We narrowed to 9,351 results for: pho;

1. pMXS-IRES-BLAST flag-CAD T456A S1859A

   Plasmid

   #169824

   Purpose

   Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and S6K phosphorylation site

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   T456A S1859A; TCCC -> AGTC silent mutations at…

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMCRW1

   Plasmid

   #122462

   Purpose

   Human NPM-ALK fusion protein gene in pET28a bacterial expression plasmid. Production of recombinant human NPM-ALK fusion protein in E. coli Bl21 Rosetta2 cells

   Depositor

   Richard C. Willson

   Article

   Kourentzi et al Sci Rep. 2020 Mar 19;10(1):5078. doi: 10

   Insert

   NPM-ALK fusion protein (ALK, NPM1 Human)
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac promoter

   Available since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223-PRPS1

   Plasmid

   #23588

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   PRPS1 (PRPS1 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pME-mCherry-PA-Rac1

   Plasmid

   #169042

   Purpose

   Middle entry vector for Gateway cloning that contains an mCherry fused with an optimized photoactivatable Rac1 gene insert

   Depositor

   Paola Arlotta

   Article

   Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

   Insert

   PA-Rac-1 (RAC1 Human, Avena sativa (oat))
   

   Use

   Gateway entry cloning vector

   Tags

   6X His and Fused with mCherry

   Expression

   Mutation

   Rac1 starts at I4 and contains mutations Q61L, E9…

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDestTol2CG2-mnx1:mCherry-PA-Rac1

   Plasmid

   #169044

   Purpose

   Destination transgenesis vector for expressing mCherry fused with an optimized photoactivatable Rac1 protein in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer.

   Depositor

   Paola Arlotta

   Article

   Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

   Insert

   PA-Rac-1 (RAC1 Human, Avena sativa (oat))
   

   Use

   Tags

   6X His and Fused with mCherry

   Expression

   Mammalian

   Mutation

   Rac1 starts at I4 and contains mutations Q61L, E9…

   Promoter

   3xMnx1

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PGKp-GFP-YAP (5SA)

   Plasmid

   #174174

   Purpose

   Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the human PGK promoter.

   Depositor

   Rod Bremner

   Article

   Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

   Insert

   YAP (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   3x FLAG tag

   Expression

   Mammalian

   Mutation

   Five LATS phosphorylation sites (S61, S109, S127,…

   Promoter

   PGK

   Available since

   Aug. 19, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. EFSp-GFP-YAP (5SA)

   Plasmid

   #174170

   Purpose

   Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the EFS promoter.

   Depositor

   Rod Bremner

   Article

   Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

   Insert

   YAP (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   3x FLAG tag

   Expression

   Mammalian

   Mutation

   Five LATS phosphorylation sites (S61, S109, S127,…

   Promoter

   EFS

   Available since

   Sept. 28, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. Human Kinase Domain Constructs for Automated Bacterial Expression

   Plasmid Kit

   #1000000094

   Purpose

   68 His-tagged human kinase catalytic domain constructs and the respective phosphatases that enhance their bacterial expression; optimized for automated bacterial expression

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

9. Anti-Fig4/Sac3 [N202/7R] (Recombinant Antibody)

   Antibody

   #192037-rAb

   Purpose

   Anti-Fig4/Sac3 (Mouse) recombinant mouse monoclonal antibody

   Depositor

   James Trimmer

   Article

   Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

   Recommended Applications

   Immunocytochemistry and Western Blot

   Reactivity

   Human, Mouse, and Rat

   Source Species

   Mouse

   Isotype

   IgG2a

   Trial Size

   Available to purchase

   Available since

   Jan. 30, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

10. pGLU_115

    Plasmid

    #225795

    Purpose

    RS421 library variant 14067

    Depositor

    Nili Ostrov

    Insert

    14067_[Tn5]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGLU_79

    Plasmid

    #225780

    Purpose

    RS421 library variant 14067

    Depositor

    Nili Ostrov

    Insert

    14067_[abr]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGLU_97

    Plasmid

    #225810

    Purpose

    RS421 library variant 14067

    Depositor

    Nili Ostrov

    Insert

    14067_[Himar]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. PA-GFP-α-Tubulin

    Plasmid

    #213900

    Purpose

    Imaging of Tubulin using Photoactivatable GFP

    Depositor

    Lukas Kapitein

    Insert

    PA-GFP-α-Tubulin
    

    Use

    Tags

    Photoactivatable GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR4-SB1-LUC (w832-1)

    Plasmid

    #29647

    Depositor

    Eric Campeau

    Insert

    Firefly Luciferase
    

    Use

    Entry vector

    Tags

    SB1

    Expression

    Mutation

    Promoter

    Available since

    May 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDH-PGK

    Plasmid

    #72268

    Purpose

    Express gene of interest under PGK (phosphoglycerate kinase I) promoter

    Depositor

    Kazuhiro Oka

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 11, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pET28b_NOSER

    Plasmid

    #124244

    Purpose

    Bacterial 6xHIS tagged protein expression without the addition of any potential phosphorylation sites to the tagged protein.

    Depositor

    Attila Fehér

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAGNT-Sema3A

    Plasmid

    #16428

    Depositor

    Jonathan Raper

    Insert

    Sema3A (SEMA3A Chicken)
    

    Use

    Tags

    His and Myc

    Expression

    Mutation

    Promoter

    Available since

    Dec. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pQCXIP-F-luc

    Plasmid

    #73058

    Purpose

    Retroviral expression of firefly luciferase for in vivo imaging

    Depositor

    Michael Grusch

    Insert

    Firefly luciferase
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. CMV-SEAP

    Plasmid

    #24595

    Depositor

    Alan Cochrane

    Insert

    SEAP (ALPP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCherry-myosinRLC

    Plasmid

    #105761

    Purpose

    Red NMMII phosphorylation sensor

    Depositor

    Megan Rizzo

    Insert

    mCherry-RLC
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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