We narrowed to 5,172 results for: PEP

1. pET28b-hTIG3-1-132

   Plasmid

   #100720

   Purpose

   Expression of N-terminal region of human TIG3 in E.coli

   Depositor

   Byung Il Lee

   Article

   Han et al Protein Expr Purif. 2010 May;71(1):103-7

   Insert

   RARRES3 (PLAAT4 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promotor

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCerulean3-ER-5

   Plasmid

   #55418

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Insert

   ER (CALR Human)
   

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   ER Signal Peptide

   Promoter

   CMV

   Available since

   Dec. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pYO359

   Plasmid

   #235750

   Purpose

   Protein expression of FL ScVPS34 and FL ScVPS15-ZZ

   Depositor

   Roger Williams

   Article

   Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

   Inserts

   VPS34 (VPS34 Budding Yeast)
   VPS15 (VPS15 Budding Yeast)
   

   Use

   Tags

   3xTEV-ZZ

   Expression

   Yeast

   Mutation

   S2A, aa 3-887 and T134A, I851R

   Promoter

   GAL-TDH3

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLuc-C-NLuc

   Plasmid

   #234566

   Purpose

   Bacterially expressed FLuc-C-NLuc for Ni-NTA purification

   Depositor

   James Inglese

   Article

   Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

   Insert

   Firefly luciferase
   

   Use

   Tags

   NanoLuciferase-thrombin site-10xHis and T7 gene 1…

   Expression

   Bacterial

   Mutation

   Codon optmization for expression in BL21DE3

   Promoter

   T7

   Available since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-hPLEKHA6-Myc

   Plasmid

   #236540

   Purpose

   For expression of PLEKHA6 (human) in mammalian cells

   Depositor

   Sandra Citi

   Article

   Sluysmans et al Mol Biol Cell. 2021 Nov 1;32(21):ar34. d

   Insert

   PLEKHA6 (PLEKHA6 Human)
   

   Use

   Tags

   GFP and Myc

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. PGK-HA-PGRN

   Plasmid

   #234872

   Purpose

   Lentiviral plasmid expressing HA-tagged human progranulin.

   Depositor

   Andrew Arrant

   Article

   Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi

   Insert

   Progranulin (GRN Human)
   

   Use

   Lentiviral

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDest17_N-myc

   Plasmid

   #234045

   Purpose

   Bacterial expression of N-terminally 6His-tagged N-myc; includes TEV cleavage site

   Depositor

   Tanja Mittag

   Article

   Yang et al Nat Struct Mol Biol. 2024 Oct;31(10):156

   Insert

   N-myc (MYCN Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. JDW 1392 (pLenti-CMV-FOXF1-P2A-H2A-mCherry)

   Plasmid

   #233546

   Purpose

   A CMV driven human FOXF1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   FOXF1 (FOXF1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. JDW 1391 (pME-V5-FOXF1-P2A-H2A-mCherry)

   Plasmid

   #232132

   Purpose

   A middle entry gateway clone containing V5 tagged human FOXF1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   FOXF1 (FOXF1 Human)
   

   Use

   Gateway cloning

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQE-meGFP-DLC1

    Plasmid

    #192048

    Purpose

    Plasmid expresses DLC1 binding peptide to Talin R8 domain in chimera with meGFP

    Depositor

    Ionel Popa

    Article

    Dahal et al Sci Adv. 2022 Jul 15;8(28):eabl7719. doi

    Insert

    DLC1 (DLC1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 945)

    Plasmid

    #229837

    Purpose

    A Piggybac compatible, tet-on, NEO selectable, vector for expressing luciferase followed by a P2A cleavage peptide and H2A fused mCherry.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 1154)

    Plasmid

    #229840

    Purpose

    A Piggybac compatible, tet-on, dual reporter encoding luciferase followed by a P2A cleavage peptide and then an H2A fused mCherry for nuclear labeling.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Str-KDEL_SBP-EGFP-PLAP

    Plasmid

    #222308

    Purpose

    Synchronize the trafficking of PLAP from the ER.

    Depositor

    Franck Perez

    Article

    Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

    Insert

    Streptavidin-KDEL and PLAP fused to SBP-EGFP (ALPP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    signal peptide removed

    Promoter

    CMV

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-Fgß-FIVAR-ELP-HIS-ybbR

    Plasmid

    #169136

    Purpose

    E. coli expression of FIVAR domain containing Fgß tag, ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    FIVAR (found in various architectures)
    

    Use

    Tags

    3xELP linker, 6x Histag, Fgβ peptide, and ybbr ta…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PTGFR-DuET

    Plasmid

    #213371

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    PTGFR (PTGFR Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MRGPRX4-DuET

    Plasmid

    #213347

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MRGPRX4 (MRGPRX4 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MRGPRX2-DuET

    Plasmid

    #213345

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MRGPRX2 (MRGPRX2 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MLNR-DuET

    Plasmid

    #213339

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MLNR (MLNR Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NMUR2-DuET

    Plasmid

    #213350

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    NMUR2 (NMUR2 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SSTR4-DuET

    Plasmid

    #213379

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    SSTR4 (SSTR4 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge