Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

199,412 results

Showing: 2121 - 2130 of 199412 results

  2. Cultivarium POSSUM Toolkit

    Plasmid Kit
    #1000000234
    Purpose
    Golden Gate genetic toolkit developed to help researchers identify functional plasmids and establish genetic tractability in non-model bacteria.
    Depositor
    Nili Ostrov
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate (Loop)
    Available Since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only

  4. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid
    #139987
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139987-plasmid-map-sequence-id-275335

    Enlarge

  5. AAV-GFP/Cre

    Plasmid
    #49056
    Purpose
    Adeno-associated virus (AAV) encoding a green fluorescent protein/Cre recombinase (GFP/Cre) fusion protein.
    Depositor
    Fred Gage
    Article
    Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99
    Insert
    Cre
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    EGFP, Myc, and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    49056-plasmid-map-sequence-id-182646

    Enlarge

  6. pLenti_P2A_Blast

    Plasmid
    #211365
    Purpose
    Lentiviral expression vector for an insert of interest linked to a P2A-T2A blasticidin sequence. Produces virus very efficiently. Can also be used for regular transfections
    Depositor
    Jacques Neefjes
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    211365-plasmid-map-sequence-id-437557

    Enlarge

  8. pSFV-Helper1

    Plasmid
    #92073
    Purpose
    This is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.
    Depositor
    Henrik Garoff
    Article
    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356
    Insert
    cDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
    Use
    Helper plasmid, for run-off transcription and rna…
    Available Since
    July 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92073-plasmid-map-sequence-id-275942

    Enlarge

Showing: 2121 - 2130 of 199412 results