We narrowed to 7,284 results for: Ank

1. INCTbiosyn-p35S-GFP(rc)2

   Plasmid

   #127521

   Purpose

   Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 2 attachment sites.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   EGFP reverse complement coding sequence flanked by attB/attP Integrase 2 attachment sites.
   

   Expression

   Plant

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. INCTbiosyn-pEF-GFP(rc)4

   Plasmid

   #127505

   Purpose

   Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 4 attachment sites.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   EGFP reverse complement coding sequence flanked by attB/attP Integrase 4 attachment sites.
   

   Expression

   Mammalian

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAD100_hIRG1_4-461_pvp008

   Plasmid

   #124875

   Purpose

   Expresses hCAD Arg273His mutant in E.coli

   Depositor

   Konrad Buessow

   Article

   Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

   Insert

   cis-aconitate decarboxylase (ACOD1 Human)
   

   Tags

   StrepTag and TEV site

   Expression

   Bacterial

   Mutation

   mutated Arg273 to His, deleted amino acids 1-3 an…

   Promoter

   T7

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEMS1710

   Plasmid

   #29296

   Purpose

   Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

   Depositor

   Elizabeth Simpson

   Article

   de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

   Insert

   Ple238 (TRH Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   Intron-LacZ-NLS

   Available Since

   June 10, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pCMV-PEmax-P2A-GFP

   Plasmid

   #180020

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PEmax-P2A-EGFP
   

   Tags

   P2A-EGFP, SV40 bpNLS, and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript

   Promoter

   CMV

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. HSPB1

   Plasmid

   #155670

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   HSPB1 (HSPB1 Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-PE7-P2A-GFP

   Plasmid

   #222996

   Purpose

   Mammalian expression of SpCas9 PE7 prime editor with P2A-EGFP marker

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7-P2A-EGFP
   

   Tags

   P2A-EGFP, SV40 bpNLS, and c-Myc NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTet-O-Ngn2-puro

   Plasmid

   #52047

   Purpose

   3rd generation lentiviral vector, expresses Ngn2 and puromycin resistent gene under control of TetON promoter

   Depositor

   Marius Wernig

   Article

   Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10

   Insert

   Ngn2 (Neurog2 Mouse)
   

   Use

   Lentiviral

   Tags

   T2A-and puromycin resistant cassette

   Expression

   Mammalian

   Promoter

   tet-on

   Available Since

   April 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-PE7-P2A-BSD

   Plasmid

   #224122

   Purpose

   Mammalian expression of SpCas9 PE7 prime editor with P2A-BSD marker

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7-P2A-BSD
   

   Tags

   P2A-BSD, SV40 bpNLS, and c-Myc NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEYFP-N3-EPAC1

    Plasmid

    #113110

    Purpose

    Human EPAC1 gene was fused in-frame and upstream from the enhanced YFP gene in pEYFP-N3 vector (Clonetech).

    Depositor

    Xiaodong Cheng

    Article

    Mei et al J Biol Chem. 2002 Mar 29;277(13):11497-5

    Insert

    RapGEF3 (RAPGEF3 Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    PCMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5-FRT/TO-eGFP-msMMS22L

    Plasmid

    #221014

    Purpose

    mammalian expression vector of eGFP tagged mouse MMS22L

    Depositor

    Daniel Durocher

    Article

    O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

    Insert

    MMS22L (Mms22l Mouse)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    naturally siResistant to siMMS22L (human) aagacuu…

    Promoter

    CMV

    Available Since

    Sept. 5, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pEVOL-pAzF[TAG]

    Plasmid

    #164579

    Purpose

    Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons

    Depositor

    Ryan Mehl

    Article

    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

    Inserts

    Mj pCNFRS[TAG] (aaRS1)
    Mj pCNFRS[TAG] (aaRS2)
    Mj tRNA[TAG]
    

    Expression

    Bacterial

    Mutation

    Y32L L65V F108W Q109M D158G I159P

    Promoter

    araBAD, glnS, and proK

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5-F-Med26-NTD R61A K62A

    Plasmid

    #49256

    Purpose

    Mammalian expression of flag-tagged human Med26-NTD R61A K62A

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

    Insert

    Med 26 (MED26 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    NTD R61A K62A

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

14. Str-KDEL_CD9-SBP-mCherry

    Plasmid

    #222329

    Purpose

    Synchronize the trafficking of CD9 from the ER.

    Depositor

    Franck Perez

    Article

    Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

    Insert

    Streptavidin-KDEL and CD9 fused to SBP-mCherry (CD9 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. Str-KDEL_CD9-SBP-EGFP

    Plasmid

    #222328

    Purpose

    Synchronize the trafficking of CD9 from the ER.

    Depositor

    Franck Perez

    Article

    Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

    Insert

    Streptavidin-KDEL and CD9 fused to SBP-EGFP (CD9 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1/Puro-CAG-JEDI-2P

    Plasmid

    #179458

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)

    Depositor

    Francois St-Pierre

    Article

    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

    Insert

    JEDI-2P
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-kz-CD20-Puro

    Plasmid

    #209759

    Purpose

    Lentiviral transfer plasmid to express the CDS of the human CD20 gene, MS4A1.

    Depositor

    Andrei Thomas-Tikhonenko

    Article

    Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

    Insert

    MS4A1 (MS4A1 Human)
    

    Use

    Lentiviral

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. GAPDH

    Plasmid

    #155622

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    GAPDH (GAPDH Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pINTO-N3::hTET3

    Plasmid

    #136364

    Purpose

    Expresses epitope-tagged human TET3 WT in mammalian cells

    Depositor

    Roberto Bonasio

    Article

    Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

    Insert

    TET3 (TET3 Human)
    

    Tags

    2xStrep-tagII, FLAG, and HA

    Expression

    Mammalian

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. PE7_AAVS1_knockin_HDR_donor

    Plasmid

    #223001

    Purpose

    HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Hdr donor

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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