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pUG-334

Plasmid

#104759

Purpose

Level 2 construct

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

HIS-Ubq_UBA1_HA-UBC35_MMZ4_EL4

Tags

HIS-Ubq, HA-E2

Expression

Bacterial

Promoter

T7

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-338

Plasmid

#104763

Purpose

Level 2 construct

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

HIS-Ubq_UBA1_HA-UBC36_MMZ4_EL4

Tags

HIS-Ubq, HA-E2

Expression

Bacterial

Promoter

T7

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pET15b-E228A-PvuRts1I

Plasmid

#86896

Purpose

express E228A variant PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

PvuRts1I (pvuRts1I )

Tags

His

Expression

Bacterial

Mutation

E228A

Promoter

T7

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b-W215A-PvuRts1I

Plasmid

#86898

Purpose

express W215A variant PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

PvuRts1I (pvuRts1I )

Tags

His

Expression

Bacterial

Mutation

W215A

Promoter

T7

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-PvuRts1I

Plasmid

#86888

Purpose

express C-term His tagged PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

PvuRts1I (pvuRts1I )

Tags

C-term His tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b-E71A-PvuRts1I

Plasmid

#86895

Purpose

express E71A variant PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

PvuRts1I (pvuRts1I )

Tags

His

Expression

Bacterial

Mutation

E71A

Promoter

T7

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b-D57A-PvuRts1I

Plasmid

#86892

Purpose

express D57A variant PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

PvuRts1I (pvuRts1I )

Tags

His

Expression

Bacterial

Mutation

D57A

Promoter

T7

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b-R208A-PvuRts1I

Plasmid

#86891

Purpose

express R208A variant PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

PvuRts1I (pvuRts1I )

Tags

His

Expression

Bacterial

Mutation

R208A

Promoter

T7

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b-E20A-PvuRts1I

Plasmid

#86897

Purpose

express E20A variant PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

Pvurts1I (pvuRts1I )

Tags

His

Expression

Bacterial

Mutation

E20A

Promoter

T7

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b-D70A-PvuRts1I

Plasmid

#86890

Purpose

express D70A variant PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

PvuRts1I (pvuRts1I )

Tags

His

Expression

Bacterial

Mutation

D70A

Promoter

T7

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221 Tma16

Plasmid

#63779

Purpose

yeast ORF Tma16 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Tma16 (TMA16 Budding Yeast)

Use

Gateway donor vector

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pMZ031

Plasmid

#66971

Purpose

Expresses zebrafish Chk1 mutant with C-term GFP fluorescent tag

Depositor

Michael Lampson

Article

Zhang et al Cell Cycle. 2014;13(24):3828-38. doi: 10

Insert

zebrafish Chk1

Tags

GFP

Mutation

mutated S256E, S280E, T292E, and S301E

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Lck-mStayGold

Plasmid

#234539

Purpose

to label the membrane of cells with a fluorescent protein (mStayGold)

Depositor

Hajime Hirase

Article

Wang et al Anat Sci Int. 2025 Jun 27. doi: 10.1007/

Insert

Lck-mStayGold(Ando)

Use

AAV

Mutation

None

Promoter

Short CAG

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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AP1

Plasmid

#179678

Purpose

Accessory plasmids

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[SD8] gIII

Expression

Bacterial

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Lck-mScarlet3

Plasmid

#234540

Purpose

to label the membrane of cells with a fluorescent protein (mScarlet3)

Depositor

Hajime Hirase

Article

Wang et al Anat Sci Int. 2025 Jun 27. doi: 10.1007/

Insert

Lck-mScarlet3

Use

AAV

Mutation

None

Promoter

Short CAG

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Lck-mTagBFP2

Plasmid

#234537

Purpose

to label the membrane of cells with a fluorescent protein (mTagBFP2)

Depositor

Hajime Hirase

Article

Wang et al Anat Sci Int. 2025 Jun 27. doi: 10.1007/

Insert

Lck-mTagBFP2

Use

AAV

Mutation

None

Promoter

Short CAG

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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pBigTB

Plasmid

#149432

Purpose

shuttle vector with blasticidin-S resistance into ROSA26 targetting vectors

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Type

Empty backbone

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pUAST tdTomato mouse Rab35 WT

Plasmid

#53514

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Mouse)

Tags

tdtomato

Expression

Insect

Promoter

GAL4

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
mTagBFP2-Cx43-7

Plasmid

#55288

Purpose

Localization: Gap Junctions, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Cx43

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pUAST YFP mouse Rab35 WT

Plasmid

#53517

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Mouse)

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only