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pDONR223_CGREF1_WT

Plasmid

#82895

Purpose

Gateway Donor vector containing CGREF1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

CGREF1 (CGREF1 Human)

Use

Gateway entry vector

Mutation

deletion of AA 233-249

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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R725-X20-566: His6-MBP-tev-Avi-Hs.LGALS3

Plasmid

#159700

Purpose

E. coli protein expression

Depositor

Dominic Esposito

Article

Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

Insert

His6-MBP-tev-Avi-Hs.LGALS3 (LGALS3 Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-L1CAM-COMP5AP-AviTag-9xHis

Plasmid

#157595

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

L1CAM (L1CAM Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_EIF4E_WT

Plasmid

#82112

Purpose

Gateway Donor vector containing EIF4E , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

EIF4E (EIF4E Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_DIABLO_WT

Plasmid

#82257

Purpose

Gateway Donor vector containing DIABLO , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

DIABLO (DIABLO Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-IGSF6-Fc(DAPA)-AviTag-6xHis

Plasmid

#156508

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

IGSF6 (IGSF6 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_RALA_WT_V5

Plasmid

#82988

Purpose

Gateway Donor vector containing RALA, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RALA (RALA Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_BMPR1B_WT

Plasmid

#82195

Purpose

Gateway Donor vector containing BMPR1B , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BMPR1B (BMPR1B Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KEAP1_p.G524C

Plasmid

#82779

Purpose

Gateway Donor vector containing KEAP1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KEAP1 (KEAP1 Human)

Use

Gateway entry vector

Mutation

G524C; C624S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_NFE2L2_p.G31A

Plasmid

#81524

Purpose

Gateway Donor vector containing NFE2L2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NFE2L2 (NFE2L2 Human)

Use

Gateway entry vector

Mutation

G31A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ATF2_WT

Plasmid

#82204

Purpose

Gateway Donor vector containing ATF2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ATF2 (ATF2 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_NRAS_p.Q61L

Plasmid

#81674

Purpose

Gateway Donor vector containing NRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NRAS (NRAS Human)

Use

Gateway entry vector

Mutation

Q61L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CD72-Fc(DAPA)-AviTag-6xHis

Plasmid

#156696

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CD72 (CD72 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_RB1_p.I782M

Plasmid

#81547

Purpose

Gateway Donor vector containing RB1 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RB1 (RB1 Human)

Use

Gateway entry vector

Mutation

I782M

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-DLL1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156904

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

DLL1 (DLL1 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_NFIL3_WT_V5

Plasmid

#82985

Purpose

Gateway Donor vector containing NFIL3, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

NFIL3 (NFIL3 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAT1_p.Y701F

Plasmid

#82183

Purpose

Gateway Donor vector containing STAT1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAT1 (STAT1 Human)

Use

Gateway entry vector

Mutation

Y701F

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CDC42_WT

Plasmid

#82104

Purpose

Gateway Donor vector containing CDC42 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CDC42 (CDC42 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_WIPI1_WT

Plasmid

#81894

Purpose

Gateway Donor vector containing WIPI1 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

WIPI1 (WIPI1 Human)

Use

Gateway entry vector

Mutation

T31I

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MFAP4_WT

Plasmid

#81858

Purpose

Gateway Donor vector containing MFAP4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MFAP4 (MFAP4 Human)

Use

Gateway entry vector

Mutation

A173V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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