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Showing: 4161 - 4180 of 65857 results

  9. pMLLCK-J72032

    Plasmid
    #26177
    Depositor
    Christopher Anderson
    Insert
    C terminal S tag
    Use
    Synthetic Biology; 2ab assembly vector
    Available Since
    June 8, 2011
    Availability
    Academic Institutions and Nonprofits only
    26177-plasmid-map-sequence-id-12298

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  12. pcDNA3-Rs4084-RBD-8h

    Plasmid
    #161825
    Purpose
    Mammalian expression plasmid for RBD of bat coronavirus isolate Rs4084
    Depositor
    Erik Procko
    Article
    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf
    Insert
    Receptor-binding domain (RBD) of spike protein S
    Tags
    GSG linker and 8his tag and HA leader sequence
    Expression
    Mammalian
    Mutation
    Human codon optimized RBD (a.a. T321-D519)
    Promoter
    CMV
    Available Since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161825-plasmid-map-sequence-id-316775

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  13. pcDNA3-Rs4231-RBD-8h

    Plasmid
    #161824
    Purpose
    Mammalian expression plasmid for RBD of bat coronavirus isolate Rs4231
    Depositor
    Erik Procko
    Article
    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf
    Insert
    Receptor-binding domain (RBD) of spike protein S
    Tags
    GSG linker and 8his tag and HA leader sequence
    Expression
    Mammalian
    Mutation
    Human codon optimized RBD (a.a. T320-D518)
    Promoter
    CMV
    Available Since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161824-plasmid-map-sequence-id-316774

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  14. pcDNA3-SARS-1-RBD-8h

    Plasmid
    #161821
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-1 (Urbani strain)
    Depositor
    Erik Procko
    Article
    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf
    Insert
    Receptor-binding domain (RBD) of spike protein S
    Tags
    GSG linker and 8his tag and HA leader sequence
    Expression
    Mammalian
    Mutation
    Human codon optimized RBD (a.a. T320-D518)
    Promoter
    CMV
    Available Since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161821-plasmid-map-sequence-id-316749

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  15. pcDNA3.3_SARS2_XBB.1.16

    Plasmid
    #201189
    Purpose
    expressing SARS-CoV-2 XBB.1.16 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (XBB.1.16) (S SARS-CoV-2)
    Expression
    Mammalian
    Mutation
    E180V, T(K)478R on the basis of XBB.1.5
    Promoter
    CMV
    Available Since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    201189-plasmid-map-sequence-id-393640

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  16. FGB

    Plasmid
    #52030
    Purpose
    Expresses full-length Fibrinogen beta chain precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    FGB (FGB Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Stop Codon at amino acid 492, before CD4, bio and…
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52030-plasmid-map-sequence-id-80105

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  17. pEHIShqprt

    Plasmid
    #92166
    Purpose
    Plasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vector
    Depositor
    Huanting Liu, James Naismith
    Article
    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E
    Insert
    Quinolinate Phosphoribosyltransferase (QPRT Human)
    Tags
    TEV protease -cleavable N-terminal 6His
    Expression
    Bacterial
    Mutation
    changed Asp 2 to Asn to create BspHI site
    Promoter
    T7
    Available Since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    92166-plasmid-map-sequence-id-190226

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  19. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid
    #74408
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available Since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74408-plasmid-map-sequence-id-145566

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Showing: 4161 - 4180 of 65857 results