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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 12,887 results for: sequence

Showing: 4201 - 4220 of 12887 results

  1. pOpen-ETSSB

    Plasmid
    #165549
    Purpose
    ssDNA binding protein, 9kDa. Features: Improves the processivity of DNA polymerase; involved in stabilization and marking of ssDNA structure; increases the yield and specificitiy of PCR; increases the yield and processivity of RT during RT-PCR; improves DNA sequencing through regions with strong secondary structure
    Depositor
    FreeGenes Project, Jennifer Molloy, Drew Endy
    Insert
    Extreme Thermostable Single-Stranded DNA Binding Protein
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165549-plasmid-map-sequence-id-339430

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  4. pLEW-pHluorin2-PTS1

    Plasmid
    #213776
    Purpose
    Expresses the fluorescent protein pH sensor pHlorin2 in the glycosomes of Trypanosomal brucei
    Depositor
    Kenneth Christensen
    Insert
    pHlorin2
    Use
    Trypanosoma brucei expression
    Tags
    Expression
    Mutation
    Appended a 2 glycine linker and a T. brucei perox…
    Promoter
    T. brucei rRNA promotor
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    213776-plasmid-map-sequence-id-423197

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  5. pGFP FAK Y397F

    Plasmid
    #50516
    Purpose
    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Kenneth Yamada
    Insert
    protein tyrosine kinase, mutated
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Y397F
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. pAd/WT1-IRES-nAmCyan

    Plasmid
    #29756
    Depositor
    Edward McCabe
    Insert
    Wilms tumor 1 (WT1 Human)
    Use
    Tags
    AmCyan, IRES, and Nuclear localization sequence
    Expression
    Mammalian
    Mutation
    No mutations; AmCyan is nuclear localized; WT1 an…
    Promoter
    Available since
    Oct. 5, 2011
    Availability
    Academic Institutions and Nonprofits only
    29756-plasmid-map-sequence-id-137669

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  7. pSMH1741

    Plasmid
    #160930
    Purpose
    Expression of soluble Sc octamer (H3)
    Depositor
    Stephen Harrison
    Inserts
    HHF1
    HTA1
    HTB1
    HHT1
    Use
    Tags
    6His-TEV, N-6His-TEV, N-terminal 6His-TEV, and no…
    Expression
    Mutation
    codon-optimized for E. coli -- see provided seque…
    Promoter
    Available since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160930-plasmid-map-sequence-id-318962

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  8. pSMH1742

    Plasmid
    #160929
    Purpose
    Expression of soluble Sc octamer (Cse4)
    Depositor
    Stephen Harrison
    Inserts
    HHF1
    HTA1
    HTB1
    Cse4
    Use
    Tags
    6His-TEV, N-terminal 6His-TEV, and none
    Expression
    Mutation
    codon-optimized for E. coli -- see provided seque…
    Promoter
    Available since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160929-plasmid-map-sequence-id-319094

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  9. Q-ACACAA-10

    Plasmid
    #166546
    Purpose
    scFv of a human scaffold targeting Acetyl-CoA carboxylase 1. Antigen coverage aa 1572 - 2338 of 2346
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166546-plasmid-map-sequence-id-329238

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  10. Z-UBQLN2-7

    Plasmid
    #166563
    Purpose
    scFv of a human scaffold targeting Ubiquilin-2. Antigen coverage aa 1-624 of 624 aa, full-length
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    166563-plasmid-map-sequence-id-327818

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  11. A-BRDA4-3

    Plasmid
    #166513
    Purpose
    scFv of a human scaffold targeting Bromodomain-containing protein 4 isoform long. Antigen coverage aa 333-460 of 1362, Bromodomain 2
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    166513-plasmid-map-sequence-id-328227

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  12. Z-VCP-10

    Plasmid
    #166559
    Purpose
    scFv of a human scaffold targeting Transitional endoplasmic reticulum ATPase. Antigen coverage aa 1-806 of 806, full-length
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166559-plasmid-map-sequence-id-329267

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  13. p202 Dync1li1 wt

    Plasmid
    #26450
    Depositor
    Elizabeth Fisher
    Insert
    cytoplasmic dynein light intermediate chain 1 (Dync1li1 Mouse)
    Use
    Subcloning and sequencing
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Nov. 8, 2010
    Availability
    Academic Institutions and Nonprofits only

  14. pFHMSP-LIC-C

    Plasmid
    #26100
    Purpose
    Donor vector to generate recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector For use with Bac-to-Bac Expression System insect cells for secreted protein expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    8x His tag and MKFLVNVALVFMVVYISYIYAAA (Honeybee …
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. pT7-V5-SBP-C1-HsUpf1_1-925-siRNAres_P

    Plasmid
    #147191
    Purpose
    Mammalian Expression of HsUpf1_1-925-siRNAres
    Depositor
    Elisa Izaurralde
    Insert
    HsUpf1_1-925-siRNAres (UPF1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    4 non silent mutations A69S, E167G, K282R and A4…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147191-plasmid-map-sequence-id-430435

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  16. pT7-V5-SBP-C1-HsUpf1-del296-926_P

    Plasmid
    #147195
    Purpose
    Mammalian Expression of HsUpf1-del296-926
    Depositor
    Elisa Izaurralde
    Insert
    HsUpf1-del296-926 (UPF1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    4 non silent mutations A69S, E167G, K282R and T1…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147195-plasmid-map-sequence-id-430443

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  17. pT7-V5-SBP-C1-HsUpf1_115-1129-siRNAres_P

    Plasmid
    #147193
    Purpose
    Mammalian Expression of HsUpf1_115-1129-siRNAres
    Depositor
    Elisa Izaurralde
    Insert
    HsUpf1_115-1129-siRNAres (UPF1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    5 non silent mutations A69S, E167G, K282R, A442G…
    Promoter
    Available since
    March 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    147193-plasmid-map-sequence-id-355704

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  19. T-TRIM33-4

    Plasmid
    #166550
    Purpose
    scFv of a human scaffold targeting E3 ubiquitin-protein ligase TRIM33. Antigen coverage aa 882-1127 of 1127
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166550-plasmid-map-sequence-id-329241

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  20. Z-FARSB-5

    Plasmid
    #166556
    Purpose
    scFv of a human scaffold targeting phenylalanyl-tRNA synthetase, beta subunit. Antigen coverage aa 1-589 of 589, full-length
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166556-plasmid-map-sequence-id-329264

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Showing: 4201 - 4220 of 12887 results