Addgene

We narrowed to 623 results for: SPEN

Showing: 401 - 420 of 623 results

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B*40:01:01

Plasmid

#203257

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*40:01:01 (HLA-B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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B*58:01:01

Plasmid

#203261

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*58:01:01 (HLA-B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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J23104-mRFP1-331Bb

Plasmid

#78274

Purpose

mRFP1 behind constitutive promoter J23104 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23104-mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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A*02:02:01:01

Plasmid

#203277

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*02:02:01:01 (HLA-A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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A*11:01:01:01

Plasmid

#203276

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*11:01:01:01 (HLA-A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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A*33:01:01

Plasmid

#203288

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*33:01:01 (HLA-A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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J-sRNA-331Bb

Plasmid

#78285

Purpose

An inducible small RNA with E.coli Hfq targeting K. rhaeticus UGPase mRNA

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

j-sRNA-331

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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B*58:02:01

Plasmid

#203262

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*58:02:01 (HLA-B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
A*66:01:01

Plasmid

#203291

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*66:01:01 (HLA-A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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A*03:02:01

Plasmid

#203294

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*03:02:01 (HLA-A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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Human FLAG-WDR20

Plasmid

#217618

Purpose

Mammalian expression of FLAG tagged WDR20

Depositor

Ethan Lee

Article

Ng et al Nat Commun. 2023 Oct 5;14(1):6173. doi:

Insert

WDR20 (WDR20 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD4 (OTU, aa 22-196)

Plasmid

#61413

Purpose

Expresses human OTUD4 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD4 (OTU domain-containing protein 4) (OTUD4 Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-21 and aa 197-1114.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINK-Cezanne2 (OTU+UBA, aa 1-462)

Plasmid

#61582

Purpose

Expresses human Cezanne2 (OTU+UBA domains) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

Cezanne2 (OTUD7A Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Isoform 2. Deleted aa 463-933.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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A*01:03:01

Plasmid

#203293

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*01:03:01 (HLA-A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pRM27

Plasmid

#163695

Purpose

ubb promoter driven expression of lck:mNeonGreen

Depositor

David Matus

Article

Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

Insert

lck:mNeonGreen (lck Zebrafish)

Use

Zebrafish expression

Tags

mNeonGreen

Expression

Mutation

Promoter

ubb

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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mApple-Vinculin-23

Plasmid

#54961

Purpose

Localization: Focal Adhesions, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Article

Thievessen et al J Cell Biol. 2013 Jul 8;202(1):163-77. d

Insert

Vinculin (VCL Human)

Use

Tags

mApple

Expression

Mammalian

Mutation

V234L in Vinculin.

Promoter

CMV

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
pNA0304

Plasmid

#165612

Purpose

Vector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)

Depositor

Marcus Noyes

Article

Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

Insert

SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)

Use

CRISPR

Tags

Expression

Yeast

Mutation

NotI site replaced the CAN1 spacer in parent vect…

Promoter

SNR52

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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J23117-mRFP1-331Bb

Plasmid

#78280

Purpose

mRFP1 behind constitutive promoter J23117 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23117-mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pGG426_UV5

Plasmid

#165608

Purpose

Vector for expression of the SpCas9 VRKG variant with sgRNA in E. coli: lacUV5-VRKG(SpCas9, D1135V/S1136R/D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)

Depositor

Marcus Noyes

Article

Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

Insert

lacUV5 driving Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G) and hEGFP-sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

D1135V, S1136R, D1332K and R1333G mutations in Sp…

Promoter

lacUV5 driving Cas9 VRKG and UV5 driving sgRNA

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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B*07:05:01

Plasmid

#203268

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*07:05:01 (HLA-B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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