We narrowed to 18,769 results for: puro

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pRCA594 - pBA904 Puro-T2A-BFP

Plasmid

#238170

Purpose

Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Publication

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

Non-targeting sgRNA

Use

CRISPR and Lentiviral

Tags

mTagBFP2

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-2xFLAG-2xSTREP-E2F1

Plasmid

#236436

Purpose

stable expression of E2F1 using virus infection

Depositor

Michele Pagano

Publication

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F1 (E2F1 Human)

Tags

FLAG-FLAG-STREP-STREP

Expression

Mammalian

Available since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-SARS-CoV-2_Spike_WT-VA

Plasmid

#191216

Purpose

Lentiviral expression of SARS-CoV-2 Spike_WT in mammalian cells

Depositor

Mohan Babu

Publication

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

SPIKE_SARS2_WT (S SARS CoV-2)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Promoter

CMV

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-SARS-CoV-2_Spike_Beta-VA

Plasmid

#191218

Purpose

Lentiviral expression of SARS-CoV-2 Spike_Beta in mammalian cells

Depositor

Mohan Babu

Publication

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

SPIKE_SARS2_Beta (S SARS CoV-2)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

D80A D215G (Del 241-243) K417N E484K N501Y D614G …

Promoter

CMV

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-SARS-CoV-2_NSP3-VA

Plasmid

#191214

Purpose

Lentiviral expression of SARS-CoV-2 NSP3 in mammalian cells

Depositor

Mohan Babu

Publication

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

Non structural protein 3 (ORF1ab SARS CoV-2)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Promoter

CMV

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-SARS-CoV-2_NSP16-VA

Plasmid

#191215

Purpose

Lentiviral expression of SARS-CoV-2 NSP16 in mammalian cells

Depositor

Mohan Babu

Publication

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

Non structural protein 16 (ORF1ab SARS CoV-2)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Promoter

CMV

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX MLKL(1-180)-2A-mCherry-puro

Plasmid

#231976

Purpose

Tet inducible expression of DmrB-Mlkl with mCherry to induce MLKL 1-180 necroptosis

Depositor

Brian Ruffell

Publication

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

MLKL 1-180 construct with bi-cistronic mCherry (Mlkl Mouse)

Use

Lentiviral

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pScalps-Puro-FLAG_HA-Regnase-1 D141N

Plasmid

#222663

Purpose

Lentiviral vector to express mouse Regnase-1 D141N mutant tagged with FLAG-HA at N-term

Depositor

Silvia Monticelli

Publication

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12a (Zc3h12a Mouse)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

mutated aspartic acid 141 to asparagine to abroga…

Promoter

SFFV

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-GFP-Puro_TAL1-long

Plasmid

#210642

Purpose

Overexpression of TAL1-long

Depositor

Maayan Salton

Publication

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1-long (TAL1 Human)

Use

Lentiviral

Tags

GFP

Available since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 PPP2CA g1

Plasmid

#170822

Purpose

PiggyBac Cas13d sgRNA plasmid for PPP2CA knockdown

Depositor

Xiaoping Bao

Publication

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d PPP2CA gRNA1

Use

Piggybac transposon

Expression

Mammalian

Promoter

U6

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)

Plasmid

#221552

Purpose

Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

Depositor

Shawn Ferguson

Publication

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

gRNA targeting human Rab7A (RAB7A Human)

Expression

Mammalian

Promoter

U6

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro 2xStrep-HPC4-Gb5

Plasmid

#218177

Purpose

Used to make a lenti virus for stable expression of the G protein beta 5 subunit in mammalian cells

Depositor

Barry Willardson

Publication

Wang et al Mol Cell. 2023 Nov 2;83(21):3852-3868.e6

Insert

G beta subunit 5 (GNB5 Human)

Use

Lentiviral

Tags

2x Strep tag and HPC4

Expression

Mammalian

Available since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-Halo-hMRE11(H129N)

Plasmid

#208394

Purpose

To generate stable cell line expressing Halo-human MRE11(H129N) by Retroviral infection.

Depositor

Gaorav Gupta

Publication

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

Human MRE11 (MRE11 Human)

Use

Luciferase and Retroviral

Expression

Mammalian

Mutation

H129N

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-GFP-Puro_TAL1-short

Plasmid

#210641

Purpose

Overexpression of TAL1-short

Depositor

Maayan Salton

Publication

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1-short (TAL1 Human)

Use

Lentiviral

Tags

GFP

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TetOne-Puro-p62 deltaUBA

Plasmid

#204550

Purpose

Expresses p62 deltaUBA in a doxycycline-dependent manner in mammalian cells

Depositor

Masaaki Komatsu

Publication

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

p62 (SQSTM1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

deltaUBA

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pU6-sgCtrl_EF1a-Puro-T2A-BFP

Plasmid

#195500

Purpose

Lentiviral BFP expression vector bearing a sgRNA targeting a randomized human TSS sequence

Depositor

Alexander Meissner

Publication

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

sgRNA

Use

CRISPR and Lentiviral

Tags

BFP, Puro, and T2A

Expression

Mammalian

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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LentiU6-hMYOD1 gRNA_multi1-3-MS2-Puro

Plasmid

#192683

Purpose

Lentiviral expression of multi gNAs targeting hMYOD1 promoter to activate human MYOD1 transcription

Depositor

Jeffrey Miner

Publication

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human MYOD1 activating gRNAs #1,2,3 (MYOD1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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LentiU6-mNeurog2 gRNA_1-MS2-Puro

Plasmid

#192680

Purpose

Lentiviral expression of sgRNA targeting mIL1RN promoter to activate mouse Neurog2 transcription

Depositor

Jeffrey Miner

Publication

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Mouse Neurog2 activating gRNA #1 (Neurog2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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LentiU6-hMYOD1 gRNA_1-MS2-Puro

Plasmid

#192673

Purpose

Lentiviral expression of sgRNA targeting hMYOD1 promoter to activate human MYOD1 transcription

Depositor

Jeffrey Miner

Publication

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human MYOD1 activating gRNA #1 (MYOD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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LentiU6-hASCL1 gRNA_1-MS2-Puro

Plasmid

#192670

Purpose

Lentiviral expression of sgRNA targeting hASCL1 promoter to activate human ASCL1 transcription

Depositor

Jeffrey Miner

Publication

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human ASCL1 activating gRNA #1 (ASCL1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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