We narrowed to 26,051 results for: Sis

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pDONR223-TSSK3

Plasmid

#23553

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TSSK3 (TSSK3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-NEK3

Plasmid

#23821

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK3 (NEK3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Venus

Plasmid

#105805

Purpose

Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Venus, cleavable by TEV. Useful for protein interaction analysis by FRET.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

ORF1: 3C-Cerulean; ORF2: TEV-Venus

Expression

Mammalian

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pSLPB3B-mAID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

Plasmid

#187963

Purpose

mAID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Inserts

AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
osTIR1

Use

CRISPR and Synthetic Biology

Tags

GGS linker and T2A

Expression

Mammalian

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pBABE-RPS14(WT)-Myc

Plasmid

#122235

Purpose

expresses RPS14 protein with a Myc tag in mammalian cells (puro resistance)

Depositor

Gerardo Ferbeyre

Article

Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

Insert

RPS14 (RPS14 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

vector's LTR

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pPHAGE-CR-NENF-C-TAP

Plasmid

#128511

Purpose

Lentiviral constitutive expression of CRISPR-resistant NENF with c-terminal FLAG and HA tag.

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

NENF (NENF Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Synonymous silent mutations to F81, Y82, G83 and …

Promoter

CMV

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-XRCC1-gRNA res-Neo

Plasmid

#176149

Purpose

XRCC1 with dual PAM resistance to XRCC1 gRNA1 and XRCC1 gRNA2 & a neomycin/G418 resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

X-ray repair cross complementing 1 (XRCC1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Dual PAM resistance to XRCC1 gRNA 1 and XRCC1 gRN…

Promoter

CMV

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-TSSK1

Plasmid

#23740

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TSSK1 (TSSK1B Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pEAHISMRSA0482

Plasmid

#96968

Purpose

expression of methicillin-resistant Staphylococcus aureus orf 0482

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

MRSA ORF0482

Tags

N-ter TEV protease cleavable 6His

Expression

Bacterial

Mutation

none

Promoter

T7

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-NEK4

Plasmid

#23497

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK4 (NEK4 Human)

Use

Gateway donor vector

Mutation

Short isoform, only goes to aa632

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-NEK11

Plasmid

#23816

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK11 (NEK11 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-STK17B

Plasmid

#23721

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

STK17B (STK17B Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-TSSK2

Plasmid

#23397

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TSSK2 (TSSK2 Human)

Use

Gateway donor vector

Mutation

1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-NEK10

Plasmid

#23437

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK10 (NEK10 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Amber

Plasmid

#105806

Purpose

Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Amber, cleavable by TEV. Control for protein interaction analysis by FRET.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

ORF1: 3C-Cerulean; ORF2: TEV-Amber

Expression

Mammalian

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-N1303K-ngRNA+14_EF1a-puroR

Plasmid

#207359

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of N1303K-CFTR via prime editing and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

N1303K-CFTR +14 ngRNA

Use

Lentiviral

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-L227R-ngRNA+15_EF1a-puroR

Plasmid

#207360

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of L227R-CFTR via prime editing and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

L227R-CFTR +15 ngRNA

Use

Lentiviral

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-V1-rCD20-BSD

Plasmid

#209752

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 1) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-V2-rCD20-BSD

Plasmid

#209753

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 2) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-V3-rCD20-BSD

Plasmid

#209754

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 3) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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