We narrowed to 901 results for: golden gate assembly
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Plasmid#194997Purposeterminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T1 position (E/F)DepositorInsert(E) tNOS (F)
ExpressionBacterialAvailable SinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
(B) BLE marker(R) (SBE262)
Plasmid#195012Purposebleomycin (BLE) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPDDepositorInsertpXYL+BLE+tGPD
ExpressionBacterialPromoterpXYLAvailable SinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
(R)pGPD1(D)_P1(SBE109)
Plasmid#194993Purposepromoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P1 position (overhangs R/D)DepositorInsert(R) pGPD (D)
ExpressionBacterialAvailable SinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
(F)pGPD(G)_P2 (SBE110)
Plasmid#194992Purposepromoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P2 position (overhangs F/G)DepositorInsert(F) pGPD (G)
ExpressionBacterialAvailable SinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
(I) pADH2 (J) P3 (SBE114)
Plasmid#187629Purposepromoter of the gene alcohol dehydrogenase 2 from R. toruloides for the P3 position (I/J)DepositorInsert(I) pADH2 (J)
ExpressionBacterialAvailable SinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
(F) pADH2 (G)_P2 (SBE113)
Plasmid#187628Purposepromoter of the gene alcohol dehydrogenase 2 from R. toruloides fro the P2 position (F/G)DepositorInsert(F) pADH2 (G)
ExpressionBacterialAvailable SinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
ku70 insUP+pLDP1+NAT+tNOS+ku70 insD (SBE193)
Plasmid#195054Purposeintegration cassette for ku70 deletion and expression of NAT gene under pLDP1 for characterizationDepositorInsertNAT
ExpressionBacterialPromoterpLDP1Available SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
ku70 insUP+pFBA+NAT+tNOS+ku70 insD (SBE179)
Plasmid#195053Purposeintegration cassette for ku70 deletion and expression of NAT gene under pFBA for characterizationDepositorInsertNAT
ExpressionBacterialPromoterpFBAAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
ku70 insUP+pXYL+NAT+tNOS+ku70 insD (SBE178)
Plasmid#195052Purposeintegration cassette for ku70 deletion and expression of NAT gene under pXYL for characterizationDepositorInsertNAT
ExpressionBacterialPromoterpXYLAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
ku70 insUP+pGPD+NAT+tNOS+ku70 insD (SBE175)
Plasmid#195051Purposeintegration cassette for ku70 deletion and expression of NAT gene under pGPD for characterizationDepositorInsertNAT
ExpressionBacterialPromoterpGPDAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
ku70 insUP+pADH2+NAT+tNOS+ku70 insD (SBE174)
Plasmid#195050Purposeintegration cassette for ku70 deletion and expression of NAT gene under pADH2 for characterizationDepositorInsertNAT
ExpressionBacterialPromoterpADH2Available SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(F) pXYL (G) P1 (SBE216)
Plasmid#195017Purposepromoter of the gene xylose reductase from R. toruloides for the position P2 (F/G)DepositorInsert(F) pXYL (G)
ExpressionBacterialAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(R) pXYL (D) P1 (SBE215)
Plasmid#195016Purposepromoter of the gene xylose reductase from R. toruloides for the position P1 (R/D)DepositorInsert(R) pXYL (D)
ExpressionBacterialAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(R)pADH2(D)_P1(SBE214)
Plasmid#195015Purposepromoter of the gene alcohol dehydrogenase 2 from R. toruloides for the position P1DepositorInsert(B) pADH2 (R)
ExpressionBacterialAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(B) NAT marker (R) (SBE173)
Plasmid#195014Purposenourseothricin (NAT) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOSDepositorInsertpXYL+NAT+tNOS
ExpressionBacterialPromoterpXYLAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(I) pGPD (J)_P3 (SBE111)
Plasmid#194990Purposepromoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P3 position (I/J)DepositorInsert(I) pGPD (J)
ExpressionBacterialAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(S) insD_ku70 (Q) (SBE139)
Plasmid#187627Purposethe insertional region downstream for the deletion of the KU70 gene in R. toruloides. Overhangs S/QDepositorInsert(S) insD KU70 (Q)
ExpressionBacterialAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(P) insUP_ku70 (SBE138) (B)
Plasmid#187626Purposethe insertional region upstream for the deletion of the KU70 gene in R. toruloides. Overhangs P/BDepositorInsert(P) insUP KU70 (B)
ExpressionBacterialAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Yarrowia lipolytica Golden Gate tool kit
Plasmid Kit#1000000167PurposeYarrowia lipolytica Golden Gate tool kit for integrative vector assembly of up to three transcription units in the oleaginous yeast Y. lipolytica.DepositorAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
GGDestX1-Amp
Plasmid#157652PurposeGolden Gate assembly and cell-free (bacterial) expression of fusion-proteinsDepositorTypeEmpty backboneUseCell-free expression (e. coli sigma-70)ExpressionBacterialPromoterOR2-OR1-PrAvailable SinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only