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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 935 results for: RK2;

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Showing: 401 - 420 of 935 results

  1. SAGA039

    Plasmid
    #240788
    Purpose
    Plasmid contains the triple selection cassette gfp -RK2 tetARK2 - CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA RK2 His low : T7- His tags low - gfp -RK2 tetARK2 - CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240788-plasmid-map-sequence-id-484210

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  3. pOGG010

    Plasmid
    #113984
    Purpose
    Vector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectors
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    RK2 (IncP)
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    113984-plasmid-map-sequence-id-223395

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  11. pRPCN

    Plasmid
    #193726
    Purpose
    Expresses Cas9 from RK2 origin with RFP-marked sgRNA insertion site and kanR/sacB selection/counterselection
    Depositor
    Martin Buck
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Available Since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    193726-plasmid-map-sequence-id-381907

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  12. Escherichia coli DH10B-MOB

    Bacterial Strain
    #229395
    Purpose
    Escherichia coli DH10B DAP-auxotrophic strain containing an integrated RK2/RP4 mobilization vector to conjugate plasmids with an IncP origin of transfer (modified pTA-Mob 2.0, AddGene Plasmid #149662)
    Depositor
    Michael Springer
    Article
    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1
    Bacterial Resistance
    60 ug/mL of DAP
    Species
    Escherichia coli
    Available Since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only

  13. pRPCE

    Plasmid
    #193712
    Purpose
    Expresses cytosine deaminase linked deactivated-Cas9 from RK2 origin with RFP-marked sgRNA insertion site and kanR/sacB selection/counterselection
    Depositor
    Martin Buck
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Available Since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    193712-plasmid-map-sequence-id-381728

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  14. pHflu1

    Plasmid
    #203878
    Purpose
    pSU20 with addition of CEN6-ARSH4-HIS3 cassette for S. cerevisiae and RK2 origin of conjugal transfer
    Depositor
    Bogumil Karas
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    203878-plasmid-map-sequence-id-408184

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Showing: 401 - 420 of 935 results