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pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast
Plasmid#138149PurposeExpresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleusDepositorInsertCasRx
UseLentiviralTagsNLS-HAExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
VRQR variant
Plasmid#138562PurposeExpresses human codon-optimized SpCas9-VRQR and blasticidin resistance: EFS promoter-VRQR-NLS-FLAG-P2A-BSDDepositorInsertVRQR
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationD1135V, G1218R, R1335Q, T1337RPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NRTH
Plasmid#198541PurposeExpresses human codon-optimized SpCas9-NRTH and blasticidin resistance: EFS promoter-SpCas9-NRTH-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRTH
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Target-AIDmax
Plasmid#139107PurposeMammalian Target-AIDmax expression vector (pSI-626)DepositorInsertBi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
UseCRISPRTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSII-EF/mt-(n1)StayGold
Plasmid#185823PurposeA soluble photostable fluorescent marker of the mitochondrial matrix. The StayGold gene has codon optimization for mammalian expression.DepositorInsertmt-(n1)StayGold
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1363 LV EF1a-CD19 IRES-EGFP
Plasmid#201919Purpose2nd generation lentiviral transfer plasmid for engineering cells to express human CD19DepositorInsertCD19 (CD19 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGK-HELLO LV
Plasmid#202001PurposeExpresses HELLO neoantigen in tumor cellsDepositorInsertHELLO
UseLentiviralTagsFLAGExpressionMutationPromoterpGKAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.sgRNA.EFS.GFP
Plasmid#57822Purpose3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter drivenDepositorInsertssgRNA
EFS
eGFP
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-pGK-Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro
Plasmid#115684PurposeNanoLuc-Firefly Luciferase fusion with constitutive nuclear expression for calibration of Nanoluc signal to Firefly Luciferase signal. H2B-mCherry marks chromatin with red fluorescence.DepositorInsertNluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMV_AA050
Plasmid#216107PurposegDNA expression for Cas9 with ChenF+E trRNA (guide only)DepositorInsertgDNA expression for Cas9 with ChenF+E trRNA
UseCRISPR and Lentiviral; Assembled vectorTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL1777 (pEffector)
Plasmid#160731PurposeEffector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.DepositorInsertVchCAST proteins and crRNA
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
CRISPR-StAR4GN (Lenti)
Plasmid#222693PurposeCRISPR-screeningDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsGFPExpressionMammalianMutationPGK without BsmBI cutsitePromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL1765 (pSPIN-R, R*MmeI)
Plasmid#160734PurposeSingle-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
gCH27 (crCD55-4)
Plasmid#217343PurposecrRNA targeting CD55DepositorInsertcrCD55-4 (CD55 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
gRNA NIA TLS2
Plasmid#196981PurposeExpresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS2 mobility signal that confers graft mobility.DepositorInsertAtNIA1 gRNAs fused to TLS2 tags
UseTagsExpressionPlantMutationPromoterpU6-26, pU6-29Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
gRNA NIA TLS1
Plasmid#196980PurposeExpresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility.DepositorInsertAtNIA1 gRNAs fused to TLS1 tags
UseTagsExpressionPlantMutationPromoterpU6-26, pU6-29Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)
Plasmid#181792PurposeExpresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
UseTagsExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmirGLO-3'UTR-TNF
Plasmid#153069PurposeHuman TNF 3'UTR region cloned downstream of luciferase reporter geneDepositorInsert3' UTR region of TNF gene (TNF Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterPGKAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNCS-Antares
Plasmid#74279PurposeConstitutive expression of Antares in bacteriaDepositorInsertAntares
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA Flag ERbeta
Plasmid#35562DepositorInsertEstrogen Receptor beta (ESR2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSBbi 41BBL-CD86 mIL-15
Plasmid#183245PurposeExpression of human 41BBL, CD86, and membrane bound human IL-15 (IL-15-IL-15Ra) in a Sleeping Beauty transposon vector for use in artificial antigen presenting cellsDepositorInsert41BBL, CD86, mbIL-15 (IL-15-IL-15Ra)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadCBEd
Plasmid#193835PurposeExpress TadCBEd (with SpCas9) in mammalian cellsDepositorInsertTadA-CDd-SpCas9 D10A-2xUGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha1 P35S:LbCas12a:T35S (GB1441)
Plasmid#160611PurposeTranscriptional unit for the expression of the LbCas12a in plant systems driven by the CaMV35s promoter.DepositorInsertP35s:LbCas12a:T35s
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterP35SAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19-TdTwt
Plasmid#207466PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT)DepositorInsertTdTwt (Dntt Mouse)
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCH45 (multiAsCas12a-KRAB lenti)
Plasmid#217330PurposeLentiviral expression of multiAsCas12a-KRABDepositorInsertmultiAsCas12a
UseCRISPR and LentiviralTags6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2ExpressionMutationR1226A/E174R/S542R/K548RPromoterUCOE-SFFVAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Tet-OsTIR1(WT)-V5
Plasmid#158663PurposeTet-OsTIR1(WT)-V5 donor for integration at the AAVS1 locusDepositorInsertTet-OsTIR1(WT) AAVS1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE
Plasmid#138150PurposeFor cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInserthU6_Rfxcas13d-DR1_BsmBI
UseLentiviralTagsExpressionMutationDR1 A>TPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAY5 (mAID-EGFP-NLS piggyBac)
Plasmid#140532PurposemAID-EGFP-NLS piggyBacDepositorInsertmAID-EGFP-NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSBbi G418 Hu CD19
Plasmid#183247PurposeExpression of human CD19 antigen in a Sleeping Beauty transposon vectorDepositorInsertCD19 (CD19 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only
