We narrowed to 26,744 results for: Sis;
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Plasmid#118365PurposeThis plasmid expresses human HSF1 S303A and S307A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.DepositorAvailable SinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only
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pNOC_hfnCas12a-Nlux
Plasmid#176239PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux under the control of Ribi promoterDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferasePromoterRibosomal subunitAvailable SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a_Nlux_(-HH)crRNA NR1
Plasmid#176250PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1 without the hammerhead ribozymDepositorInserthumanized fnCas12a
UseCRISPR and Synthetic Biology; Expression in micro…Available SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hlbCas12a-Nlux
Plasmid#176240PurposepNOC episomal plasmid harboring the humanized lbCas12a gene sequence tagged with Nlux under the control of Ribi promoterDepositorInserthumanized lbCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferasePromoterRibosomal subunitAvailable SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a_NR1_NR2_LPT1
Plasmid#176253PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene and one spacer targeting the LPAAT geneDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseAvailable SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAN19-3xFLAG-ccvTIR1-NOSt
Plasmid#108546PurposeCloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminatorDepositorInsertTRANSPORT INHIBITOR RESPONSE 1 [F79G] fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
UseCloning vectorTags3xFLAGMutationChanged F79 to GAvailable SinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a_NR1_NR2
Plasmid#176252PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene of N. oceanica IMET1 separated by fullDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero11
Plasmid#187928PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero11DepositorAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta3(702-1094)-GFP
Plasmid#100734PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertAP3B1 (AP3B1 Human)
TagsFKBP and GFPExpressionMammalianMutationappendage and ear onlyPromoterCMVAvailable SinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgGria1
Plasmid#124867PurposeMutagenesis of Gria1DepositorInsertGria1 (Gria1 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta2(616-951)-mRuby2
Plasmid#100730PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertAP2B1 (AP2B1 Human)
TagsFKBP and mRuby2ExpressionMammalianMutationLong isoformPromoterCMVAvailable SinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
CD8-pTagRFP657-LOVpep (T406A, T407A, I532A)
Plasmid#100742PurposeTo express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosisDepositorInsertCD8A (CD8A Human)
TagsLOVpep and tagRFP657ExpressionMammalianMutationTruncatedPromoterCMVAvailable SinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgGria3
Plasmid#124855PurposeMutagenesis of Gria3DepositorInsertGria3 (Gria3 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgBAX-2
Plasmid#210130Purposeknock out BAX in mammalian cellsDepositorInsertApoptosis regulator BAX (BAX Human)
UseCRISPRAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-HIS-ELP-ddFLN4-I27-XMod-Doc (wild type)
Plasmid#153442PurposeE. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 and I27 fingerprint domains. This construct was designed for AFM measurements.DepositorInsertRc.XDocB
Tags3xELP linker, 6xHis, Titin I27, ddFLN4 fingerprin…ExpressionBacterialPromoterT7 promoterAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero13
Plasmid#187929PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero13DepositorAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWZLblasti-HA-MEK1 C121S
Plasmid#53165PurposeExpresses HA tagged human MEK1 C121S from blasticidin resistance retroviral vectorDepositorAvailable SinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 S303A
Plasmid#118363PurposeThis plasmid expresses human HSF1 S303A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.DepositorAvailable SinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-TAP(FLAG-HA) CYLD
Plasmid#60031PurposeCYLD with N-terminal TAP-tag (HA,FLAG) and IRES-Puro to allow tagged protein to be maintainedDepositorInsertCylindromatosis (CYLD Human)
UseRetroviralTagsFLAG, HA, and IRES PuroExpressionMammalianPromoterLTR-driven expressionAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
CD8-dCherry-FRB
Plasmid#100739PurposeTo express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis (dark mCherry K70N)DepositorInsertCD8A (CD8A Human)
TagsFRB and dCherryExpressionMammalianMutationDark mCherry (K70N) non-fluorescentPromoterCMVAvailable SinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only