We narrowed to 25,030 results for: Nes

1. c3GIC9n

   Plasmid

   #62192

   Purpose

   col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes

   Depositor

   Lukas Dow

   Article

   Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

   Insert

   humanized S. Pyogenes Cas9 (D10A)
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mutation

   D10A substitution (Cas9n)

   Promoter

   TRE3G

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cyan Nano-lantern/pcDNA3

   Plasmid

   #67660

   Purpose

   cyan luminescent protein for high-speed single-cell and whole body imaging

   Depositor

   Takeharu Nagai

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   Cyan Nano-lantern
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLIK 3xFLAG-TWIST2 neo

   Plasmid

   #197936

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-tagged TWIST2

   Depositor

   Kevin Janes

   Article

   Dutta et al Genome Res. 2023 Feb 21;33(3):314-31. do

   Insert

   TWIST2 (TWIST2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCerulean3-H2B-6

   Plasmid

   #55421

   Purpose

   Localization: Nucleus/Histones, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pJG85

   Plasmid

   #89281

   Purpose

   Entry vector containing the TaU6 promoter and an Esp3I Golden Gate cloning site for cloning of the sgRNA, all between attL5 and attL2 sites

   Depositor

   Daniel Voytas

   Article

   Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. TB:7xSMAD_RE:MiniP

   Plasmid

   #133879

   Purpose

   7 copies the SMAD DNA binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Insert

   7 copies the SMAD DNA binding motif
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1 shTGFBR3 puro

   Plasmid

   #58696

   Purpose

   Lentiviral shRNA vector for knockdown of human TGFBR3

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

   Insert

   shTGFBR3 (TGFBR3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

8. p5xE-Box

   Plasmid

   #118052

   Purpose

   GBPart - Operator/DNA Response element - 5 copies of the E-box motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   5 copies of the E-box motif
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKMD106h - intein-Cas9(C574)

   Plasmid

   #64191

   Purpose

   Expresses intein-Cas9(C574) in mammalian cells

   Depositor

   David Liu

   Article

   Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

   Insert

   mammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG
   

   Use

   CRISPR

   Tags

   NLS - 3x FLAG (C terminal on insert)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR221-SMAD3

    Plasmid

    #79507

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human SMAD3 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    SMAD3 (SMAD3 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    no Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant

    Plasmid

    #87350

    Purpose

    S867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET construct

    Depositor

    Jennifer Doudna

    Article

    Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

    Insert

    Cas9
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    C80S/S355C/C574S/S867C

    Promoter

    T7

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. TET-pLKO.1 PURO shSMAD4 #2

    Plasmid

    #83091

    Purpose

    Lentiviral shRNA vector for inducible knockdown of human SMAD4

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    shSMAD4 (SMAD4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

13. pSLIK_hygro_3xflag_Luciferase

    Plasmid

    #136528

    Purpose

    Lentiviral expression vector for an inducible 3xflag-tagged Luciferase

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    Luciferase
    

    Use

    Lentiviral; Destinatioin vector for gateway cloni…

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. 35S::Cel1SP-GeNL/pCAMBIA1301

    Plasmid

    #190069

    Purpose

    Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana apoplast

    Depositor

    Takeharu Nagai

    Article

    Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

    Insert

    Cel1 signal peptide-Green-enhanced Nano-lantern
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available Since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCfB3035(X-4 MarkerFree)

    Plasmid

    #73273

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-4 (Chr X: 236336..237310)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJT11

    Plasmid

    #98308

    Purpose

    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

    Insert

    TRPL3ClLIS1>TRPL41B
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Nano-lantern-plasma membrane/pcDNA3

    Plasmid

    #51975

    Purpose

    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to the plasma membrane

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Use

    Tags

    HIS and Lyn

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pP19 (GB0038)

    Plasmid

    #68195

    Purpose

    Provides the CDS of the Tomato bushy stunt virus (TBSV) silencing suppressor P19 as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    P19 coding region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCB128

    Plasmid

    #205137

    Purpose

    Expression of mCherry-2xFYVE in Dictyostelium

    Depositor

    Jason King

    Article

    Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

    Insert

    2xFYVE (from human Hrs) (HGS Synthetic)
    

    Use

    Dictyostelium expression

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Orange Nano-lantern/pRSETB

    Plasmid

    #67659

    Purpose

    orange luminescent protein for high-speed single-cell and whole body imaging

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Orange Nano-lantern
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only