Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 13,768 results for: try

Showing: 4221 - 4240 of 13768 results

  2. Epac-S-H159

    Plasmid
    #170347
    Purpose
    mT2Del_EPACdDEPCD_tdBlackcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    mT2Del_EPACdDEPCD_tdBlackcp173Ven(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available Since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    170347-plasmid-map-sequence-id-336136

    Enlarge

  4. pDONR223 SARS-CoV-2 ORF10wu

    Plasmid
    #149332
    Purpose
    Gateway-compatible Entry vector, with insert of ORF10wu gene's CDS from SARS-CoV-2 genome definition in Wu et al, Nature 2020
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    ORF10wu (ORF10 SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Mutation
    Many synonymous changes due to codon optimization
    Available Since
    May 27, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149332-plasmid-map-sequence-id-298314

    Enlarge

  5. pDONR207 SARS-CoV-2 ORF7A_nostop

    Plasmid
    #149324
    Purpose
    Gateway-compatible Entry vector, with insert of ORF7A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    ORF7A (ORF7a SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Mutation
    Many synonymous changes due to codon optimization
    Available Since
    May 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149324-plasmid-map-sequence-id-296473

    Enlarge

  14. pOTTC292 - pAAV EF1a floxed hChR2(H134R)-EYFP

    Plasmid
    #50834
    Purpose
    An AAV packaging vector that expresses channel rhodopsin 2 (H134R) (fused to EYFP) under the EF1a promoter, which can be deactivated (deleted) by recombination between the flanking loxP sites.
    Depositor
    Brandon Harvey
    Article
    McDevitt et al Cell Rep. 2014 Sep 25;8(6):1857-1869. do
    Insert
    hChR2 (H134R)
    Use
    AAV and Cre/Lox
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    EF1a
    Available Since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    50834-plasmid-map-sequence-id-76270

    Enlarge

  17. pKK-RNAi-nucEGFPmiR-TEV-FLAG

    Plasmid
    #105811
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal FLAG.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    TEV-FLAG
    Expression
    Mammalian
    Available Since
    March 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    105811-plasmid-map-sequence-id-204856

    Enlarge

Showing: 4221 - 4240 of 13768 results