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We narrowed to 14,582 results for: eng

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Showing: 4301 - 4320 of 14582 results

  1. pHAGE2-EF1a-E1MLS-2×FLAG-T4Lig-IRES-tdTomato

    Plasmid
    #234952
    Purpose
    Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid
    Depositor
    Agnel Sfeir
    Publication
    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.
    Insert
    human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    234952-plasmid-map-sequence-id-467733

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  2. AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-ecDHFR-enOsCas12f1-NLS-ecDHFR_pA_AAV2 ITR

    Plasmid
    #197029
    Purpose
    AAV vector encoding a human codon-optimized ecDHFR-enOsCas12f1-ecDHFR driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6
    Depositor
    Xiangfeng Kong
    Publication
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized ecDHFR-enOsCas12f1-ecDHFR
    Use
    AAV
    Mutation
    D52R / T132R
    Promoter
    MHCK7, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197029-plasmid-map-sequence-id-388970

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  6. pcDNA3-LYRa11-RBD-8h

    Plasmid
    #161822
    Purpose
    Mammalian expression plasmid for RBD of bat coronavirus isolate LYRa11
    Depositor
    Erik Procko
    Publication
    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf
    Insert
    Receptor-binding domain (RBD) of spike protein S
    Tags
    GSG linker and 8his tag and HA leader sequence
    Expression
    Mammalian
    Mutation
    Human codon optimized RBD (a.a. T324-D522)
    Promoter
    CMV
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    161822-plasmid-map-sequence-id-316758

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  7. pcDNA3-sACE2-T92Q-Fc(IgG1)

    Plasmid
    #145170
    Purpose
    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) glycosylation mutant T92Q
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Fc region of human IgG1
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    145170-plasmid-map-sequence-id-292630

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  10. pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)

    Plasmid
    #99229
    Purpose
    Mammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.
    Depositor
    Periklis Pantazis
    Publication
    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pr-mEos2
    Tags
    Lifeact
    Expression
    Mammalian
    Mutation
    A69T for primed conversion applications
    Promoter
    CMV
    Available since
    Oct. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    99229-plasmid-map-sequence-id-194794

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  12. Vector 1174D

    Plasmid
    #200012
    Purpose
    Fluorescent based sex separator in Aedes aegypti (SEPARATOR)
    Depositor
    Omar Akbari
    Publication
    Weng et al bioRxiv. 2023 Jun 16:2023.06.16.545348.
    Insert
    Engineered dobulesex splicing module
    Use
    Synthetic Biology
    Expression
    Insect
    Mutation
    Engineered dobulesex splicing module
    Promoter
    Hr5IE1
    Available since
    June 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    200012-plasmid-map-sequence-id-395199

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  13. pQE-BR1

    Plasmid
    #160333
    Purpose
    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is improved compared to wild type.
    Depositor
    Sang Taek Jung
    Publication
    Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12
    Insert
    BR1
    Tags
    6xHis tag
    Expression
    Bacterial
    Promoter
    T5
    Available since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    160333-plasmid-map-sequence-id-326604

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  14. pQE80L-SH3

    Plasmid
    #160331
    Purpose
    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its thermostability and quantum yield is improved compared to wild type.
    Depositor
    Sang Taek Jung
    Publication
    Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12
    Insert
    SH3
    Tags
    6xHis tag
    Expression
    Bacterial
    Promoter
    T5
    Available since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    160331-plasmid-map-sequence-id-326603

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  15. xCas12i

    Plasmid
    #195333
    Purpose
    vector for encoding a human codon-optimized xCas12i driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Publication
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon-optimized xCas12i
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CBh, CMV, hU6
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    195333-plasmid-map-sequence-id-383109

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  16. Cas12aUltra_5XNLS

    Plasmid
    #218775
    Purpose
    His-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLS
    Depositor
    Ross Wilson
    Publication
    Sahu et al Nat Protoc. 2025 Mar 3. doi: 10.1038/s41
    Insert
    5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a
    Use
    CRISPR
    Tags
    6XHis (C terminal), HA, Nucleoplasmin, SV40, and …
    Expression
    Bacterial
    Mutation
    Nucleotides mutations were made to encode for arg…
    Available since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    218775-plasmid-map-sequence-id-443448

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  18. pBiFC-PBZ-4A-VN

    Plasmid
    #110647
    Purpose
    expresses a PAR-binding (PBZ) domain fused to venus N-terminus, contains point mutations that abolish PAR binding
    Depositor
    Chris Lord
    Publication
    Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PBZ domain from APLF, amino acids 371-451 (APLF Human)
    Tags
    DDK and VN
    Expression
    Mammalian
    Mutation
    C379A, C385A, C421A and C427A
    Promoter
    CMV
    Available since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    110647-plasmid-map-sequence-id-217748

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  19. p-mCherry-C1-Phobos_CA

    Plasmid
    #98169
    Purpose
    slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression.
    Depositor
    Peter Hegemann
    Publication
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Synthetic construct Phobos_C128A gene
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, C128A, T15…
    Promoter
    CMV (+enhancer)
    Available since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98169-plasmid-map-sequence-id-182726

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  20. AAVS1-Puro CAG CLTX-CAR

    Plasmid
    #157743
    Purpose
    knock CLTX-CAR into AAVS1 safe harbor locus for constitutive expression
    Depositor
    Xiaoping Bao
    Publication
    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    chlorotoxin containing chimeric antigen receptor targeting glioblastoma
    Use
    CRISPR, Synthetic Biology, and TALEN
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    157743-plasmid-map-sequence-id-308780

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Showing: 4301 - 4320 of 14582 results