We narrowed to 1,800 results for: plasmid for e coli

1. pBEL2200

   Plasmid

   #170407

   Purpose

   Genetic complementation plasmid of LetA-[LetB PLL6->3] mutant

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Insert

   LetAB (letB, letA E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetA-LetB(Δ702-716, replace with 347-360)

   Promoter

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBEL2201

   Plasmid

   #170408

   Purpose

   Genetic complementation plasmid of LetA-[LetB PLL6->5] mutant

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Insert

   LetAB (letB, letA E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetA-LetB(Δ702-716, replace with 580-600)

   Promoter

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBEL2254

   Plasmid

   #170409

   Purpose

   Genetic complementation plasmid of LetA-[LetB PLL6->2] mutant

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Insert

   LetAB (letB, letA E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetA-LetB(Δ700-717, replace with 225-249)

   Promoter

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBEL2255

   Plasmid

   #170410

   Purpose

   Genetic complementation plasmid of LetA-[LetB PLL6->4] mutant

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Insert

   LetAB (letB, letA E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetA-LetB(Δ700-716, replace with 445-479)

   Promoter

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBEL2256

   Plasmid

   #170411

   Purpose

   Genetic complementation plasmid of LetA-[LetB PLL6->7] mutant

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Insert

   LetAB (letB, letA E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetA-LetB(Δ700-717, replace with 812-836)

   Promoter

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBEL2199

   Plasmid

   #170406

   Purpose

   Genetic complementation plasmid of LetA-[LetB PLL6->1] mutant

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Insert

   LetAB (letB, letA E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetA-LetB(Δ702-717, replace with 114-130)

   Promoter

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHelper_O

   Plasmid

   #173783

   Purpose

   The 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.

   Depositor

   Keith Shearwin

   Article

   Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

   Insert

   186 Integrase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   E59G - please see depositor comments

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHelper_W

   Plasmid

   #173784

   Purpose

   The Wphi helper plasmid necessary for genomic integration of the pIT5_KW plasmid.

   Depositor

   Keith Shearwin

   Article

   Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

   Insert

   Wphi Integrase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHelper_D

   Plasmid

   #173781

   Purpose

   The D145 helper plasmid necessary for genomic integration of the pIT5_KD plasmid.

   Depositor

   Keith Shearwin

   Article

   Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

   Insert

   D145 Integrase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FruR-pHG165a (CRA)

    Plasmid

    #90066

    Purpose

    full length WT FruR (CRA)

    Depositor

    Liskin Swint-Kruse

    Insert

    FruR (CRA)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Iq

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pE_Cre_lambdaCos

    Plasmid

    #173785

    Purpose

    The pE_Cre_lambdaCos plasmid is used to remove the antibiotic resistance cassette from pIT5 integrants.

    Depositor

    Keith Shearwin

    Article

    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

    Insert

    Cre
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mutation

    S102Y and S279Y- please see depositor comments

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pORTMAGE502B

    Plasmid

    #128971

    Purpose

    Broad-host-range GenR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid induction

    Depositor

    George Church

    Inserts

    PapRecT
    mutL E32K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E32K

    Promoter

    xyl-Pm

    Available since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pORTMAGE302A

    Plasmid

    #128970

    Purpose

    Broad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with high temperature induction

    Depositor

    George Church

    Inserts

    PapRecT
    mutL E32K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E32K

    Promoter

    cI-pL

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLNKR1

    Plasmid

    #67968

    Purpose

    High copy number plasmid encoding part of the tra Operon PY-traR

    Depositor

    Joshua Leonard

    Insert

    tra Operon (First Quarter)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLNKR2

    Plasmid

    #67969

    Purpose

    High copy number plasmid encoding part of the tra Operon traC-artA

    Depositor

    Joshua Leonard

    Insert

    tra Operon (second Quarter)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pACYC-LIC+

    Plasmid

    #62312

    Purpose

    E. coli pACYC based expression vector with N-terminal Hexa-HIS and a thrombin protease cleavage site

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHis and thrombin protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pACYC-GST

    Plasmid

    #62329

    Purpose

    E. coli pACYC based expression vector with N-terminal TEV cleavable GST-tag

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    Glutathione S-transferase (GST) tag and TEV prote…

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lac0

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET32a-LIC

    Plasmid

    #62310

    Purpose

    E. coli expression vector with N-terminal Trx-tag, 6X His-tag, thrombin site, S-tag, and TEV

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHis, S-tag, and Trx-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBad-HPII

    Plasmid

    #105839

    Purpose

    expresses E. coli HPII catalase with N-terminal 6x his tag

    Depositor

    Ryan Mehl

    Article

    Mehl et al 2020

    Insert

    hydroperoxidase II catalase
    

    Use

    Tags

    6x his tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBad/arabinose

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSB1C3_YPet-PCP (y-plasmid)

    Plasmid

    #127637

    Purpose

    Encodes a fluorecent protein with an RNA binding peptide: YPet-PCP.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme RiboJ and RBS BBa_B0034.

    Depositor

    Friedrich Simmel

    Article

    Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.

    Insert

    YPet
    

    Use

    Synthetic Biology

    Tags

    RNA binding peptide: PP7 coat protein (PCP)

    Expression

    Bacterial

    Mutation

    Promoter

    pJ21306

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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