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pMOS013E: Entry vector for: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

Plasmid

#163073

Purpose

Entry vector for: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

mito-roGFP2-Grx1

Use

Gateway Cloning

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS015E: Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

Plasmid

#163076

Purpose

Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

mito-roGFP2-Orp1

Use

Gateway Cloning

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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LZF1604V2: CAG-spine-jRGECO1a-P2A-QuasAr3-dark Citrine

Plasmid

#104122

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

spine-jRGECO1a-P2A-QuasAr-dark Citrine

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-Kaede (Kaede-A69T)

Plasmid

#99228

Purpose

Bacterial expression of the primed conversion capable protein pr-Kaede (Kaede-A69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-Kaede

Expression

Bacterial

Mutation

A69T for primed conversion applications

Promoter

T7

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-DIO-erHRP(N175S mutant)

Plasmid

#79906

Purpose

Adeno-associated virus plasmid, Cre-dependent FLEX switch, erHRP(N175S mutant)

Depositor

Joshua Sanes

Article

Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

Insert

erHRP(N175S mutant)

Use

AAV and Cre/Lox

Tags

HA tag

Expression

Mammalian

Mutation

N175S mutation

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCIG AKT3 E17K

Plasmid

#73050

Purpose

Human AKT3 E17K

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

AKT3 E17K (AKT3 Human)

Expression

Mammalian

Mutation

E17K

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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ORAI1-hBACCS2

Plasmid

#67626

Purpose

Mammalian expression of ORAI1-hBACCS2, a fusion protein of ORAI1 and human blue light-activated Ca2+ channel switch

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

ORAI1-hBACCS2

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FLEX-rc[Chronos-GFP]

Plasmid

#62725

Purpose

AAV-mediated expression of Chronos-GFP under the EF1α promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

EF1α

Available Since

April 30, 2015

Availability

Academic Institutions and Nonprofits only

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pT7-Venus-Emi1 (914)

Plasmid

#39854

Depositor

Jonathon Pines

Article

Di Fiore et al J Cell Biol. 2007 May 7;177(3):425-37.

Insert

Emi1 (FBXO5 Human)

Use

In vitro transcription

Tags

Venus

Promoter

T7

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only

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p6903 PHAGE-B CMV-N-EGFP Myc

Plasmid

#37608

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

Myc (MYC Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only
OPN(-213)-luc

Plasmid

#31107

Depositor

Gerhart Ryffel

Article

Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

Insert

osteopontin promoter (SPP1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

promoter region -213 to +87

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV4-Tax(M20)

Plasmid

#23285

Depositor

Warner Greene

Article

Smith et al Genes Dev. 1990 Nov . 4(11):1875-85.

Insert

Tax(M20)

Expression

Mammalian

Mutation

T130A L131S

Available Since

May 3, 2010

Availability

Academic Institutions and Nonprofits only
pRSET FLIPglu-2uDelta13

Plasmid

#13561

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16F D152A Delta13

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

F16A, D152A in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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ACER

Pooled Library

#226117

Purpose

Contains 748 traditional and newly predicted epigenetic regulators using epigenetic network analyses that integrated gene codependency (DepMap) and protein-protein interaction data (BioPlex).

Depositor

Rui Lu

Article

Zhou et al Blood. 2024 Aug 16:blood.2023023644. doi

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only
"AND-gate" receiver plasmid

Plasmid

#127676

Purpose

Encodes the transcriptional activator LuxR and GFP. LuxR has an pLlacO-1 promoter and the GFP has a pLux promoter.

Depositor

Friedrich Simmel

Article

Schwarz-Schilling et al Integr Biol (Camb). 2016 Apr 18;8(4):564

Insert

LuxR, GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1 for LuxR, pLux for GFP

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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mTFP1-Clathrin-15

Plasmid

#55471

Purpose

Localization: Clathrin Vesicles, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Clathrin (CLTB Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
Cry2(1-531)-mCh-BAXS184E

Plasmid

#117238

Purpose

Light activated recruitment of BAX in mammalian cells with Cry2/CIB optogenetic switch

Depositor

Robert Hughes

Article

Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

Insert

Cry2531-mCh-BAXS184E

Expression

Mammalian

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-Cx35-Halo-C-end

Plasmid

#71512

Purpose

Mammalian expression plasmid encoding perch Cx35 with a C-terminal HaloTag.

Depositor

John O'Brien

Article

Wang et al J Cell Sci. 2015 Nov 1;128(21):3888-97.

Insert

Connexin 35

Tags

HaloTag

Expression

Mammalian

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pTetR-LasR-pLuxR-GFP

Plasmid

#171159

Purpose

Sensing Device with Reporter protein

Depositor

Matthew Chang

Article

Saeidi et al Mol Syst Biol. 2011 Aug 16;7:521. doi: 1

Inserts

lasR
gfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLux and pTet

Available Since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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modified pcAT7-Glo1

Plasmid

#160996

Purpose

Splicing reporter backbone

Depositor

Brenton Graveley

Article

Adamson et al Genome Biol. 2018 Jun 1;19(1):71. doi: 1

Insert

hemoglobin subunit beta (HBB Human)

Expression

Mammalian

Mutation

Repeated intron 1 with 40 nucelotide deletion

Promoter

CAG

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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