We narrowed to 860 results for: golden gate assembly

1. (B) NAT marker (R) (SBE173)

   Plasmid

   #195014

   Purpose

   nourseothricin (NAT) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOS

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   pXYL+NAT+tNOS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pXYL

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. (I) pGPD (J)_P3 (SBE111)

   Plasmid

   #194990

   Purpose

   promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P3 position (I/J)

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (I) pGPD (J)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. (S) insD_ku70 (Q) (SBE139)

   Plasmid

   #187627

   Purpose

   the insertional region downstream for the deletion of the KU70 gene in R. toruloides. Overhangs S/Q

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (S) insD KU70 (Q)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. (P) insUP_ku70 (SBE138) (B)

   Plasmid

   #187626

   Purpose

   the insertional region upstream for the deletion of the KU70 gene in R. toruloides. Overhangs P/B

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (P) insUP KU70 (B)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GGDestX1-Amp

   Plasmid

   #157652

   Purpose

   Golden Gate assembly and cell-free (bacterial) expression of fusion-proteins

   Depositor

   Karmella Haynes

   Article

   Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

   Type

   Empty backbone

   Use

   Cell-free expression (e. coli sigma-70)

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   OR2-OR1-Pr

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GGDestX2-Amp

   Plasmid

   #157654

   Purpose

   Golden Gate assembly and mammalian expression of dCas9 fusion-proteins plus gRNA

   Depositor

   Karmella Haynes

   Article

   Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. C77m

   Plasmid

   #120989

   Purpose

   MoClo golden gate assembly CD partfor AmpR (ampicillin/carbenicillin resistance protein). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   AmpR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. C78m

   Plasmid

   #120990

   Purpose

   MoClo golden gate assembly CD cloning part for ChlorR (chloramphenicol resistance protein). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   ChlorR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. C80m

   Plasmid

   #120992

   Purpose

   MoClo golden gate assembly CD part for mfLon protease (see doi: [10.1038/nbt.3053]). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   mfLon protease
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. C86m

    Plasmid

    #120997

    Purpose

    MoClo golden gate assembly CD part (phage lysis protein; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    X174E
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. C11m

    Plasmid

    #120959

    Purpose

    MoClo golden gate assembly CD part for Citrine (modified YFP; variant of https://www.fpbase.org/protein/citrine/) - Please see Supplemental Documents for annotated Genbank file

    Depositor

    Richard Murray

    Insert

    Citrine
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. C5m

    Plasmid

    #120957

    Purpose

    MoClo golden gate assembly CD part for mRFP (monomeric red fluorescent protein; see https://doi.org/10.1073/pnas.082243699 and https://www.fpbase.org/protein/mrfp1/).

    Depositor

    Richard Murray

    Insert

    mRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. C115m

    Plasmid

    #121014

    Purpose

    MoClo golden gate assembly CD part for TP901 integrase (Integrase from lactococcal phage TP901). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    TP901 integrase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. U22m

    Plasmid

    #121025

    Purpose

    MoClo golden gate assembly BC part (high expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).

    Depositor

    Richard Murray

    Insert

    BCD6
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. C32m

    Plasmid

    #120967

    Purpose

    MoClo golden gate assembly CD part for CinR (Quorum-sensing receptor for 3OC14). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    ohCD carb CinR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. C36m

    Plasmid

    #120970

    Purpose

    MoClo golden gate assembly CD part for RhlI (quorum-sensing synthase for 3OC14). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    ohCD carb RhlI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. C40m

    Plasmid

    #120973

    Purpose

    MoClo golden gate assembly CD part for blank part (random 50bp sequence) - Please see Supplemental Documents for annotated Genbank file

    Depositor

    Richard Murray

    Insert

    Blank (Random 50bp sequence)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. C59m

    Plasmid

    #120976

    Purpose

    MoClo golden gate assembly CD part for mScarlet (RFP variant; see https://www.fpbase.org/protein/mscarlet/). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    mScarlet
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. C13m

    Plasmid

    #120960

    Purpose

    MoClo golden gate assembly CD part for Cerulean (modified CFP; variant of https://www.fpbase.org/protein/cerulean/) - Please see Supplemental Documents for annotated Genbank file

    Depositor

    Richard Murray

    Insert

    Cerulean
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. P1m

    Plasmid

    #120934

    Purpose

    MoClo golden gate assembly AB part plasmid for J23101 (strength 1791x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson); AB overhangs.

    Depositor

    Richard Murray

    Insert

    J23101
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge