We narrowed to 6,370 results for: wus;

1. pET-A3A(N57Q)-BE3

   Plasmid

   #158157

   Purpose

   T7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLS

   Depositor

   Keith Joung

   Article

   Zeng et al Nat Med. 2020 Apr;26(4):535-541. doi: 10

   Insert

   6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
   

   Use

   Tags

   6xHis tag on N-terminus

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiCRISPR v2-sgACSL4

   Plasmid

   #186024

   Purpose

   knock out ACSL4 in mammalian cells

   Depositor

   Boyi Gan

   Article

   Mao et al Nature. 2021 May;593(7860):586-590. doi:

   Insert

   Acyl-CoA Synthetase Long Chain Family Member 4 (ACSL4 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV.CBA.YFP.miR-E_shControl

   Plasmid

   #180386

   Purpose

   Producing AAV that encodes negative control shRNA with miR-E backbone

   Depositor

   Huda Zoghbi

   Article

   Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

   Insert

   shControl
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mutation

   Promoter

   CBA

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-Puro-mGSDME F2A

   Plasmid

   #154878

   Purpose

   Expresses mGSDME F2A in mammalian cells

   Depositor

   Judy Lieberman

   Article

   Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

   Insert

   GSDME (Gsdme Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   changes Phenylalanine 2 to Alanine

   Promoter

   CMV

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiCRISPR v2 RB1 sgRNA #2

   Plasmid

   #202519

   Purpose

   Expressing Cas9 and sgRNA targeting human RB1 gene

   Depositor

   Ming Chen

   Article

   Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

   Insert

   RB1 sgRNA (RB1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. p23-NES-AdmCas13d-msfGFP-NES-Flag

   Plasmid

   #165075

   Purpose

   overexpression of AdmCas13d in human cells

   Depositor

   Ling-Ling Chen

   Article

   Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

   Insert

   AdmCas13d
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUW-TetO-Tead4

   Plasmid

   #128832

   Purpose

   dox-dependent expression of mouse Tead4

   Depositor

   Yossi Buganim

   Article

   Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

   Insert

   Tead4 (Tead4 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-GAL4-IL35-IRES-mCherry-PGK-BFP

   Plasmid

   #223210

   Purpose

   Lentiviral vector - mCherry reporter and IL35 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

   Depositor

   Wendell Lim

   Article

   Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

   Insert

   IL35 (IL12A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL4.15_pSOX17

   Plasmid

   #195496

   Purpose

   Firefly luciferase promoter expression vector driven only by the promoter element of human SOX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   Promoter region of SOX17 (SOX17 Human)
   

   Use

   Tags

   luc2-hPEST

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-GAL4-PDL1-IRES-mCherry-PGK-BFP

    Plasmid

    #223208

    Purpose

    Lentiviral vector - mCherry reporter and PDL1 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

    Depositor

    Wendell Lim

    Article

    Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

    Insert

    PDL1 (CD274 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-haA3A-CBE-VA

    Plasmid

    #220893

    Purpose

    Express haA3A-CBE-VA (with SpCas9) in mammalian cells

    Depositor

    Dali Li

    Article

    Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

    Insert

    haA3A-CBE-VA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-haA3A-CBE-VA-NG

    Plasmid

    #220896

    Purpose

    Express haA3A-CBE-VA-NG (with SpCas9-NG variant) in mammalian cells

    Depositor

    Dali Li

    Article

    Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

    Insert

    haA3A-CBE-VA-NG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV_Actb HMEJ donor

    Plasmid

    #97318

    Purpose

    HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb HMEJ donor
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX6P2-MDMX (23-111)

    Plasmid

    #62064

    Purpose

    Expression of human MDM4 (residues 23-111) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    MDM4 (MDM4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains residues 23-111

    Promoter

    tac

    Available Since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

15. CALID

    Plasmid

    #169708

    Purpose

    Cre-activated light-inducible Dre recombinase

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    a double-floxed inverted Dre246-nMag-T2A-pMag-Dre247
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-BTLA (Y226F, Y243F, Y257F, Y282F)-mGFP

    Plasmid

    #180792

    Purpose

    Expression of human BTLA (Y226F, Y243F, Y257F, Y282F) fused to mEGFP

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    BTLA (BTLA Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    Y226F, Y243F, Y257F, Y282F

    Promoter

    SFFV

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV_Actb NHEJ donor

    Plasmid

    #97316

    Purpose

    NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb NHEJ donor
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. APEX2-6xHp

    Plasmid

    #214411

    Purpose

    Lipid droplet localization of APEX2-6xHp for electron microscopy

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    6x Spastin aa 43-92 (APEX2 Human)
    

    Use

    Tags

    APEX2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-SV40-Galpha-s ONE-GO

    Plasmid

    #204327

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    SV40 Galpha-s ONE-GO (GNAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pYPQ265F2

    Plasmid

    #164722

    Purpose

    Gateway entry clone (attL1 & attR5) A3A/Y130F-nCas9-NG-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    A3A(Y130F)-zCas9(D10A)NG-UGI
    

    Use

    CRISPR; Gateway compatible a3a/y130f-ncas9-ng-cbe…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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