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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,956 results for: codon optimized

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Showing: 4081 - 4100 of 4956 results

  1. SpCas9 iFE

    Plasmid
    #190138
    Purpose
    Expresses SpCas9-POLB (iFE)
    Depositor
    Timothy Lu
    Publication
    Nakade et al bioRxiv 2022.12.05.518807
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-POLB (HiFi iFE)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Codon Optimized for mammalian cells
    Promoter
    CBh
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    190138-plasmid-map-sequence-id-374863

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  2. pSCL.139

    Plasmid
    #184994
    Purpose
    Express Cas9-P2A-Eco1RT in human cells
    Depositor
    Seth Shipman
    Publication
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-P2A-Eco1RT
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    Human codon optimized RT
    Promoter
    TetOn-3g
    Available since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184994-plasmid-map-sequence-id-376613

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  4. pKG113

    Plasmid
    #63073
    Purpose
    Retroviral expression of EGFP-TEV-S-tag CENP-T for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    CENP-T (CENPT Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    E. coli codon optimized. G217R mutation compared …
    Available since
    Jan. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63073-plasmid-map-sequence-id-134725

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  7. pCEP4-BaLgp160-754*

    Plasmid
    #123212
    Purpose
    Mammalian expression plasmid for Env from the BaL HIV-1 isolate; C-terminal truncation
    Depositor
    Erik Procko
    Publication
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIV-1 (BaL) Env
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Premature stop co…
    Promoter
    CMV
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    123212-plasmid-map-sequence-id-235146

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  8. pCas3-APOBEC1

    Plasmid
    #229536
    Purpose
    pMV_hyg encoding Cas3(wt)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Publication
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    rAPOBEC1
    Cas3
    Uracil glycosylase inhibitor
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    229536-plasmid-map-sequence-id-458911

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  9. pDIV303

    Plasmid
    #204978
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7 and amdS selection marker
    Depositor
    Uffe Mortensen
    Publication
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    amdS
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    two silent mutations (nt177T>C, nt879C>T)
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    204978-plasmid-map-sequence-id-415579

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  10. pNMSB84

    Plasmid
    #199309
    Purpose
    MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605
    Depositor
    Michael Krieg
    Publication
    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4
    Insert
    flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
    Expression
    Worm
    Mutation
    mTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…
    Promoter
    flp-18
    Available since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    199309-plasmid-map-sequence-id-404774

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  13. p28-2iDP-puro

    Plasmid
    #88893
    Purpose
    Dual-intron DMD platform plasmid. Co-expresses DMD platform segment, firefly luciferase, puroR and mCherry. Plasmid carries phiC31 and Bxb1 attB sites.
    Depositor
    Michele Calos
    Publication
    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Dual-intron DMD platform
    luciferase
    puromycin resistance enzyme
    mCherry
    Expression
    Mammalian
    Mutation
    Truncated version of the dystrophin protein (tran…
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    88893-plasmid-map-sequence-id-201484

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  14. pBJG1 T7-8xHis-OGT D554N

    Plasmid
    #154279
    Purpose
    Bacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Publication
    Janetzko et al Nat Chem Biol. 2016 Nov;12(11):899-901.
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    Aspartate 554 changed to Asparagine- bacterial co…
    Available since
    Sept. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    154279-plasmid-map-sequence-id-304834

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  15. pcDNA5-FRT-TO-FH-Nsp10

    Plasmid
    #157707
    Purpose
    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Publication
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FH-Nsp10 (ORF1ab SARS-CoV-2)
    Tags
    Flag-His8
    Expression
    Mammalian
    Mutation
    codon optimized
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157707-plasmid-map-sequence-id-307872

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  16. pcDNA5-FRT-TO-FH-Nsp9

    Plasmid
    #157704
    Purpose
    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Publication
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FH-Nsp9 (ORF1ab SARS-CoV-2)
    Tags
    Flag-His8
    Expression
    Mammalian
    Mutation
    codon optimized
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157704-plasmid-map-sequence-id-307868

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  17. pJW1592

    Plasmid
    #121059
    Purpose
    GFP^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Publication
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    GFP^SEC^BioTag::AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…
    Expression
    Worm
    Available since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    121059-plasmid-map-sequence-id-235964

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  19. pdnCas3-APOBEC1

    Plasmid
    #229537
    Purpose
    pMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Publication
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    rAPOBEC1
    dnCas3
    Uracil glycosylase inhibitor
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Available since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    229537-plasmid-map-sequence-id-459005

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  20. pCEP4-BG505.SOSIP-gp160-754*

    Plasmid
    #123274
    Purpose
    Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations); C-terminal truncation
    Depositor
    Erik Procko
    Publication
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIV-1 (BG505) Env
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; SOSIP mutations; …
    Promoter
    CMV
    Available since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    123274-plasmid-map-sequence-id-235237

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Showing: 4081 - 4100 of 4956 results