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pCF826-recipient_U6-sgRNA-EFS-Cas9-P2A-mCherry2

Plasmid

#211688

Purpose

U6-sgRNA-EFS-Cas9-P2A-mCherry2

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and guide RNA recipient vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CHyMErA_U6(SpCas9_I-SceI)-(AsCas12_PacI)_PGK-puro

Plasmid

#189634

Purpose

Lentiviral expression of single SpCas9 and AsCas12a gRNAs for generating combinatorial CHyMErA 3Cs libraries

Depositor

Manuel Kaulich

Article

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

hU6 Cas9-Cas12a gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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A3Bctd-L7G-Cas9n-UGI-NLS

Plasmid

#198886

Purpose

Express A3Bctd-L7G BE3-based base editor

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)

Tags

NLS

Expression

Mammalian

Mutation

Loop 7 of A3G

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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A3Bctd-D314E-Cas9n-UGI-NLS

Plasmid

#198887

Purpose

Express A3Bctd-D314E BE3-based base editor

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)

Tags

NLS

Expression

Mammalian

Mutation

D314E

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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eA3Ai-Cas9n-UGI-NLS

Plasmid

#207165

Purpose

Express A3A with an N57G point mutation and an intron in a BE3-based base editor

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3A (APOBEC3A Human)

Tags

Cas9-UGI--NLS

Expression

Mammalian

Mutation

N57G

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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eA3Ai-max

Plasmid

#207166

Purpose

Express A3A with an N57G point mutation and an intron in a BE4max-based base editor

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3A (APOBEC3A Human)

Tags

Cas9-UGI-UGI-NLS and NLS

Expression

Mammalian

Mutation

N57G

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196081

Purpose

Inducible CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196083

Purpose

Inducible CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196075

Purpose

Constitutive CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196077

Purpose

Constitutive CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUBI-RZ-Cas12j2

Plasmid

#189781

Purpose

Cas12j2 (CasΦ) Gateway gRNA entry plasmid using Zea mays Ubi promoter and HH, HDV ribozyme processing

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Insert

gRNA cloning site

Use

CRISPR

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK91 pHomL-TDH3p-Neon(gfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)

Plasmid

#179075

Purpose

Vector containing a yeast integration cassette for expressing TDH3p-Neon(gfp)-Link-SCS2tmh, an overexpressed (bright) marker for the endoplasmic reticulum

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

TDH3p-Neon(gfp)-Link-SCS2tmh

Expression

Yeast

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK66 pHomL-RPL18Bp-TOM70-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179050

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of TOM70-Link-Neon(gfp), a marker for the mitochondria

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-TOM70-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK65 pHomL-RPL18Bp-preCOX4-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179049

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of preCOX4-Link-Neon(gfp), a marker for the mitochondria

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-preCOX4-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK64 pHomL-RPL18Bp-PEX25-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179048

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PEX25-Link-Neon(gfp), a marker for the peroxisome

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PEX25-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK63 pHomL-RPL18Bp-PDR16-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179047

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PDR16-Link-Neon(gfp), a marker for the lipid droplet

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PDR16-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK62 pHomL-RPL18Bp-ERG6-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179046

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of ERG6-Link-Neon(gfp), a marker for the lipid droplet

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-ERG6-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK61 pHomL-RPL18Bp-Neon(gfp)-Link-ATG8-SSA1t-HomR (AmpR)

Plasmid

#179045

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-ATG8, a marker for the autophagosome

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Neon(gfp)-Link-ATG8

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK60 pHomL-RPL18Bp-PRC1-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179044

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PRC1-Link-Neon(gfp), a marker for the vacuole

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PRC1-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK59 pHomL-RPL18Bp-Neon(gfp)-Link-VAM3-SSA1t-HomR (AmpR)

Plasmid

#179043

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-VAM3, a marker for the vacuole

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Neon(gfp)-Link-VAM3

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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