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TFORF3062

Plasmid

#144538

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF549 (ZNF549 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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YFP-cCGNL1-cCGN_D-Myc

Plasmid

#236525

Purpose

For expression of Chimera of B-region cCGN in cCGNL1 in mammalian cells

Depositor

Sandra Citi

Article

Paschoud et al J Biol Chem. 2012 Apr 13;287(16):13159-6

Insert

CGNL1-CGN (CGN Canis lupus familiaris (dog))

Tags

Myc and YFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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YFP-cCGNL1-cCGN_D-Myc

Plasmid

#236525

Purpose

For expression of Chimera of B-region cCGN in cCGNL1 in mammalian cells

Depositor

Sandra Citi

Article

Paschoud et al J Biol Chem. 2012 Apr 13;287(16):13159-6

Insert

CGNL1-CGN (CGN Canis lupus familiaris (dog))

Tags

Myc and YFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-cCGN-cCGNL1_D-Myc

Plasmid

#236523

Purpose

For expression of Chimera of D-region cCGNL1 in cCGN in mammalian cells

Depositor

Sandra Citi

Article

Paschoud et al J Biol Chem. 2012 Apr 13;287(16):13159-6

Insert

CGN-CGNL1 (CGN Canis lupus familiaris (dog))

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-cCGN-cCGNL1_D-Myc

Plasmid

#236523

Purpose

For expression of Chimera of D-region cCGNL1 in cCGN in mammalian cells

Depositor

Sandra Citi

Article

Paschoud et al J Biol Chem. 2012 Apr 13;287(16):13159-6

Insert

CGN-CGNL1 (CGN Canis lupus familiaris (dog))

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-cCGN-cCGNL1_B-Myc

Plasmid

#236522

Purpose

For expression of Chimera of B-region cCGNL1 in cCGN in mammalian cells

Depositor

Sandra Citi

Article

Paschoud et al J Biol Chem. 2012 Apr 13;287(16):13159-6

Insert

CGN-CGNL1 (CGN Canis lupus familiaris (dog))

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-cCGN-cCGNL1_B-Myc

Plasmid

#236522

Purpose

For expression of Chimera of B-region cCGNL1 in cCGN in mammalian cells

Depositor

Sandra Citi

Article

Paschoud et al J Biol Chem. 2012 Apr 13;287(16):13159-6

Insert

CGN-CGNL1 (CGN Canis lupus familiaris (dog))

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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YFP-cCGNL1-cCGN_B-Myc

Plasmid

#236524

Purpose

For expression of Chimera of D-region cCGN in cCGNL1 in mammalian cells

Depositor

Sandra Citi

Article

Paschoud et al J Biol Chem. 2012 Apr 13;287(16):13159-6

Insert

CGNL1-CGN (CGN Canis lupus familiaris (dog))

Tags

Myc and YFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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YFP-cCGNL1-cCGN_B-Myc

Plasmid

#236524

Purpose

For expression of Chimera of D-region cCGN in cCGNL1 in mammalian cells

Depositor

Sandra Citi

Article

Paschoud et al J Biol Chem. 2012 Apr 13;287(16):13159-6

Insert

CGNL1-CGN (CGN Canis lupus familiaris (dog))

Tags

Myc and YFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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hCGN-HA

Plasmid

#236508

Purpose

For expression of Cingulin (human) in mammalian cells

Depositor

Sandra Citi

Article

Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

Insert

CGN (CGN Human)

Tags

HA

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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hCGN-HA

Plasmid

#236508

Purpose

For expression of Cingulin (human) in mammalian cells

Depositor

Sandra Citi

Article

Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

Insert

CGN (CGN Human)

Tags

HA

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD D262V

Plasmid

#234607

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 D262V LCD (186-320)

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_D262V (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

C-terminal domain (186-320) bearing familial ALS …

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD D262V

Plasmid

#234607

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 D262V LCD (186-320)

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_D262V (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

C-terminal domain (186-320) bearing familial ALS …

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD D262N

Plasmid

#234608

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 D262N LCD (186-320)

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_D262N (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

C-terminal domain (186-320) bearing familial ALS …

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
A1-LCD D262N

Plasmid

#234608

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 D262N LCD (186-320)

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_D262N (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

C-terminal domain (186-320) bearing familial ALS …

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD -3G1S+4V

Plasmid

#234612

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 Gly and 1 Ser mutated to Asn

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_4GSV (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

G204V, S231V, G274V, G303V

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD -3G1S+4V

Plasmid

#234612

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 Gly and 1 Ser mutated to Asn

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_4GSV (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

G204V, S231V, G274V, G303V

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD 5W D262V

Plasmid

#234617

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262V with partial W mutant

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_5W D262V (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

F222W, F228W, Y237W, D262V, Y305W, F320W

Promoter

T7

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
A1-LCD 5W D262V

Plasmid

#234617

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262V with partial W mutant

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_5W D262V (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

F222W, F228W, Y237W, D262V, Y305W, F320W

Promoter

T7

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pADSS-EGFP

Plasmid

#228197

Purpose

expressed human ADSS with an EGFP fluorescent protein tag

Depositor

Stephen Benkovic

Article

Zhao et al J Biol Chem. 2015 Mar 13;290(11):6705-13

Insert

ADSS (ADSS1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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