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Flag TSC2 2A(S939A + S981A)

Plasmid

#12131

Depositor

Cheryl Walker

Article

Cai et al J Cell Biol. 2006 Apr 24. 173(2):279-89.

Insert

TSC2 S939A S981A (TSC2 Human)

Tags

Flag

Expression

Mammalian

Mutation

S939A. S981A.

Available Since

July 6, 2006

Availability

Academic Institutions and Nonprofits only
pIRES-hrGFP II-TET1

Plasmid

#83568

Purpose

Expresses TET1 in mammalian cells

Depositor

Jean-Pierre Issa

Article

Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

Insert

Tet Methylcytosine Dioxygenase 1 (TET1 Human)

Tags

3X FLAG tag and hr GFP II

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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FFA2-Tango

Plasmid

#66281

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

FFA2 (FFAR2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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DRD5-Tango

Plasmid

#66272

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

DRD5 (DRD5 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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CHRM2-Tango

Plasmid

#66249

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

CHRM2 (CHRM2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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CHRM3-Tango

Plasmid

#66250

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

CHRM3 (CHRM3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pSG5 EDD

Plasmid

#37191

Depositor

Darren Saunders, Charles Watts

Article

Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

Insert

EDD (UBR5 Human)

Tags

6x His Tag and FLAG

Expression

Mammalian

Mutation

K503R (please see depositor comments below)

Promoter

SV40

Available Since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only

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MSCV-N 16E5

Plasmid

#37874

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

HPV 16 E5 (E5 )

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

July 25, 2012

Availability

Academic Institutions and Nonprofits only
pGEX 4T1 hBad

Plasmid

#35567

Depositor

Harish Srinivas

Article

Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

Insert

bad (BAD Human)

Tags

Flag

Expression

Bacterial

Available Since

March 26, 2012

Availability

Academic Institutions and Nonprofits only
pDEST12.2 DYRK2 WT

Plasmid

#20005

Depositor

Anjana Rao

Article

Gwack et al Nature. 2006 Jun 1. 441(7093):646-50.

Insert

DYRK2 (DYRK2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pDest490-OPN-a

Plasmid

#17590

Depositor

Xin Wang

Article

Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

Insert

OPN-a (SPP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Base pairs 49–942

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
HSD11B1-FLAG

Plasmid

#24096

Depositor

Moses Chao

Article

Jeanneteau et al Proc Natl Acad Sci U S A. 2008 Mar 25. 1

Insert

HSD11beta1 (HSD11B1 Human)

Use

Lentiviral

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
FLAG-MED6

Plasmid

#15411

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

Insert

MED6 (Med6 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-MED20

Plasmid

#15421

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED20 (Med20 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-MED30

Plasmid

#15426

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED30 (Med30 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-Nore1

Plasmid

#1975

Depositor

Joseph Avruch

Article

Ortiz-Vega et al Oncogene 2002 Feb 21;21(9):1381-90.

Insert

Nore1 (Rassf5 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pET29a-DDX53

Plasmid

#222205

Purpose

Express DDX53 protein in bacteria

Depositor

Yuliang Wu

Insert

DDX53 (DDX53 Human)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-DDX53

Plasmid

#222240

Purpose

Express DDX53 protein in bacteria

Depositor

Yuliang Wu

Insert

DDX53 (DDX53 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAS_4xMx PylT_FLAG-Mx PylRS

Plasmid

#140011

Purpose

Plasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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p-EGFP-C1-Flag-mFmr1(D)

Plasmid

#87914

Purpose

Expresses mouse FMRP-EGFP fusion with phosphorylation mimic S499D.

Depositor

Stephanie Ceman

Article

Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

Insert

Fmr1 (Fmr1 Mouse)

Tags

Flag and eGFP

Expression

Mammalian

Mutation

S499D

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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