Addgene

We narrowed to 788 results for: Nf1

Showing: 601 - 630 of 788 results

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pHH0103_SH3RF1-1/4

Plasmid

#91544

Purpose

Protein expression and purification of human SH3 domain construct SH3RF1-1/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF1-1/4 (SH3RF1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF1-2/4

Plasmid

#91457

Purpose

Protein expression and purification of human SH3 domain construct SH3RF1-2/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF1-2/4 (SH3RF1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF1-4/4

Plasmid

#91374

Purpose

Protein expression and purification of human SH3 domain construct SH3RF1-4/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF1-4/4 (SH3RF1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF1-3/4

Plasmid

#91366

Purpose

Protein expression and purification of human SH3 domain construct SH3RF1-3/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF1-3/4 (SH3RF1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEBB-3XMyc-TRAF2 S274A

Plasmid

#44107

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S274A (TRAF2 Human)

Use

Tags

3xMyc

Expression

Mammalian

Mutation

Serine 274 to Alanine

Promoter

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
HA-RIPLET ΔPRY/SPRY

Plasmid

#218516

Purpose

RIPLET lacking C-terminal PRY/SPRY domain in pcDNA3.1

Depositor

Sun Hur

Article

Cadena et al Cell. 2019 May 16;177(5):1187-1200.e16.

Insert

RIPLET delta PRY/SPRY domain (RNF135 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 11, 2024

Availability

Academic Institutions and Nonprofits only

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AA091

Plasmid

#215979

Purpose

Fragmid fragment: (N' terminus) ZNF10 KRAB

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; BPNLS_v1.1; KRAB_v1.1

Use

Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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MCP-mCherry-TIS11B

Plasmid

#207670

Purpose

To express fluorescently labeled MS2 coat binding protein (MCP) fused with RNA binding protein TIS11B

Depositor

Christine Mayr

Article

Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

Insert

TIS11B (ZFP36L1 Human)

Use

Tags

MCP-mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIP-GFP-BAF

Plasmid

#164248

Purpose

retroviral plasmid for GFP-BAF

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

BAF (BANF1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEBB-3XMyc-TRAF2 S327A

Plasmid

#44108

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S327A (TRAF2 Human)

Use

Tags

Expression

Mammalian

Mutation

Serine 327 to Alanine

Promoter

EF1α

Available since

May 2, 2013

Availability

Academic Institutions and Nonprofits only
pRDB_181

Plasmid

#216102

Purpose

CRISPRi, EF1a-driven dCas9-KRAB (ZNF10) (Cas only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas9 [Sp]

Use

CRISPR and Lentiviral; Assembled vector

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRDA_662

Plasmid

#216101

Purpose

CRISPRi, EFS-driven dCas9-KRAB (ZNF10) (Cas only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas9 [Sp]

Use

CRISPR and Lentiviral; Assembled vector

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-GFI1

Plasmid

#16168

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Growth factor independent 1 (GFI1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pETM33_TNKS_TANK-peptide-bind

Plasmid

#178489

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

TNKS_TANK-peptide-bind (TNKS Human, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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LTN1_pCSdest

Plasmid

#53855

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

LTN1 (LTN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

Sept. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR-GFI1B

Plasmid

#16169

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Growth factor independent 1B (GFI1B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pHH0103_SH3RF2-3/3

Plasmid

#91281

Purpose

Protein expression and purification of human SH3 domain construct SH3RF2-3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF2-3/3 (SH3RF2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF2-2/3

Plasmid

#91557

Purpose

Protein expression and purification of human SH3 domain construct SH3RF2-2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF2-2/3 (SH3RF2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_SH3RF2-1/3-SV2/2

Plasmid

#91408

Purpose

Protein expression and purification of human SH3 domain construct SH3RF2-1/3-SV2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF2-1/3-SV2/2 (SH3RF2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF2-1/3-SV1/2

Plasmid

#91322

Purpose

Protein expression and purification of human SH3 domain construct SH3RF2-1/3-SV1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF2-1/3-SV1/2 (SH3RF2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-Neurofascin (extracellular) [A12/18R] (Recombinant Antibody)

Antibody

#184174-rAb

Purpose

Anti-Pan-Neurofascin (extracellular) (Rat) recombinant mouse monoclonal antibody

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Recommended Applications

Immunohistochemistry and Western Blot

Reactivity

Human and Rat

Source Species

Mouse

Isotype

IgG2a

Trial Size

Available to purchase

Available since

June 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits
pFUW-TetO-ZFP36

Plasmid

#178438

Purpose

doxycycline-inducible expression of Human ZFP36 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

ZFP36 (ZFP36 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-BAF

Plasmid

#227442

Purpose

Mammalian expression of GFP-BAF

Depositor

David Pellman

Article

Umbreit et al Science. 2020 Apr 17;368(6488):eaba0712.

Insert

Barrier-to-autointegration factor (BANF1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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SNAP-BAF

Plasmid

#227443

Purpose

Mammalian expression of SNAP-BAF

Depositor

David Pellman

Article

Umbreit et al Science. 2020 Apr 17;368(6488):eaba0712.

Insert

Barrier-to-autointegration factor (BANF1 Human)

Use

Lentiviral

Tags

SNAP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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SID4x-dCas9-KRAB

Plasmid

#106399

Purpose

Expresses catalytically inactive dCas9 with N-terminal fusion of Sin3a Interacting Domain (SID) and C-terminal fusion of repressive KRAB domain

Depositor

Jason Gertz

Article

Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

Inserts

SID4x (MXD1 Human)
KRAB (ZNF10 Human)

Use

Tags

FLAG, HA, and NLS

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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ZFP36L2

Plasmid

#156149

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

ZFP36L2 (ZFP36L2 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-PLZF

Plasmid

#61543

Purpose

Expresses PLZF in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

PLZF (ZBTB16 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

I380T and G580D

Promoter

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only

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TFORF3150

Plasmid

#144626

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF254 (ZNF254 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF1761

Plasmid

#142843

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF24 (ZNF24 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2753

Plasmid

#144229

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF445 (ZNF445 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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