We narrowed to 7,828 results for: CIT

1. pAAV-Syn-Chronos-GFP

   Plasmid

   #59170

   Purpose

   AAV-mediated expression of Chronos-GFP under the Syn promoter

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Has Service

   AAV Retrograde, AAV1, and AAV5

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Syn

   Available since

   Aug. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAG-Chronos-GFP

   Plasmid

   #99232

   Purpose

   AAV-mediated expression of Chronos-GFP under the CAG promoter. Using bGHpA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUltra-Exon1Q23-Myc-B

   Plasmid

   #110488

   Purpose

   Lentiviral vector to express wild-type N terminal HTT gene with partial long 3'UTR in mammalian cells (encodes Myc-tagged wild-type huntingtin exon1 fragment with 23 repeats of Q)

   Depositor

   Baoji Xu

   Article

   Xu et al PLoS One. 2017 May 11;12(5):e0177610. do

   Insert

   Homo sapiens huntingtin (HTT Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   short 3'UTR mutant huntingtin exon1 fragment…

   Promoter

   UbC

   Available since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. CamKII-LGI1-pHluorin

   Plasmid

   #185537

   Purpose

   Expresses LGI1-pHluorin under the CamKII promoter

   Depositor

   Jaime de Juan-Sanz

   Article

   Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

   Insert

   LGI1 (Lgi1 Rat)
   

   Use

   Lentiviral

   Tags

   pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   CamKII

   Available since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. LV_EF1a_CDK1(T14A,Y15F)-P2A-Hygro_Barcode

   Plasmid

   #170220

   Purpose

   Barcoded lentiviral vector to express CDK1 (T14A, Y15F) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   CDK1 (CDK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   T14A, Y15F

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ai66(RCRL-tdT) targeting vector

   Plasmid

   #61578

   Purpose

   Target a Dre and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   CAG-RSR-LSL-tdTomato
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUltra-Exon1Q145-Myc-B

   Plasmid

   #110489

   Purpose

   Lentiviral vector to express mutant N terminal HTT gene with partial long 3'UTR in mammalian cells (encodes Myc-tagged mutant huntingtin exon1 fragment with 145 repeats of Q)

   Depositor

   Baoji Xu

   Article

   Xu et al PLoS One. 2017 May 11;12(5):e0177610. do

   Insert

   Homo sapiens huntingtin (HTT Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   partial long 3'UTR wild type huntingtin exon…

   Promoter

   UbC

   Available since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV pTRE-FLEX-EGFP-WPRE-bGHpA

   Plasmid

   #65449

   Purpose

   Can be used to generate AAV virus that will express EGFP from the tetO promoter in a Cre-dependent manner

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   tetO

   Available since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX6P2-p53 (1-90) (72R)

   Plasmid

   #62081

   Purpose

   Expression of human p53 (TAD residues 1-90) (72R) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Contains TAD/PP residues 1-90, 72R

   Promoter

   tac

   Available since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pCMV-HF-BE3

    Plasmid

    #87439

    Purpose

    Expresses HF-BE3 with C-terminal NLS in mammalian cells

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

    Insert

    HF-BE3 (Apobec1 Bacillus phage PBS2, Rat, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mammalian codon-optimized Cas9-HF

    Promoter

    CMV

    Available since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CaMKII-Chrimson-GFP

    Plasmid

    #62718

    Purpose

    AAV-mediated expression of Chrimson-GFP under the CaMKII promoter. Using SV40pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chrimson-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII

    Available since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRGEN-CMV-10XGCN4-L1-dCjCas9 S-D

    Plasmid

    #169912

    Purpose

    Expression of a SunTag-dCjCas9 fusion protein in which 10 copies of the GCN4 peptide are fused to the N terminus of dCjCas9.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Insert

    dCjCas9
    

    Use

    CRISPR

    Tags

    10XGCN4, 2xSV40NLS, and SV40NLS-HA

    Expression

    Mammalian

    Mutation

    D8A, H559A in cjCas9

    Promoter

    CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EF1α1.1-tdTomato

    Plasmid

    #122101

    Purpose

    AAV-mediated expression of tdTomato under the EF1α promoter (1.1kb short version). tdTomato has codons varied to reduce recombination. Using SV40 pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    tdTomato
    

    Use

    AAV

    Tags

    NA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a（1.1kb short version)

    Available since

    June 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTE4497

    Plasmid

    #80339

    Purpose

    mammalian expression FnCpf1 nuclease and Fn crRNA

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Fn crRNA
    FnCpf1
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and human U6

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-FLEX-fwd[Chrimson-tdT]

    Plasmid

    #59137

    Purpose

    Expression vector for Chrimson-tdTomato in a floxed/forward-reading, Cre-dependent manner (Cre turns gene off)

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chrimson-tdTomato
    

    Use

    Cre/Lox

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-AKAP79-(Ci/Ce)-Epac2-camps

    Plasmid

    #181956

    Purpose

    Genetically encoded FRET-based cAMP indicator fused to AKAP79.

    Depositor

    Jin Zhang

    Article

    Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

    Insert

    AKAP79-(Ci/Ce)-Epac2-camps (AKAP5 Human)
    

    Use

    Tags

    AKAP79, Cerulean (CFP), Citrine (YFP), and HIS ta…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX6P2-MDMX (23-111)

    Plasmid

    #62064

    Purpose

    Expression of human MDM4 (residues 23-111) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    MDM4 (MDM4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains residues 23-111

    Promoter

    tac

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

18. LV_EF1a_MEK1(S218D,S222D)-P2A-Hygro_Barcode

    Plasmid

    #170240

    Purpose

    Barcoded lentiviral vector to express MEK1 (S218D, S222D) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S218D, S222D

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EF1α1.1-GFP

    Plasmid

    #122100

    Purpose

    AAV-mediated expression of GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    GFP
    

    Use

    AAV

    Tags

    NA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a（1.1kb short version)

    Available since

    May 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CMV-ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-RhoA

    Plasmid

    #165437

    Purpose

    Expresses ShadowY-CBD(H83L/H86L) and Clover(T153M/F223R)-RhoA in mammalian cells as a negative control for FLIM imaging.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-RhoA
    

    Use

    Tags

    ShadowY, Clover(T153M/F223R)

    Expression

    Mammalian

    Mutation

    H83L/H86L: CDB(Cdc42 Binding Domain, Pak3, residu…

    Promoter

    CMV

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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