Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 7,059 results for: pcr

Clear keywords
Showing: 4301 - 4320 of 7059 results

  1. Cer(1-158)-gamma-12 in pcDNAI/Amp

    Plasmid
    #55629
    Purpose
    An N-terminal mCerulean fragment was fused to Ggamma-12. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    Cer(1-158)-gamma-12 (GNG12 Human, Aequorea victoria)
    Tags
    Cer(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-12 was amplified by PCR, which added a Bam…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55629-plasmid-map-sequence-id-115190

    Enlarge

  2. TC9pAB

    Plasmid
    #71310
    Purpose
    used as a template for in vitro transcription of Cas9 mRNA after linearizing with BbsI
    Depositor
    Karl Degenhardt
    Insert
    Cas9
    Use
    In vitro transcription
    Tags
    NLS
    Promoter
    T7
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    71310-plasmid-map-sequence-id-129410

    Enlarge

  3. (P79) OCN/Wnt 10b transgene

    Plasmid
    #25878
    Depositor
    Ormond MacDougald
    Insert
    Wnt10b (Wnt10b Mouse)
    Use
    Mouse Targeting
    Tags
    B-globin intron/poly A coding sequence and OCN pr…
    Expression
    Mammalian
    Available Since
    Oct. 21, 2010
    Availability
    Academic Institutions and Nonprofits only

  4. KPC2-pNic-Bsa4

    Plasmid
    #231082
    Purpose
    expresses Klebsiella pneumoniae carbapenemase-2 (KPC-2) under T7 with His tag
    Depositor
    Josh Beckham
    Insert
    carbapenemase-2 (kpc2 )
    Tags
    6x His tag
    Expression
    Bacterial
    Available Since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    231082-plasmid-map-sequence-id-462636

    Enlarge

  6. TC9nickpAB

    Plasmid
    #71311
    Purpose
    used as a template for in vitro transcription of Cas9 nickase mRNA after linearizing with BbsI
    Depositor
    Karl Degenhardt
    Insert
    Cas9 nickase
    Use
    In vitro transcription
    Tags
    NLS
    Mutation
    D10A nickase
    Promoter
    T7
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    71311-plasmid-map-sequence-id-131863

    Enlarge

  18. pQXCINeo-LOX

    Plasmid
    #134761
    Purpose
    a retroviral vector to express lysyl oxidase (derived from PCR4-TOPO-LOX)
    Depositor
    Philippe Clezardin, Martine Croset
    Insert
    LOX (LOX Human)
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    134761-plasmid-map-sequence-id-265190

    Enlarge

  19. Lmx1a-in situ probe

    Plasmid
    #45071
    Depositor
    Kathleen Millen
    Insert
    Lmx1a LIM homeobox transcription factor 1 alpha (residues 94-1149) (Lmx1a Mouse)
    Expression
    Mammalian
    Mutation
    Lmx1a residues 94-1149
    Available Since
    May 30, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 4301 - 4320 of 7059 results