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Addgene

We narrowed to 18,777 results for: IRE

Showing: 10201 - 10250 of 18777 results
  1. pGEX-2T-CBP(1-287)

    Plasmid
    #182839
    Purpose
    CBP(1-287) was inserted into pGEX-2T with NdeI and NsiI sites. pGEX-2T-CBP(1-287) was used in the GST pull-down assay described in our paper (Tie et al. 2012 MCB, Tie et al 2014 Development)
    Depositor
    Insert
    CBP1-287
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pJH3453

    Plasmid
    #179577
    Purpose
    Pglc-55B::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans AVB/others premotor IN and others expression of miniSOG-SL2 BFP
    Depositor
    Insert
    tomm20::miniSOG (tomm-20 Nematode)
    Use
    Tags
    BFP
    Expression
    Worm
    Mutation
    Promoter
    Plgc-55B
    Available since
    March 1, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
  3. pJH2890

    Plasmid
    #179561
    Purpose
    Plgc-55B::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans AVB premotor IN and other neurals expression of miniSOG-SL2 RFP
    Depositor
    Insert
    tomm20::miniSOG (tomm-20 Nematode)
    Use
    Tags
    wCherry
    Expression
    Worm
    Mutation
    Promoter
    Plgc-55B
    Available since
    March 1, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pGG422_UV5

    Plasmid
    #165607
    Purpose
    Vector for expression of the SpCas9 KG variant with sgRNA in E. coli: KG(SpCas9, D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)
    Depositor
    Insert
    lacUV5 driving Streptococcus pyogenes Cas9 KG(D1332K/R1333G) and hEGFP-sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    D1332K and R1333G mutations in SpCas9
    Promoter
    lacUV5 driving Cas9 KG and UV5 driving sgRNA
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pUE1-TSP-ORF68 K174A/R179A/K182A

    Plasmid
    #162654
    Purpose
    Expresses ORF68 K174A/R179A/K182A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site
    Depositor
    Insert
    ORF68
    Use
    Tags
    N-terminal 2xStrep-Tag II and HRV 3C site
    Expression
    Mammalian
    Mutation
    Amino acids K174, R179, and K182 mutated to alani…
    Promoter
    CMV
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pCAG-EGxxFP-CD55

    Plasmid
    #153959
    Purpose
    Used for EGxxFP assay with CD55 intron 3, exon 4, and intron 4 sequences
    Depositor
    Insert
    CD55 (a 1,016-bp fragment from intron 3, exon 4, and intron 4)
    Use
    A plasmid specific for egxxfp assay
    Tags
    N/A
    Expression
    Mammalian
    Mutation
    No mutation
    Promoter
    CAG promoter
    Available since
    Dec. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
  7. pAA2

    Plasmid
    #154162
    Purpose
    Firefly luciferase under the control of 4 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
    Depositor
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    16 wt NF-κB sites were replaced with Rel1A-specif…
    Promoter
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. pAA1

    Plasmid
    #154161
    Purpose
    Firefly luciferase under the control of 3 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
    Depositor
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    12 wt NF-κB sites were replaced with Rel1A-specif…
    Promoter
    Available since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
  9. pKM62

    Plasmid
    #154160
    Purpose
    Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel2-specific (VII) NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
    Depositor
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    4 wt NF-κB sites were replaced with Rel2-specific…
    Promoter
    Available since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
  10. pKM59

    Plasmid
    #154158
    Purpose
    Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, a TATA box and Kozak sequence of the Metchnikowin gene and additional NF-κB site.
    Depositor
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Enhancer for the Drosophila melanogaster Metchnik…
    Available since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
  11. pKM52

    Plasmid
    #154155
    Purpose
    Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel2 NF-κB site
    Depositor
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Drosophila melanogaster minimal Hsp70 promoter wi…
    Available since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
  12. pKM51

    Plasmid
    #154154
    Purpose
    Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel1A NF-κB site
    Depositor
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Drosophila melanogaster minimal Hsp70 promoter wi…
    Available since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 10201 - 10250 of 18777 results