We narrowed to 7,489 results for: addgene/1000

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pCAG-MBP-ATG9-H837A

Plasmid

#188079

Purpose

Expresses human ATG9-H837A mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

H837A

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ATG9 (1-830)

Plasmid

#188086

Purpose

Expresses human ATG9 C-terminal truncation mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (1-830)

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ATG9-P833A

Plasmid

#188083

Purpose

Expresses human ATG9-P833A mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

P833A

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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Dendra-Rab11a

Plasmid

#184038

Purpose

Expresses red to green photoconvertible Rab11a

Depositor

Heidi Hehnly

Article

Krishnan et al Life Sci Alliance. 2022 Mar 18;5(7). pii

Insert

Rab11 (RAB11A Human)

Tags

Dendra

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pKT2/CAGXSP/PalmGamillus

Plasmid

#182971

Purpose

Stably express PalmGamillus in mammalian cells via the sleeping beauty transposon system.

Depositor

Masamitsu Kanada

Article

Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

Insert

PalmGamillus

Expression

Mammalian

Mutation

N/A

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-zfKitlga

Plasmid

#168199

Purpose

Express zebrafish Kitlga, mRNA synthesis

Depositor

Petr Bartunek

Article

Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

Insert

Kitlga (kitlga Zebrafish)

Use

Vertebrate expression (zebrafish, chicken, xenopu…

Expression

Mammalian

Mutation

F198L - please see depositor comments

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnj8

Plasmid

#173134

Purpose

This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

Insert

kcnj8 (kcnj8, si:dkey-183c2.2 Zebrafish)

Use

Pcr cloning vector

Promoter

No promoter at 5 end; T7 promoter at 3 end.

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pETM10 with rat Δ192Annexin A11

Plasmid

#164498

Purpose

For bacterial expression of rat His-tagged Δ192Annexin A11

Depositor

Anni Vedeler

Article

Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

Insert

rat Δ192Annexin A11 (Anxa11 Rat)

Tags

His

Expression

Bacterial

Mutation

N-terminal truncation

Promoter

T7

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pETM10 with rat Δ188Annexin A11

Plasmid

#164497

Purpose

For bacterial expression of rat His-tagged Δ188Annexin A11

Depositor

Anni Vedeler

Article

Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

Insert

rat Δ188Annexin A11 (Anxa11 Rat)

Tags

His

Expression

Bacterial

Mutation

N-terminal truncation

Promoter

T7

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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shFGF2#1

Plasmid

#157667

Purpose

Tet inducible knockdown of FGF2

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Fibroblast growth factor 2 (FGF2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_IGLL1_C1-set

Plasmid

#109991

Purpose

Protein expression and purification of IGLL1_C1-set

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

IGLL1_C1-set (IGLL1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_RARA_Hormone-recep

Plasmid

#109836

Purpose

Protein expression and purification of RARA_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RARA_Hormone-recep (RARA Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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LIC-Z-delCry2

Plasmid

#153545

Purpose

this is the non-clustering version of LIC-Z tagged with mcherry

Depositor

Katharina Gaus

Article

Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

Insert

human TCR ζ-Chain (CD247 Human)

Tags

mCherry

Expression

Mammalian

Mutation

with Cry2 sequence deleted from LIC-Z

Promoter

CMV

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-SERCA1

Plasmid

#109552

Purpose

Multisite gateway vector for 3' tagging with sarcoplasmic/endoplasmic calcium ATPase 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

sarcoplasmic/endoplasmic calcium ATPase 1 (atp2a1 Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-MD2 (F126A)-HA

Plasmid

#133067

Purpose

Retroviral expression of mouse MD2 (F126A) tagged with HA

Depositor

Jonathan Kagan

Article

Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

Insert

MD2 (F126A) (Ly96 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

F126A

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-MD2 (C95A)-HA

Plasmid

#133066

Purpose

Retroviral expression of mouse MD2 (C95A) tagged with HA

Depositor

Jonathan Kagan

Article

Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

Insert

MD2 (C95A) (Ly96 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

C95A

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-deltaA1-R1597W-VWF

Plasmid

#124826

Purpose

Expresses multimeric human VWF protein without A1 domain and a R1597W mutation in A2 domain. This protein has C-terminal c-Myc tag.

Depositor

Sriram Neelamegham

Article

Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

Insert

VWF without A1 domain and R1597W mutation A2 domain (VWF Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-deltaA1-D1498A-VWF

Plasmid

#124825

Purpose

Expresses multimeric human VWF protein without A1 domain and a D1498A mutation in A2 domain. This protein has C-terminal c-Myc tag.

Depositor

Sriram Neelamegham

Article

Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

Insert

VWF without A1 domain and D1498A mutation A2 domain (VWF Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pMCh1809_pBS_cdc42E6-boxB

Plasmid

#118609

Purpose

cdc42E6 3'UTR-boxB tagged for T3 in vitro transcription to use in pulldown

Depositor

Marina Chekulaeva

Article

Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

Insert

cdc42E6 3'UTR (Cdc42 Mouse)

Use

In vitro transcription

Tags

boxB

Promoter

T3

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pMCh1807_pBS_cdc42E7-boxB

Plasmid

#118612

Purpose

cdc42E7 3'UTR-boxB tagged for T3 in vitro transcription to use in pulldown

Depositor

Marina Chekulaeva

Article

Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

Insert

cdc42E7 3'UTR (Cdc42 Mouse)

Use

In vitro transcription

Tags

boxB

Promoter

T3

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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