We narrowed to 5,172 results for: PEP

1. pv7_CBX1_2xChromo_noEGFP

   Plasmid

   #179395

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo_noEGFP

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX1_2xChromo_noEGFP
   

   Use

   Tags

   AviTag

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Aug. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCEP4-MR1 (M50P)

   Plasmid

   #178651

   Purpose

   Mammalian expression of myc-tagged MR1 (M50P)

   Depositor

   Erik Procko

   Article

   McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

   Insert

   MR1 (MR1 Human)
   

   Use

   Tags

   Extracellular c-myc epitope ta and Signal peptide…

   Expression

   Mammalian

   Mutation

   M50P

   Promoter

   CMV

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEP4 MR1 (H83P)

   Plasmid

   #178652

   Purpose

   Mammalian expression of myc-tagged MR1 (H83P)

   Depositor

   Erik Procko

   Article

   McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

   Insert

   MR1 (MR1 Human)
   

   Use

   Tags

   Extracellular c-myc epitope ta and Signal peptide…

   Expression

   Mammalian

   Mutation

   H83P

   Promoter

   CMV

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP4 MR1 (Q153P)

   Plasmid

   #178653

   Purpose

   Mammalian expression of myc-tagged MR1 (Q153P)

   Depositor

   Erik Procko

   Article

   McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

   Insert

   MR1 (MR1 Human)
   

   Use

   Tags

   Extracellular c-myc epitope ta and Signal peptide…

   Expression

   Mammalian

   Mutation

   Q153P

   Promoter

   CMV

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pExpreS2-1-CR-PA

   Plasmid

   #175445

   Purpose

   HiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A tagged proteins with ExpreS2 Platform

   Depositor

   Wyatt Yue

   Article

   Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

   Type

   Empty backbone

   Use

   Tags

   BiP Secretion Peptide and Thrombin-Protein A

   Expression

   Insect

   Mutation

   Promoter

   Fused Actin-HSP70

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJ414-PCY1:S495A

   Plasmid

   #98151

   Purpose

   Expresses PCY1:S495A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1:S495A
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Changed serine 495 to alanine

   Promoter

   T7

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJ414-PCY1:W603A

   Plasmid

   #98153

   Purpose

   Expresses PCY1:W603A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1:W603A
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Changed tryptophan 603 to alanine

   Promoter

   T7

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJ414-PCY1:H695Q

   Plasmid

   #98154

   Purpose

   Expresses PCY1:H695Q from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1:H695Q
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Changed histidine 695 to glutamine

   Promoter

   T7

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJ414-PCY1:H695A

   Plasmid

   #98155

   Purpose

   Expresses PCY1H695A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1:H695A
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Changed histidine 695 to alanine

   Promoter

   T7

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJ414-PCY1:Y696G

    Plasmid

    #98156

    Purpose

    Expresses PCY1:R696G from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1:Y696G
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Changed tyrosine 696 to glycine

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV BARK R106A p2A mCherry

    Plasmid

    #117688

    Purpose

    CMV-iBARK(R106A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencing

    Depositor

    Baljit Khakh

    Article

    Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

    Insert

    BARKrgs R106A peptide
    

    Use

    Tags

    HA / FLAG

    Expression

    Mammalian

    Mutation

    R106A

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-GFP-EBA175-Flag(P1424G)

    Plasmid

    #155128

    Purpose

    Expresses N-terminally GFP-tagged EBA175 P1424G variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    P1424G; insert codes for EBA175 residues 1284 to …

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. D9_Ld_190920

    Plasmid

    #135825

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    peptidyl-prolyl cis-trans isomerase, macrophage infectivity potentiator precursor, putative
    

    Use

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCEP4-myc-H2-Dd-VN

    Plasmid

    #135517

    Purpose

    Mammalian expression of VN-fused and myc-tagged H2-Dd

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    H2-Dd (H2-D1 Mouse)
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    C121S

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCEP4-myc-H2-Ld-VN

    Plasmid

    #135520

    Purpose

    Mammalian expression of VN-fused and myc-tagged H2-Ld

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    H2-Ld
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCEP4-191084-QES.i06.c07

    Plasmid

    #123239

    Purpose

    Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 isolate; QES mutant with enhanced binding to PG16

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (191084 B7-19) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; Mutations P124D, …

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCEP4-001428-QES.c10.L544Y

    Plasmid

    #123254

    Purpose

    Mammalian expression plasmid for Env from the 001428 HIV-1 isolate; QES mutant with enhanced binding to PG16

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (001428) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; Mutations V208M, …

    Promoter

    CMV

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCEP4-Q842gp160-754*

    Plasmid

    #123231

    Purpose

    Mammalian expression plasmid for Env from the Q842.d12 HIV-1 isolate; C-terminal truncation

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (Q842.d12) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; Premature stop co…

    Promoter

    CMV

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCEP4-25711-QES.c11

    Plasmid

    #123219

    Purpose

    Mammalian expression plasmid for Env from the 25711 HIV-1 isolate; QES mutant with enhanced binding to PG16

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (25711) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; Mutations I181L, …

    Promoter

    CMV

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCEP4-ZM197M-QES.c10

    Plasmid

    #123224

    Purpose

    Mammalian expression plasmid for Env from the ZM197M HIV-1 isolate; QES mutant with enhanced binding to PG16

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (ZM197M) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; Mutations V208M, …

    Promoter

    CMV

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge