We narrowed to 10,368 results for: Coli
-
Plasmid#227224PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
Tags6HisExpressionBacterialMutationE345KPromoterT7-promoterAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
V485G FL USP7
Plasmid#227222PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
Tags6HisExpressionBacterialMutationV485GPromoterT7-promoterAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-a(+)-6xHis-mScarlet-I(+5)
Plasmid#219418PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorInsertmScarlet-I(+5)
TagsMGHHHHHGGExpressionBacterialMutationD125K, D186K, E216K, E238KPromoterT7Available SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LflChmG
Plasmid#221343PurposeExpress LflChmG in E. coliDepositorInsertchmG
TagsHis6ExpressionBacterialAvailable SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LflChmH
Plasmid#221344PurposeExpress LflChmH in E. coliDepositorInsertchmH
TagsHis6ExpressionBacterialAvailable SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ChmF
Plasmid#221345PurposeExpress ChmF in E. coliDepositorInsertchmF
TagsHis6ExpressionBacterialAvailable SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ChmK
Plasmid#221346PurposeExpress ChmK in E. coliDepositorInsertchmK
TagsHis6ExpressionBacterialAvailable SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ChmL
Plasmid#221347PurposeExpress ChmL in E. coliDepositorInsertchmL
TagsHis6ExpressionBacterialAvailable SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ChmJ
Plasmid#221342PurposeExpress ChmJ in E. coliDepositorInsertchmJ
TagsHis6ExpressionBacterialAvailable SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-H12TEV-hEIF5B-587~1220
Plasmid#208637Purposefor E. coli expression truncated human eIF5B (ENST00000289371), aa587~1220DepositorInserthuman eIF5B (ENST00000289371) aa 587~1220 (EIF5B Human)
Tags6xhisExpressionBacterialMutationcontain SNP rs1376443, protein sequence not chang…PromoterT7Available SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-mCherry(-6)-L1
Plasmid#205046PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertmCherry(-6)-L1
TagsMGHHHHHGGExpressionBacterialAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-mCherry(-6)-L2
Plasmid#205047PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertmCherry(-6)-L2
TagsMGHHHHHGGExpressionBacterialAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-mCherry(-6)-L3
Plasmid#205048PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertmCherry(-6)-L3
TagsMGHHHHHGGExpressionBacterialAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-L1
Plasmid#205027PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-L1
TagsKGRRGKKSRK and MGHHHHHGGAExpressionBacterialAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-L2
Plasmid#205028PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-L2
TagsKGRRGKKSRKGRRGKKSRK and MGHHHHHGGAExpressionBacterialAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-L3
Plasmid#205029PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-L3
TagsKGRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGAExpressionBacterialAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-H1
Plasmid#205030PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H1
TagsKKRRRKK and MGHHHHHGGAExpressionBacterialAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-H2
Plasmid#205031PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H2
TagsKKRRRKKKRRRKK and MGHHHHHGGAExpressionBacterialAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-H3
Plasmid#205032PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H3
TagsKKRRRKKKRRRKKKRRRKK and MGHHHHHGGAExpressionBacterialAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-Δ1~34,Δ215~284-TwStrep
Plasmid#208630Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa35~398 with deletion of 215~284, fused with Twin Strep tag.DepositorInserthuman RPL3 (ENST00000216146) Δ1~34, Δ215~284 (RPL3 Human)
Tags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationΔ1~34,Δ215~284PromoterT7Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTatABmNeonC
Plasmid#178464PurposepTatABC with an mNeonGreen fluorescent protein tag at the C-terminal end of TatB.DepositorInsertTatA, TatBmNeon, TatC
TagsmNeonGreen fused to TatBExpressionBacterialPromoteraraCAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pE5772
Plasmid#170779Purposeexpression of hybrid proteins in E. coliDepositorInsertGentR
TagspolyhisbioMbpTEVExpressionBacterialPromoterT7Available SinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSecEffector wo TEVp
Plasmid#172892Purposeatc inducible (in any E. coli strain) BxbI and arabinose inducible TP901DepositorInsertsBxb1
TP901
ExpressionBacterialPromoternTETO and pBAD /AraCAvailable SinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDest-894 (attR4-attR2)
Plasmid#161893PurposeMultisite Gateway (attR4-attR2) vector for expression of multiple proteins in E. coliDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPMY1CB0001
Plasmid#162317PurposeAcceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven expression in E. coli. Contains mRFP1 reporter.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0KN0025
Plasmid#162283PurposeAcceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for T7-driven E. coli cell-free protein synthesisDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUI-SynP-CAS1sg
Plasmid#154341PurposeExpression of the sgRNA targeting to the E. coli can geneDepositorInsertCAS1 sgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUI-SynP-CAS2sg
Plasmid#154342PurposeExpression of the sgRNA targeting to the E. coli can geneDepositorInsertCAS2 sgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUI-SynP-CANTsg
Plasmid#154343PurposeExpression of the sgRNA targeting to the E. coli can geneDepositorInsertCANT sgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUI-SynP-CAPsg
Plasmid#154344PurposeExpression of the sgRNA targeting to the E. coli can geneDepositorInsertCAP sgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only