We narrowed to 18,777 results for: IRE
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Plasmid#185245PurposeFor the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k4_1. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertPvLEA4_repeats_k4_1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_PvLEA4_repeats_k3_11 (pBS0761)
Plasmid#185243PurposeFor the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_11. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertPvLEA4_repeats_k3_11
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_DrHD_dom3_k5_0 (pBS0758)
Plasmid#185242PurposeFor the mammalian expression of the Deinococcus radiodurans protein DrHD_dom3_k5_0. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).DepositorInsertDrHD_dom3_k5_0
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_DrHD_dom3_k3_7 (pBS0755)
Plasmid#185241PurposeFor the mammalian expression of the Deinococcus radiodurans protein DrHD_dom3_k3_7. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertDrHD_dom3_k3_7
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_DrHD_dom3_k3_1 (pBS0754)
Plasmid#185240PurposeFor the mammalian expression of the Deinococcus radiodurans protein DrHD_dom3_k3_1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).DepositorInsertDrHD_dom3_k3_1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_CI016_HUMAN (pBS0747)
Plasmid#185238PurposeFor the mammalian expression of the human protein CI016_HUMAN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertCI016_HUMAN
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_AdAPOA4-44 (pBS0745)
Plasmid#185236PurposeFor the mammalian expression of the Chinese giant salamander protein AdAPOA4-44. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).DepositorInsertAdAPOA4-44
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
BPK880-pCAG-DmrC-NLS-3xFLAG-VP64
Plasmid#136912PurposeDmrC with VP64 fused to it's C-terminus; Mammalian expression vectorDepositorInsertpCAG-DmrC-NLS-3xFLAG-VP64
UseCRISPRTagsExpressionMammalianMutationPromoterChicken Beta ActinAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCaf40-A253FY208A_X
Plasmid#147973PurposeInsect Expression of DmCaf40-A253FY208ADepositorInsertDmCaf40-A253FY208A (Rcd-1 Fly)
UseTagsEGFP (A179V)ExpressionInsectMutationPromoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4E-T_1-511-LLFWKtoAAAAA-V5His6_Z
Plasmid#148160PurposeInsect Expression of Dm4E-T_1-511-LLFWKtoAAAAADepositorInsertDm4E-T_1-511-LLFWKtoAAAAA (4E-T Fly)
UseTagsExpressionInsectMutationalias: 4E-T; one non silent mutation L127S, annot…PromoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-CBP(1-287)
Plasmid#182839PurposeCBP(1-287) was inserted into pGEX-2T with NdeI and NsiI sites. pGEX-2T-CBP(1-287) was used in the GST pull-down assay described in our paper (Tie et al. 2012 MCB, Tie et al 2014 Development)DepositorInsertCBP1-287
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11-PC(75-390)-H6
Plasmid#183781PurposeExpress PC(75-390)-H6 in E. coli. PC(75-390)-H6 was used for GST-CBP pull-down assaysDepositorInsertPolycomb (w Fly)
UseTags6 His tag at the C-terminusExpressionBacterialMutation6 His tag at the C-terminusPromoterT7Available sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHelz-E958Q_AH
Plasmid#148884PurposeInsect Expression of DmHelz-E958QDepositorInsertDmHelz-E958Q (CG9425 Fly)
UseTagsExpressionInsectMutationtwo silent an two non silent G1374S, R2076G mutat…PromoterAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-83-GB1_Z
Plasmid#148180PurposeBacterial Expression of DmThor_50-83DepositorInsertDmThor_50-83 (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHelz_1213-2103_AH
Plasmid#148883PurposeInsect Expression of DmHelz_1213-2103DepositorInsertDmHelz_1213-2103 (CG9425 Fly)
UseTagsExpressionInsectMutationtwo silent an two non silent G1374S, R2076G mutat…PromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCaf40-F65AC69Y_X
Plasmid#147993PurposeInsect Expression of DmCaf40-F65AC69YDepositorInsertDmCaf40-F65AC69Y (Rcd-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCaf40_25-291_W
Plasmid#147866PurposeInsect Expression of DmCaf40_25-291DepositorInsertDmCaf40_25-291 (Rcd-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pN1-HsSmg5-G120E-del133-155-V5-SBP-MBP_V
Plasmid#147767PurposeMammalian Expression of HsSmg5-G120E-del133-155DepositorInsertHsSmg5-G120E-del133-155 (SMG5 Human)
UseTagsExpressionMammalianMutationone silent mutation C969T compared to the sequenc…PromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsC2orf68-EBMmut_K
Plasmid#146761PurposeMammalian Expression of HsC2orf68-EBMmutDepositorInsertHsC2orf68-EBMmut (C2orf68 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
cASPG1-mRFP
Plasmid#181962PurposeASPG1 protein tagged with mRFP, under control of TBA2 promoterDepositorInsertASPG1 (ASPG1 Mustard Weed)
UseTagsmRFPExpressionPlantMutationPromoterTBA2 promoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-ZNF267
Plasmid#178452Purposedoxycycline-inducible expression of Human ZNF267 in mammalian cellsDepositorInsertZNF267 (ZNF267 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-PLEK
Plasmid#178439Purposedoxycycline-inducible expression of Human PLEK in mammalian cellsDepositorInsertPLEK (PLEK Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pN1-HsSmg5-G120E-siRNAres-V5-SBP-MBP_S
Plasmid#147470PurposeMammalian Expression of HsSmg5-G120E-siRNAresDepositorInsertHsSmg5-G120E-siRNAres (SMG5 Human)
UseTagsExpressionMammalianMutationone silent mutation C969T compared to the sequenc…PromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmDCP1-Y34A-dsRNAres_P
Plasmid#147216PurposeInsect Expression of DmDCP1-Y34A-dsRNAresDepositorInsertDmDCP1-Y34A-dsRNAres (Dcp1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH3453
Plasmid#179577PurposePglc-55B::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans AVB/others premotor IN and others expression of miniSOG-SL2 BFPDepositorInserttomm20::miniSOG (tomm-20 Nematode)
UseTagsBFPExpressionWormMutationPromoterPlgc-55BAvailable sinceMarch 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJH2890
Plasmid#179561PurposePlgc-55B::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans AVB premotor IN and other neurals expression of miniSOG-SL2 RFPDepositorInserttomm20::miniSOG (tomm-20 Nematode)
UseTagswCherryExpressionWormMutationPromoterPlgc-55BAvailable sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-RB_B
Plasmid#145977PurposeInsect Expression of DmAGO1-RBDepositorInsertDmAGO1-RB (AGO1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCCR4_A
Plasmid#145894PurposeInsect Expression of DmCCR4DepositorInsertDmCCR4 (twin Fly)
UseTagsExpressionInsectMutationThe following variations comprises from our S2 ce…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NvHN-DmXRN1_1323-1355_N
Plasmid#147052PurposeBacterial Expression of DmXRN1_1323-1355DepositorInsertDmXRN1_1323-1355 (pcm Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-SPN_ARR N52R (Shank3)
Plasmid#175255PurposeSPN-ARR fragment with structure opening N52R mutation in EGFP backbone for mammalian expressionDepositorInsertSPN-ARR fragment with N52R mutation from SHANK3
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-His-TEV-OTC_d1_Ub
Plasmid#170729PurposeScaffold design for studying ubiquitin interacting proteins by cryo-EM.DepositorInsertE. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
UseTags6xHis-TEVExpressionBacterialMutationThe C-terminus of OTC was truncated by removing o…PromoterT7Available sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-3)
Plasmid#159664PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer10-UBC9-sh3
Plasmid#168987PurposeExpresses RFP cDNA and UBC9 shRNA 3 in mammalian cellsDepositorInsertUbiquitin Conjugating Enzyme 9
UseTagsExpressionMammalianMutationPromoterUbc promoterAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVMG0193_Cq-Rpl40-Cas9
Plasmid#169346PurposeExpression of Cas9 in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0193_Cq-Rpl40-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterCPIJ002413Available sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGG422_UV5
Plasmid#165607PurposeVector for expression of the SpCas9 KG variant with sgRNA in E. coli: KG(SpCas9, D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)DepositorInsertlacUV5 driving Streptococcus pyogenes Cas9 KG(D1332K/R1333G) and hEGFP-sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationD1332K and R1333G mutations in SpCas9PromoterlacUV5 driving Cas9 KG and UV5 driving sgRNAAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-2)
Plasmid#159663PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-1)
Plasmid#159662PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-1)
Plasmid#159656PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(-)-TOMM20-dTomato
Plasmid#115922Purposedonor to insert dTomato at the TOMM20 locus in human cellsDepositorInsertTOMM20-dTomato
UseDonor templateTagsdTomatoExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUE1-TSP-ORF68 K174A/R179A/K182A
Plasmid#162654PurposeExpresses ORF68 K174A/R179A/K182A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage siteDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag II and HRV 3C siteExpressionMammalianMutationAmino acids K174, R179, and K182 mutated to alani…PromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGxxFP-PIGA
Plasmid#153958PurposeUsed for EGxxFP assay with PIGA intron 5 and exon 6 sequencesDepositorInsertPIGA (a 401-bp fragment from intron 5 and exon 6)
UseA plasmid specific for egxxfp assayTagsN/AExpressionMammalianMutationNo mutationPromoterCAG promoterAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGxxFP-CD55
Plasmid#153959PurposeUsed for EGxxFP assay with CD55 intron 3, exon 4, and intron 4 sequencesDepositorInsertCD55 (a 1,016-bp fragment from intron 3, exon 4, and intron 4)
UseA plasmid specific for egxxfp assayTagsN/AExpressionMammalianMutationNo mutationPromoterCAG promoterAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAA2
Plasmid#154162PurposeFirefly luciferase under the control of 4 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutation16 wt NF-κB sites were replaced with Rel1A-specif…PromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAA1
Plasmid#154161PurposeFirefly luciferase under the control of 3 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutation12 wt NF-κB sites were replaced with Rel1A-specif…PromoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM62
Plasmid#154160PurposeFirefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel2-specific (VII) NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutation4 wt NF-κB sites were replaced with Rel2-specific…PromoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM59
Plasmid#154158PurposeFirefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, a TATA box and Kozak sequence of the Metchnikowin gene and additional NF-κB site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoterEnhancer for the Drosophila melanogaster Metchnik…Available sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM52
Plasmid#154155PurposeFirefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel2 NF-κB siteDepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoterDrosophila melanogaster minimal Hsp70 promoter wi…Available sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM51
Plasmid#154154PurposeFirefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel1A NF-κB siteDepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoterDrosophila melanogaster minimal Hsp70 promoter wi…Available sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDK616
Plasmid#134202PurposeBacterial expression of human Ska1 microtubule binding domain (MTBD) mutantDepositorInsertHis-Precission-human Ska1 MTBD (SKA1 Human)
UseTagsExpressionBacterialMutationK223A K226APromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW040
Plasmid#134127PurposeBacterial expression of human Ska1 microtubule binding domain (MTBD)DepositorInsertHis-Precission-human Ska1 MTBD (SKA1 Human)
UseTagsExpressionBacterialMutationaa 132-255 R236APromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only