We narrowed to 4,454 results for: TIL

1. Reb_82 aka PAS623

   Plasmid

   #71228

   Purpose

   Reb_1 with RebB(S82P, D88G)

   Depositor

   Pamela Silver

   Article

   Polka et al ACS Synth Biol. 2016 Feb 3.

   Insert

   Reb locus
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   RebB(S82P, D88G), RebA (G53A)

   Promoter

   Available since

   April 7, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pLG231

   Plasmid

   #124369

   Purpose

   IPTG inducible system (gfpmut2 with Ppsank and riboJ) for expression in B. subtilis

   Depositor

   Christopher Voigt

   Article

   Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

   Insert

   gfpmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pspank

   Available since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNGFP-EU

   Plasmid

   #161967

   Purpose

   Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).

   Depositor

   Eric Gouaux

   Article

   Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1

   Type

   Empty backbone

   Use

   Tags

   EGFP His8

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCGFP-EU

   Plasmid

   #161968

   Purpose

   Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).

   Depositor

   Eric Gouaux

   Article

   Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1

   Type

   Empty backbone

   Use

   Tags

   EGFP His8

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLG320

   Plasmid

   #124370

   Purpose

   Vanillic acid inducible system for expression in B. subtilis

   Depositor

   Christopher Voigt

   Article

   Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

   Insert

   gfpmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pspank(V)

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHoss1

   Plasmid

   #63158

   Purpose

   Suicide plasmid for efficient gene mutation in Gram-positive bacteria

   Depositor

   Attila Karsi

   Article

   Abdelhamed et al Plasmid. 2015 May 31;81:1-8. doi: 10.101

   Insert

   anti-secY-Pxyl/tetO-tetR
   

   Use

   Suicide plasmid for gram-positive bacteria

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLG166

   Plasmid

   #124366

   Purpose

   Gfpmut2 constitutively expressed using the Ppen promoter for expression in B. subtilis

   Depositor

   Christopher Voigt

   Article

   Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

   Insert

   gfpmut2 (NEWENTRY Synthetic)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ppen

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLG169

   Plasmid

   #124367

   Purpose

   Xylose inducible system with the CRE site for expression in B. subtilis

   Depositor

   Christopher Voigt

   Article

   Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

   Insert

   gfpmut2 (NEWENTRY Synthetic)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pxyl

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHH04_32_sRNA-A_Sp_coD

   Plasmid

   #120900

   Purpose

   the component plasmid of a regulated device

   Depositor

   Domitilla Del Vecchio

   Article

   Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi:

   Inserts

   pECF32 promoter [i.e., Pecf32_1122 (-60 to -1)]
   sRNA-A
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. raft-APEX2

    Plasmid

    #205089

    Purpose

    Expresses lipid-raft targeted APEX2 for proximity proteomics. Vector: pcDNA™4/TO. Insert: lipid raft target sequence (MGCVCSS) + mCherry +V5 (GKPIPNPLLGLDST) +APEX2

    Depositor

    Pieta Mattila

    Insert

    APEX2 (raft-mCherry-V5 tagged)
    

    Use

    Tags

    lipid raft targeting sequence (MGCVCSS), mCherry,…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. CD4d3+4-blac

    Plasmid

    #32403

    Depositor

    Gavin Wright

    Article

    Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

    Type

    Empty backbone

    Use

    Tags

    beta-lactamase, pentamerization domain of rat car…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. Yeast Secrete and Detect

    Plasmid Kit

    #1000000166

    Purpose

    Yeast Golden Gate cloning for protein secretion in Saccharomyces cerevisiae and Pichia pastoris. Methanol utilization (MUT) promoters, signal peptides, C-terminal tags, episomal and integrative.

    Depositor

    Sylvestre Marillonnet

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

13. pCRISPR-Cas9-ScaligD

    Plasmid

    #125688

    Purpose

    Gene knockout/in for actinomycetes

    Depositor

    Tilmann Weber

    Article

    Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

    Insert

    streptomyces codon optimized spCas9, sgRNA casette, and ScaligD
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    ermE*/tipA

    Available since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSEVA3b61

    Plasmid

    #58310

    Purpose

    Shuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner.

    Depositor

    Tom Ellis

    Article

    Wright et al ACS Synth Biol. 2014 May 13.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. K560GFP_17NL

    Plasmid

    #129767

    Purpose

    Expresses Drosophila Kinesin-1 with extended neck linker (DAL insert) truncated at 560 and GFP tagged for motility assays

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Use

    Tags

    GFP and His6

    Expression

    Bacterial

    Mutation

    DAL insert before A345

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMG13

    Plasmid

    #122932

    Purpose

    Expression of the regulator tTA.

    Depositor

    Attila Becskei

    Article

    Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

    Insert

    tTA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Pcln3

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. TU6m-gRNAscaffHygroR

    Plasmid

    #165485

    Purpose

    S. pyogenes Cas9 guide RNA expression in tilapia cells

    Depositor

    Dietmar Kueltz

    Article

    Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Oreochromis mossambicus U6

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSV_238

    Plasmid

    #122941

    Purpose

    Expression of the repressor to control the insertional promoter.

    Depositor

    Attila Becskei

    Article

    Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

    Insert

    rTetR.SUM1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Pret2

    Available since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRISPR-dCas9

    Plasmid

    #125687

    Purpose

    Gene knockdown for actinomycetes

    Depositor

    Tilmann Weber

    Article

    Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

    Insert

    streptomyces codon optimized spdCas9, sgRNA casette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    ermE*/tipA

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJB06

    Plasmid

    #190480

    Purpose

    Base Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis system

    Depositor

    Joseph Sorg

    Article

    Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

    Inserts

    Cas9
    XylR
    

    Use

    E. coli / b. subtilis - c. difficile shuttle vect…

    Tags

    Expression

    Mutation

    Promoter

    xylR-driven promoter

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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