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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,582 results for: eng

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Showing: 4261 - 4280 of 14582 results

  4. Zhou-COVID-19-Spike

    Plasmid
    #161029
    Purpose
    Extracellular domain of SARS-CoV-2 spike protein that has been engineered so that it assembles pseudo virus more efficiently. Also contains portions of VSV-G for production of psuedovirus.
    Depositor
    Dapeng Zhou
    Publication
    Tong et al Vaccines (Basel). 2021 Mar 22;9(3). pii:
    Insert
    Engineered COVID-19 Spike protein, VSV-G (S SAR-CoV-2, Vesicular stomatitis virus)
    Expression
    Mammalian
    Mutation
    Codon optimized
    Available since
    Jan. 12, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161029-plasmid-map-sequence-id-320615

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  5. Endoplasmic reticulum-targeted optodPLD

    Plasmid
    #140059
    Purpose
    Encodes CRY2-mCherry-dPLD-P2A-CIBN-Sac1(TM) for mammalian expression
    Depositor
    Jeremy Baskin
    Publication
    Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Phospholipase D
    Tags
    CIBN, CRY2, P2A, Sac1(TM), and mCherry
    Expression
    Mammalian
    Mutation
    H170A (catalytic dead)
    Available since
    March 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    140059-plasmid-map-sequence-id-275746

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  10. TALEN-CCR5L18-CtermQ7

    Plasmid
    #51442
    Purpose
    Expresses TALEN targeting left CCR5A site (L18) with Q7 C-terminal domain
    Depositor
    David Liu
    Publication
    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:
    Insert
    TALEN CCR5 Left (L18) Q7 C-term
    Use
    TALEN
    Tags
    3xFLAG and Fok1 EL variant
    Expression
    Mammalian
    Mutation
    K777Q, K778Q, K788Q, R789Q, R792Q, R793Q and R801Q
    Promoter
    CMV
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51442-plasmid-map-sequence-id-78450

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  14. pcDNA3.1_ionRE

    Plasmid
    #172932
    Purpose
    Ionic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.
    Depositor
    Arnold Boersma
    Publication
    Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IonicStrengthSensorRE
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    172932-plasmid-map-sequence-id-339435

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  15. snuABE4.1

    Plasmid
    #246013
    Purpose
    snuABE4.1 composed of PhADAR(E438Q, I536S, E637F) with 10a.a truncation fused to SpCas9 nickase [R221K, N394K, H840A] via an SV40 linker and further fused to La protein.
    Depositor
    Sangsu Bae
    Publication
    Im et al bioRxiv 2025.07.19.664718
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Pediculus humanus ADAR deaminase domain (E438Q, I536S, E637F mutant)
    Expression
    Mammalian
    Mutation
    E438Q, I536S, E637F, with 10 amino acids truncati…
    Promoter
    CMV
    Available since
    Nov. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    246013-plasmid-map-sequence-id-492306

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  19. scFv-GCN4-DNMT3a-DNMT3l

    Plasmid
    #154140
    Purpose
    Expresses the scFv-GCN4-DNMT3a-DNMT3l fusion protein (more details are shown in the vectro map) for targeted DNA methylation. Should be used in a combination with the dCas9-SunTag systems.
    Depositor
    Albert Jeltsch
    Publication
    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
    Tags
    HA and sfGFP
    Expression
    Mammalian
    Promoter
    SFFV
    Available since
    Oct. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    154140-plasmid-map-sequence-id-345614

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Showing: 4261 - 4280 of 14582 results