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pcDNA3.1(+)_Granulin 6-no linker

Plasmid

#176929

Purpose

express Granulin 6 without linker 7 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 6 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Granulin 6+linker7

Plasmid

#176928

Purpose

express Granulin 6 with linker 7 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 6+linker7 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Granulin 5-no linker

Plasmid

#176927

Purpose

express Granulin 5 without linker 6 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 5 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Granulin 5+linker6

Plasmid

#176926

Purpose

express Granulin 5 with linker 6 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 5+linker6 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Granulin 3-no linker

Plasmid

#176923

Purpose

express Granulin 3 without linker 4 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 3 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Granulin 3+linker4

Plasmid

#176922

Purpose

express Granulin 3 with linker 4 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 3+linker4 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Granulin 1-no linker

Plasmid

#176919

Purpose

express Granulin 1 without linker 2 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 1 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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FKBP-EGFP-RanGAP*

Plasmid

#175238

Purpose

Bacterial expression and purification, low affinity SUMOylation substrate that can recruited to FRB with rapamycin, point mutation F562A reduces affinity for E2, increasing the Km

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

RANGAP1 (RANGAP1 Human)

Expression

Bacterial

Mutation

Amino acid 562 F to A

Promoter

tac

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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FKBP-EGFP-RanGAP

Plasmid

#175236

Purpose

Bacterial expression and purification, high affinity SUMOylation substrate that can recruited to FRB with rapamycin

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

RANGAP1 (RANGAP1 Human)

Expression

Bacterial

Promoter

tac

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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C122-E15: mOrange

Plasmid

#162895

Purpose

Standard Gateway entry clone encoding mOrange fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Orange fluorescent protein

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAS2-his-24::mOrange

Plasmid

#124326

Purpose

Expressing Nuclear Localized mOrange

Depositor

Andrew Leifer

Insert

mOrange (his-24 Nematode)

Expression

Worm

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pAS1-his-24::mOrange

Plasmid

#124325

Purpose

Expressing Nuclear Localized mOrange

Depositor

Andrew Leifer

Insert

mOrange (his-24 Nematode)

Expression

Worm

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-M-RAN-H2A2B

Plasmid

#106420

Purpose

M-RAN-H2A2B (RANbody H2A2B::Sm-MYCtag)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

M-RAN-H2A2B

Tags

HA, His, and Multiple MYC

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Y-RAN-H2A2B

Plasmid

#106421

Purpose

Y-RAN-H2A2B (RANbody H2A2B::Chicken IgY)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

Y-RAN-H2A2B

Tags

HA and His

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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RANGAP1 G6.4 gRNA

Plasmid

#90873

Purpose

3rd generation lentiviral gRNA plasmid targeting human RANGAP1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

RANGAP1 (Guide Designation G6.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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RANGAP1 G5.4 gRNA

Plasmid

#90872

Purpose

3rd generation lentiviral gRNA plasmid targeting human RANGAP1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

RANGAP1 (Guide Designation G5.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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RAN G8.4 gRNA

Plasmid

#90871

Purpose

3rd generation lentiviral gRNA plasmid targeting human RAN

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

RAN (Guide Designation G8.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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RAN G7.4 gRNA

Plasmid

#90870

Purpose

3rd generation lentiviral gRNA plasmid targeting human RAN

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

RAN (Guide Designation G7.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCIneoRL-RanBP9 3'UTR

Plasmid

#84591

Purpose

Renilla luciferase reporter containing RANBP9 3' UTR

Depositor

Yanan Yang

Article

Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

Insert

RANBP9 3'UTR (RANBP9 Human)

Use

Luciferase

Tags

Renilla Luciferase

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only
CpcB-Abies-grandisPHLS_CpcA

Plasmid

#74000

Purpose

Expresses the Abies grandis PHLS as a fusion with the CpcB protein plus the CmR cassete in Synechocystis

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2018 Oct;248(4):933-946. doi: 10

Insert

PHLS and CmR

Promoter

cpc

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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