We narrowed to 34,047 results for: CMV

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pSIN-TRE-H2BeGFP-rtTA2

Plasmid

#165494

Purpose

Doxycycline-inducible all-in-one lentivirus vector to express H2BeGFP fusion protein. System for labelling slow-cycling cells in vivo.

Depositor

Hector G. Palmer

Article

Puig et al J Clin Invest. 2018 Aug 31;128(9):3887-3

Insert

Human histone H2B (H2BC21 Human)

Use

Lentiviral

Tags

EGFP

Mutation

-

Promoter

TetO-CMV

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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Nsp3 -mCherry

Plasmid

#165131

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp3 (ORF1ab codon optimized SARS-COV-2)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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ABE8.8-m

Plasmid

#136294

Purpose

expresses ABE8.8-m in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.8-m

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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HA-GFP

Plasmid

#137763

Purpose

Control plasmid for immunoprecipitation of HA-tagged proteins. Also a good control for transfection efficiency.

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GFP

Tags

HA and myc

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-WT-VWF

Plasmid

#124794

Purpose

Expresses multimeric human VWF with C-terminal c-Myc tag

Depositor

Sriram Neelamegham

Article

Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

Insert

VWF (VWF Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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HA-14-3-3 epsilon

Plasmid

#116886

Purpose

Expresses HA-tagged human 14-3-3 epsilon in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Article

Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

Insert

14-3-3 epsilon (YWHAE Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pTO_CHMP4B_LAP

Plasmid

#101850

Purpose

Tet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration

Depositor

Daniel Gerlich

Article

Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d

Insert

Charged Multivesicular Body Protein 4B (CHMP4B Human)

Use

Gateway cloning, flp-frt recombination, tet-induc…

Tags

Localization and Affinity Purification (LAP) tag

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-teLuc c-myc

Plasmid

#100026

Purpose

Enhanced red-shifted bioluminescence when paired with diphenylterazine (DTZ)

Depositor

Huiwang Ai

Article

Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

Insert

teLuc, a NanoLuc mutant

Tags

c-myc

Expression

Mammalian

Mutation

D19S, D85N, C164H

Promoter

CMV

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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HA-Rab11-WT

Plasmid

#101047

Purpose

Expresses HA-tagged human Rab11-WT in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

RAB11 (RAB11A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-FRB-Cbir_Myc-NBir-FKBP

Plasmid

#90008

Purpose

Split-BioID plasmid: Expresses C-terminally tagged FRB-CBir[257-321] and N-terminally tagged NBir[2-256]-FKBP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

NBirA*-linker-FKBP
FRB-linker-CBirA*

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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mApple-Sec61b-C1

Plasmid

#90993

Purpose

Expresses mApple-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

Depositor

Jennifer Lippincott-Schwartz

Article

Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

Insert

mApple-Sec61b (SEC61B Synthetic, Human)

Tags

mApple (red fluorescence)

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-MTS-TagBFP-P2AT2A-EGFP-NLS-P2AT2A-mCherry-PTS1

Plasmid

#87829

Purpose

High-efficient mammalian expression vector for co-expression of BFP-, EGFP- and mCherry-tagged proteins using P2AT2A. MTS, NLS and PTS1 can be replaced by proteins of interest.

Depositor

Andrea Musacchio

Article

Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

Inserts

MTS
NLS
PTS1

Expression

Mammalian

Promoter

CMV promoter, tetracycline operator

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pSkylan-NS-N1

Plasmid

#86785

Purpose

for fusion of gene of interest with a monomeric reversibly photoswitchable fluorescent protein, Skylan-NS

Depositor

Pingyong Xu

Article

Zhang et al Proc Natl Acad Sci U S A. 2016 Sep 13;11

Type

Empty backbone

Tags

Skylan-NS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-NSF

Plasmid

#74924

Purpose

Mammalian expression of human NSF full-length (744 aa)

Depositor

Elisa Greggio

Article

Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

Insert

NSF (NSF Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pclbw-mitoTagRFP

Plasmid

#58425

Purpose

Mammalian expression of tagRFP targeted to the mitochondrial matrix

Depositor

David Chan

Article

Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

Insert

mito-tagRFP

Use

Retroviral

Expression

Mammalian

Promoter

cmv-bactin

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-mouse vinculin full length (889)

Plasmid

#67935

Purpose

Mammalian expression of mouse vinculin with GFP fusion

Depositor

Alpha Yap

Article

Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

Insert

Vinculin (Vcl Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

L159P in vinculin

Promoter

CMV

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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GIV-WT - FLAG

Plasmid

#65947

Purpose

Expression GIV/Girdin in Mammalian cell

Depositor

Pradipta Ghosh

Article

Lin et al Mol Biol Cell. 2014 Nov 5;25(22):3654-71

Insert

GIV/Girdin (CCDC88A Human)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only
pCDH_Str-KDEL_neomycin

Plasmid

#65307

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end

Use

Lentiviral

Tags

KDEL motif and Signal Sequence

Promoter

CMV

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cyto-CaNAR2

Plasmid

#64729

Purpose

Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNAR2-NES

Tags

Cerulean3, Nuclear export signal (NES), and YPet

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-TAF1

Plasmid

#65395

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

TAF1 (TAF1 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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