We narrowed to 9,661 results for: try

1. pLCKO-CMV-TMEM106B-P2A-Blasticidin

   Plasmid

   #237478

   Purpose

   Encodes full length human TMEM106B protein, along with blasticidin selection marker.

   Depositor

   Dirk Daelemans

   Article

   Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

   Insert

   TMEM106B (TMEM106B Human)
   

   Use

   Lentiviral

   Tags

   P2A-Blasticidin

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Mouse

   Plasmid

   #238133

   Purpose

   This plasmid encodes the full-length Mouse (Mus musculus) TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.

   Depositor

   Dirk Daelemans

   Article

   Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

   Insert

   TMEM106B (Tmem106b Mouse)
   

   Use

   Lentiviral

   Tags

   Blasticidin-P2A-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-SpyTag-Flag-dNS-SRRM1-V5-mCherry

   Plasmid

   #235096

   Purpose

   SRRM1 lacking Nuclear Speckle domain (dNS-SRRM1) mCherry fusion protein without MCP domain

   Depositor

   Mitchell Guttman

   Article

   Bhat et al Nature. 2024 May;629(8014):1165-1173. do

   Insert

   pCAG-SpyTag-Flag-dNS-SRRM1-V5-mCherry (SRRM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The SRRM1 entry clone from DNASU was modified usi…

   Promoter

   CAG

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-RnSyt1-sgRNA2-dCas9-KRAB-TagBFP2 (identifier AAAA-0246)

   Plasmid

   #202555

   Purpose

   Synaptotagmin-1 sgRNA2 with TagBFP2

   Depositor

   Frederic Meunier

   Article

   Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

   Insert

   CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCAGTACTCGCGTGCCTCGCACCGG) (Syt1 Rat)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

   Plasmid

   #202556

   Purpose

   Synaptotagmin-1 sgRNA3 with TagBFP2

   Depositor

   Frederic Meunier

   Article

   Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

   Insert

   CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-RnSyt1-sgRNA1-dCas9-KRAB-TagBFP2 (identifier AAAA-0245)

   Plasmid

   #202554

   Purpose

   Synaptotagmin-1 sgRNA1 with TagBFP2

   Depositor

   Frederic Meunier

   Article

   Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

   Insert

   CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ -CACCGCGTGCCTCGCACCGGTCCGCGG) (Syt1 R. norvegius (gRNA))
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCR8/GW_TOPO_Cdk13_Nterm_FlagHa

   Plasmid

   #127343

   Purpose

   N-terminal Flag- HA- tandem epitope tagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into Gateway entry vector

   Depositor

   Phil Sharp

   Article

   Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

   Insert

   Cyclin Dependent Kinase 13 (Cdk13 Mouse)
   

   Use

   Entry vector with transgene for gateway cloning

   Tags

   Flag- HA- Tandem Epitopes

   Expression

   Mutation

   Base pairs 1-1554 of transgene are codon optimize…

   Promoter

   None

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTXBI-mclover-YTH-IDR2

   Plasmid

   #177122

   Purpose

   Bacterial expression of YTH-IDR2 fragment of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification

   Depositor

   Wenbo Li

   Article

   Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

   Insert

   YTHDC1 (YTH and IDR2 domains) (YTHDC1 Human)
   

   Use

   Tags

   Mxe GyrA intein-Chitin binding domain for protein…

   Expression

   Bacterial

   Mutation

   YTH and IDR2 domains only (IDR1 deletion)

   Promoter

   T7

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFOSGE_COF2F04

   Plasmid

   #70901

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   mod(mdg4) (mod(mdg4) Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pLV[TetOn]-Puro-TRE3G>mNeurog2

    Plasmid

    #198754

    Purpose

    Vector encodes the gene for murine neurogenin-2 under control of a Tet-controlled promoter and the puromycin resistance gene under control of a constitutive PGK promoter

    Depositor

    Nael Nadif Kasri

    Article

    Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

    Insert

    Neurogenin-2 (Neurog2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV[TetOn]-Puro-TRE3G>mAscl1

    Plasmid

    #198755

    Purpose

    Vector encodes the gene for murine Ascl1 under control of a Tet-controlled promoter and the puromycin resistance gene under control of a constitutive PGK promoter

    Depositor

    Nael Nadif Kasri

    Article

    Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

    Insert

    Ascl1 (Ascl1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid

    #223065

    Purpose

    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    

    Use

    CRISPR

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Bacterial

    Mutation

    Three regions of SpCas9 encode type IIS restricti…

    Promoter

    Dual T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL4-eIF3B

    Plasmid

    #133308

    Purpose

    Expression of luciferase driven by eIF3B promoter region

    Depositor

    Josep Domingo-Domenech

    Article

    Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

    Insert

    eIF3B promoter (EIF3B Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    eIF3B

    Available since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLCKO-CMV-SARS-CoV-2-S-Bel-P5_5-7-IRES-mNeonGreen-NES/PKI-P2A-Blasticidin

    Plasmid

    #237480

    Purpose

    Encodes full-length SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate), featuring the E484D substitution, which likely emerged during passaging and enhances TMEM106B binding.

    Depositor

    Dirk Daelemans

    Article

    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

    Insert

    SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate)
    

    Use

    Lentiviral

    Tags

    IRES-mNeonGreen-NES/PKI and P2A-Blasticidin

    Expression

    Mammalian

    Mutation

    S484D + S813I + deleted amino acids 68-76 + 676-6…

    Promoter

    CMV

    Available since

    July 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLCKO-CMV-TMEM106B-M210A_F213A-P2A-Blasticidin

    Plasmid

    #237479

    Purpose

    Encodes full-length human TMEM106B. M210A/F213A mutations block S1 binding and SARS-CoV-2 Belgium/GHB-03021/2020 TMEM106B mediated infection, without affecting TMEM106B expression or localization.

    Depositor

    Dirk Daelemans

    Article

    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

    Insert

    TMEM106B (TMEM106B Human)
    

    Use

    Lentiviral

    Tags

    P2A-Blasticidin

    Expression

    Mammalian

    Mutation

    M210A + F213A

    Promoter

    CMV

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL4-eIF3B MBE3 mutant

    Plasmid

    #133310

    Purpose

    Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 3

    Depositor

    Josep Domingo-Domenech

    Article

    Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

    Insert

    eIF3B promoter MBE3 mutant (EIF3B Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    ccacgtgacc changed to cAaAAAAacc

    Promoter

    eIF3B

    Available since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL4-eIF3B MBE2 mutant

    Plasmid

    #133309

    Purpose

    Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 2

    Depositor

    Josep Domingo-Domenech

    Article

    Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

    Insert

    eIF3B promoter MBE2 mutant (EIF3B Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    gccacatgcacc changed to gcAaAaAAcacc

    Promoter

    eIF3B

    Available since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)

    Plasmid

    #202553

    Purpose

    Nontargeting control vector with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    Humanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Expression

    Mutation

    Puromycin-resistance gene in the pLV hU6-sgRNA hU…

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MultiSite Gateway plasmid toolKit

    Plasmid Kit

    #1000000050

    Purpose

    Cloning kit for constructing fully-customizable three-part fusion proteins, based on MultiSite Gateway from Life Technologies. 12 promoter-less Gateway entry clones, N- and C-term flanking modules

    Depositor

    Tomas Santalucia

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

20. RAS mutant clone collection

    Plasmid Kit

    #1000000089

    Purpose

    Wild-type and mutant HRAS, NRAS, KRAS4a and KRAS4b gene plasmids as Gateway entry clones

    Depositor

    Dominic Esposito

    Available since

    Dec. 1, 2016

    Availability

    Academic Institutions and Nonprofits only