We narrowed to 5,323 results for: pcDNA3.1

1. (383) pcDNA3.1-HA-nBC2-OGA(544-706)

   Plasmid

   #168100

   Purpose

   HA- tagged BC2 nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA

   Depositor

   Christina Woo

   Article

   Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

   Insert

   HA-nBC2-OGA(544-706)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   OGA truncated to contain residues 544-706

   Promoter

   T7/CMV

   Available Since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3.1-Flag-H2A K13-15-118-119R

   Plasmid

   #63568

   Purpose

   Expression of human H2A (with multiple Lysine to Arginine point mutations) tagged with Flag

   Depositor

   Titia Sixma

   Article

   Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

   Insert

   H2A (H2AC20 Human)
   

   Use

   Tags

   Flag (MDYKDDDDKVPRIQ)

   Expression

   Mammalian

   Mutation

   Lysine at 13, 15, 118, and 119 are now Arginine

   Promoter

   CMV

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1(+)/GGG-nLuc-3XFLAG-CL1/PEST

   Plasmid

   #127321

   Purpose

   Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GGG start codon (Negative control plasmid)

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG and CL1/PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 - PP1g - Flag - BirA* (AA2-140)

   Plasmid

   #86886

   Purpose

   N-terminal part of the split-BioID

   Depositor

   Mathieu Bollen

   Article

   Munter et al FEBS Lett. 2016 Dec 29. doi: 10.1002/187

   Insert

   PP1g - FLAG - BirA (AA2-140)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   BirA Amino Acids 2-140

   Promoter

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3

   Plasmid

   #73205

   Purpose

   Expresses myc-dL5(E52D)-mCer3 fusion protein in nuclei of mammalian cells. (MBIC5, dL5**, FAP)

   Depositor

   Marcel Bruchez

   Article

   Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

   Insert

   NLS-myc-dL5-2XG4S-mCer3 (MYC Synthetic, Human)
   

   Use

   Tags

   The FAP and mCerulean3 are fused with 2 copies of…

   Expression

   Mammalian

   Mutation

   The dL5** FAP is E50D, L89S, also known as E52D/L…

   Promoter

   CMV

   Available Since

   March 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. (302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5)

   Plasmid

   #168192

   Purpose

   HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRs

   Depositor

   Christina Woo

   Article

   Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

   Insert

   HA-LaG6-(EAAAK)4-OGT(4)
   

   Use

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available Since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. (318) pcDNA3.1-myc-OGA(1-400)D174N

   Plasmid

   #168096

   Purpose

   Myc tagged inactive truncated human OGA catalytic domain of residues 1-400 with D174N mutation

   Depositor

   Christina Woo

   Article

   Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

   Insert

   myc-OGA(1-400, D174N)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   D174N catalytically inactive OGA

   Promoter

   T7/CMV

   Available Since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-TAT-1-101-FLAG basic mutant

   Plasmid

   #35985

   Depositor

   Susana Valente

   Article

   Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

   Insert

   transactivating protein (tat )
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   tat from HIV1, 101 aa; R to A mutations at aa 56,…

   Promoter

   Available Since

   Sept. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG M1457A

   Plasmid

   #163988

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 UPA-CARD (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   M1457A

   Promoter

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-CD40L (lose of function mutant)-mgSrtA

    Plasmid

    #125796

    Purpose

    for co-expression of a loss of function mutant of CD40L and mgSrtA on cell membrane. A negative control to plasmid pcDNA3.1-CD40L-mgSrtA.

    Depositor

    P. Chen

    Article

    Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

    Insert

    CD40L (lose of function mutant)-mgSrtA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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