1

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

19. TXD13-CD4d3+4-bio

    Plasmid

    #73108

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding TXD13 with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    TXD13 (TMX4 Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. bio-CD4d3+4-ADL1

    Plasmid

    #73090

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding ADL1 with bio-rat CD4d3+4

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    ADL1 (NCEH1 Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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