We narrowed to 12,483 results for: BASE

1. pEN_Tmcs

   Plasmid

   #25751

   Purpose

   Entry vector with TRE promoter followed by multiple cloning site.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTS93 pHAGE2 CMVtetO2 GFP-22xGS-MCS

   Plasmid

   #199346

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST26-USP47

   Plasmid

   #64326

   Purpose

   Mammalian expression and purification of USP47.

   Depositor

   Grigory Dianov

   Article

   Parsons et al Mol Cell. 2011 Mar 4;41(5):609-15. doi:

   Insert

   USP47 (USP47 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. AcpP pet29a C-terminal His Tag

   Plasmid

   #75016

   Purpose

   Expresses the acyl carrier protein (ACP) from the E. coli fatty acid synthase

   Depositor

   Michael Burkart

   Article

   Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

   Insert

   AcpP (acpP E. coli)
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 18, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pM201

   Plasmid

   #173179

   Purpose

   dCas9-24xGCN4

   Depositor

   Hajin Kim

   Article

   Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

   Insert

   dCas9-24xGCN4
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pN-PITCh-3XHA

   Plasmid

   #127881

   Purpose

   Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HA tagging

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Insert

   GFP-Puro-3XHA (PPP2CA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pegRNA-GBA-10

   Plasmid

   #199271

   Purpose

   pegRNA used to induce GBA (c. 1226 A > G) mutation

   Depositor

   Ting Zhou

   Article

   Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

   Insert

   GBA 1226AtoG pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. T. aq RNA Polymerase

   Plasmid

   #215349

   Purpose

   Express T. aq RNA Polymerase subunits in E. coli cells

   Depositor

   Julius Lucks

   Article

   Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

   Insert

   RNA polymerase
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Twin-Strep(R)-Tev-GSGS-Ubiquitin

   Plasmid

   #186803

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses Twin-Strep(R)-Tev-GSGS-Ubiquitin.

   Depositor

   Lalit Deshmukh

   Article

   Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

   Insert

   Twin-Strep(R)-Tev-GSGS-Ubiquitin
   

   Use

   Tags

   GS-Strep (WSHPQFER)-GGGSGGGSGGSSA-Strep (WSHPQFER…

   Expression

   Bacterial

   Mutation

   Strep tag Lysine's mutated to Arginine

   Promoter

   Available since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tol2-cmlc2-Trpa1b-2A-EGFP

    Plasmid

    #106405

    Purpose

    For expressing Trpa1b-2A-EGFP in zebrafish cardiomyocytes.

    Depositor

    Randall Peterson

    Article

    Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

    Inserts

    cmlc2 promoter
    Trpa1b (trpa1b Zebrafish)
    

    Use

    Gateway

    Tags

    2A-EGFP

    Expression

    Mutation

    Promoter

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-Sfp R4-4 mutant

    Plasmid

    #173491

    Purpose

    Expression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.

    Depositor

    Jun Yin

    Article

    Sunbul et al J Mol Biol. 2009 Apr 10;387(4):883-98. d

    Insert

    Sfp-R44
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. ZJOM160

    Plasmid

    #133670

    Purpose

    Integration of mTagBFP2-SKL as an additional copy in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast peroxisome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    pTPI1-mTagBFP2-SKL
    

    Use

    Tags

    mTagBFP2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSW002-PpsbA_mTurquoise2

    Plasmid

    #108237

    Purpose

    Broad host bacterial expression vector with constitutive PsbA promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    PsbA promoter - mTurquoise2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNeoXTR f0

    Plasmid

    #69157

    Purpose

    mouse targeting vector

    Depositor

    David Feldser

    Article

    Robles-Oteiza et al Nat Commun. 2015 Nov 5;6:8783. doi: 10.1

    Inserts

    DTA
    NeoR
    GFP
    AmpR
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Amp promoter, PGK, and none

    Available since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. TBP6.7 non-targeting gRNA lambda phage

    Plasmid

    #132547

    Purpose

    expresses gRNA for TBP-based CIRTS

    Depositor

    Bryan Dickinson

    Article

    Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

    Insert

    gRNA TBP-based CIRTS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 promoter

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLPR2

    Plasmid

    #209980

    Purpose

    Contains Level 0 Part: resistance cassette (cat) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    resistance cassette (cat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLPC54

    Plasmid

    #209983

    Purpose

    Contains Level 0* Part: 5' Connector (5C_07_5C1C) for the construction of Level 2 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    5' Connector (5C_07_5C1C)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLPC33

    Plasmid

    #209984

    Purpose

    Contains Level 0* Part: 3' Connector (3C_08_3C3) for the construction of Level 2 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    3' Connector (3C_08_3C3)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLPR3

    Plasmid

    #209986

    Purpose

    Contains Level 0* Part: resistance cassette (ermBL) for the construction of Level 2 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    resistance cassette (ermBL)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLPO5

    Plasmid

    #209987

    Purpose

    Contains Level 0* Part: replication origin (256rep) for the construction of Level 2 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    replication origin (256_rep)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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