We narrowed to 6,826 results for: poly

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psicheck-2-Casp8

Plasmid

#48172

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of CASP8 (CASP8 Human)

Use

Luciferase

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
pXPA-fapO-RgTALsyn

Plasmid

#100946

Purpose

Expression of Tyrosine Ammonia Lyase from constitutive GAP promoter

Depositor

Mattheos Koffas

Article

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Insert

RgTALsyn

Use

Synthetic Biology

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

GAP (Constitutive)

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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Frt-hspB10

Plasmid

#63101

Purpose

Expression of HSPB10 (small HSP) in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb10 (ODF1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-Ataxin3Q22-C14A

Plasmid

#22113

Depositor

Henry Paulson

Article

Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

Insert

Ataxin 3 (ATXN3 Human)

Tags

GST

Expression

Bacterial

Mutation

C14A

Available Since

Sept. 16, 2009

Availability

Academic Institutions and Nonprofits only
pPSP6+t-gfp

Plasmid

#48130

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-SP6-inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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PB-Haunt E1-PuroT2AmCherry-4A KI

Plasmid

#92164

Purpose

For lncRNA 4_pA(3_SV40 polyA and 1 BGH polyA) homologue recombination mediated knockin

Depositor

Xiaohua Shen

Article

Yin et al Cell Stem Cell. 2015 May 7;16(5):504-16.

Insert

Haunt P-Inr2-cDNA KI homology arms

Use

Other

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shStk16-1

Plasmid

#180391

Purpose

Producing AAV that encodes mouse Stk16 shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shStk16-1 (Stk16 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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DsRed +9e

Plasmid

#191766

Purpose

Expression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9e

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

DsRed

Tags

N terminal fusion of positively charged polypetod…

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pMH-HT_SsCHAD-H29A-R32A-R36A-R69A

Plasmid

#127787

Purpose

Expresses a SsCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

SsCHAD (SULM_RS11045 Sulfolobus solfataricus)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

mutations in His29, Arg32, Arg36 and Arg69 to Ala

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pIS1 DICER1 long-mut-let-7

Plasmid

#21651

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Dicer 1 (DICER1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant let-7 microRNA binding sites

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pCDF11-SFPQ(214-598)

Plasmid

#135440

Purpose

Expresses SFPQ 214-598 with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

splicing factor proline and glutamine rich (SFPQ Human)

Tags

Hexahistidine

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pIS1 DICER1 long-mut-all

Plasmid

#21653

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Dicer 1 (DICER1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutated let-7 and miR103/107 microRNA binding sit…

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pIS1 DICER1 long-mut-miR103/107

Plasmid

#21652

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Dicer 1 (DICER1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant miR103/107 microRNA binding sites

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pCDF11-SFPQ(276-535)

Plasmid

#135436

Purpose

Expresses SFPQ 276-535 with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

splicing factor proline and glutamine rich (SFPQ Human)

Tags

Hexahistidine

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLJM1-SLC35B2-FLAG

Plasmid

#154863

Purpose

Transient or Lentiviral expression of sgRNA (Addgene#154860) resistant SLC35B2

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

SLC35B2 (SLC35B2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

ctcctggtgcagtacttc => ttattagtccaatatttt

Promoter

CMV

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pIS1 IMP-1 long-mut-let-7

Plasmid

#21642

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

IGF-II mRNA-binding protein 1 (IGF2BP1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant let-7 microRNA binding sites

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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R4pGWB401+Citrine

Plasmid

#128432

Purpose

Modified R4pGWB401 three way Gateway destination vector (Nakagawa et al. 2008) that enables C-terminal fusion of Citrine, a dimeric acid stable yellow fluorescent protein.

Depositor

George Haughn

Article

McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100

Type

Empty backbone

Use

Gateway destination vector

Tags

Citrine, yellow fluorescence protein

Expression

Plant

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET30a-ASXL1-HARE-HTH domain

Plasmid

#107164

Purpose

Expresses murine ASXL1 HARE-HTH domain (amino acids 2-83) in E. coli

Depositor

Douglas Feldman

Article

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

ASXL1 (Asxl1 Mouse)

Tags

Flag

Expression

Bacterial

Mutation

Contains amino acids 2-83

Promoter

T7

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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Frt-V5-hspB10

Plasmid

#63111

Purpose

Expression of HSPB10 (small HSP) tagged with V5 in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb10 (ODF1 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-Ataxin3Q22-UIM*

Plasmid

#22114

Depositor

Henry Paulson

Article

Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

Insert

Ataxin 3 (ATXN3 Human)

Tags

GST

Expression

Bacterial

Mutation

UIM 1, 2, 3 SA-DG

Available Since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only