We narrowed to 166,040 results for: addgene

1. sgRNA2_GAL4UAS-Luciferase reporter

   Plasmid

   #64158

   Purpose

   Photoactivatable transcription system. Lentiviral expression of sgRNA2 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA2 for GAL4UAS-Luciferase reporter
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. B108-Otx2-IRES-Cre

   Plasmid

   #73993

   Purpose

   B108 enhancer of Blimp1 gene drives Otx2 (isoform b) and Cre expression.

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   B108-Otx2 (isoform b)-IRES-Cre (Otx2 Mouse)
   

   Expression

   Mammalian

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFGC5941-PacI-ath-STTM173

   Plasmid

   #84101

   Purpose

   target miRNA173 for destruction in Arabidopsis thaliana

   Depositor

   Guiliang Tang

   Article

   Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

   Insert

   STTM173
   

   Expression

   Plant

   Promoter

   2x35S

   Available Since

   Nov. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFGC5941-PacI-ath-STTM168

   Plasmid

   #84093

   Purpose

   target miRNA168 for destruction in Arabidopsis thaliana

   Depositor

   Guiliang Tang

   Article

   Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

   Insert

   STTM168
   

   Expression

   Plant

   Promoter

   2x35S

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBP-mTAZ-NFLAG

   Plasmid

   #31791

   Depositor

   Michael Yaffe

   Article

   Hong et al Science. 2005 Aug 12;309(5737):1074-8.

   Insert

   TAZ (Wwtr1 )
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pRRL WS1.6 GFP

   Plasmid

   #36249

   Depositor

   David Rawlings

   Article

   Astrakhan et al Blood. 2012 Mar 19.

   Insert

   GFP (eGFP )
   

   Use

   Lentiviral

   Promoter

   WAS (human 1.6 kb)

   Available Since

   May 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. THI VP64 pGAP T5

   Plasmid

   #161492

   Purpose

   CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T5_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (Targeting T5 in the THI11 promoter)
   

   Expression

   Yeast

   Promoter

   pGAP, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. THI VP64 pGAP T1

   Plasmid

   #161488

   Purpose

   CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T1_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (Targeting T1 in the THI11 promoter)
   

   Expression

   Yeast

   Promoter

   pGAP, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. RCAN1_pCSdest

   Plasmid

   #53883

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   RCAN1 (RCAN1 Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. H2B-CoilE-mEm

    Plasmid

    #181992

    Purpose

    CoilE genetically encoded tag on H2B-mEmerald, to be used with CoilR labeled with a reporter

    Depositor

    Kimberly Beatty

    Article

    Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

    Insert

    H2B-CoilE-mEm
    

    Tags

    mEmerald

    Expression

    Mammalian

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRRG199-bmp4

    Plasmid

    #99076

    Purpose

    dsRNA and riboprobe synthesis for Schmidtea mediterranea Bmp4 (also known as bmp)

    Depositor

    Phillip Newmark

    Article

    Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

    Insert

    Bmp4 (also known as bmp) in situ probe
    

    Use

    T/a cloning vector for dsrna generation and the g…

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7-SpCas9_sgRNA-site2 (RTW448)

    Plasmid

    #160137

    Purpose

    T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Promoter

    T7

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. myc-Rapr-FAK-YM

    Plasmid

    #25927

    Depositor

    Klaus Hahn

    Article

    Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

    Insert

    RapR-FAK (Ptk2 Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    This myc-RapR-FAK-YM plasmid contains FAK mutatio…

    Available Since

    Aug. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

14. MAC_C_TR61B

    Plasmid

    #111679

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    TR61B (TRMT61B Human)
    

    Expression

    Mammalian

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.1 shMTH1-1

    Plasmid

    #21297

    Depositor

    Bob Weinberg

    Article

    Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    shMTH1-1 (NUDT1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    May 29, 2009

    Availability

    Academic Institutions and Nonprofits only

16. N6AMT1_pCSdest

    Plasmid

    #53843

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    N6AMT1 (N6AMT1 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pSLIK 3XFLAG-MKK4-EE-NLS hygro

    Plasmid

    #87779

    Purpose

    Inducible expression of constitutively active mutant MKK4 with a C-terminal NLS sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKK4
    

    Use

    Lentiviral

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pFA6a-kanMX6-Purg1-GFP

    Plasmid

    #39353

    Depositor

    Jurg Bahler

    Article

    Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

    Insert

    urg1 promoter (urg1, urg1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    GFP and kanMX6 module - reverse orientation

    Available Since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

19. sh-RNA hULK2

    Plasmid

    #31970

    Depositor

    Do-Hyung Kim

    Article

    Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

    Insert

    ULK2 (ULK2 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pFGC5941-PacI-ath-STTM5026

    Plasmid

    #84192

    Purpose

    target miRNA5026 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM5026
    

    Expression

    Plant

    Promoter

    2x35S

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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