We narrowed to 5,403 results for: puc
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Plasmid#188272PurposeMammalian expression, Genome editingDepositorInsertAaCas12b(Q119F/E475R/E758R)
UseCRISPRExpressionMammalianMutationnonePromoterCAGAvailable SinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA19-HSPB1 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)
Plasmid#199450PurposeCRISPR donor plasmid to insert TriTag (mTagBFP harbors 12XMS2 in its intron) into the C-terminus of human HSPB1 geneDepositorInsertHDR donor template
UseCRISPRAvailable SinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA22-SEC61B 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)
Plasmid#199453PurposeCRISPR donor plasmid to insert TriTag (mTagBFP) into the C-terminus of human SEC61B geneDepositorInsertHDR donor template
UseCRISPRAvailable SinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA20-HSPB1 5'HA-mTagBFP-3'-UTR 12XMS2V5-3'HA (HDR donor)
Plasmid#199451PurposeCRISPR donor plasmid to insert [mTagBFP-stop-12XMS2] into the C-terminus of human HSPB1 gene; 12XMS2 locates in the 3' UTR regionDepositorInsertHDR donor template
UseCRISPRAvailable SinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA21-HSPB1 5'HA-NarTag (12XPP7)-3'HA (HDR donor)
Plasmid#199452PurposeCRISPR donor plasmid to insert NarTag (mTagBFP harbors 12XPP7 in its intron) into the C-terminus of human HSPB1 geneDepositorInsertHDR donor template
UseCRISPRAvailable SinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcrVIB1-cloDF-amp
Plasmid#184843PurposeExpression of anti-CRISPR protein AcrVIB1 in bacteria.DepositorInsertanti-CRISPR protein inhibiting Cas13b nucleases
UseSynthetic BiologyExpressionBacterialPromoterJ23119Available SinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPG-GPD-TRPC
Plasmid#190113PurposeExpression vector for fungal (Aspergillus flavus) genes based on Aspergillus parasiticus pyrG gene selectionDepositorTypeEmpty backboneExpressionYeastAvailable SinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4D41
Plasmid#185841PurposeTesting the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3
ExpressionYeastMutationNoneAvailable SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIT7A6
Plasmid#185896PurposeIn vivo gene amplification (HapAmp) of nerolidol synthetic module at RPL25 locus (15 copy).DepositorInsertPRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PSk.GAL2>Y.FAST-EVBR1.2A>TRPL41B-PPDA1>RPL25(partial;Arm2)
ExpressionYeastMutationNoneAvailable SinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIALD2S
Plasmid#185865PurposeKnocking out ALD6 and expressing anacetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP ) in Saccharomyces cerevisiaeDepositorInsertALD6 (-125, 40)> PADH1> Lr.pduP> TPDC1-ALD6 (1054, 1749)
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF72 (B11)
Plasmid#185859PurposeTesting the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M3(2*PZ4)>(BamHI)yEGFP>TURA3
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP8EFA
Plasmid#185854PurposeTesting a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3
ExpressionYeastMutationDHFR P66LAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH616
Plasmid#169605PurposeTier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (PmPGK1-Fluc-pA::A2-pA::PCREm4-Nluc-pA).DepositorInsertPPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
ExpressionMammalianPromoterPCREm4 / PPGKAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2343_Tier3(SB)-YPet_Blast
Plasmid#169641PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-BlastR-pA-3'ITR)DepositorInsertPRPBSA-driven YPet and BlastR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2344_Tier3(SB)-mRuby2_Blast
Plasmid#169642PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a mRuby marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-BlastR-pA-3'ITR)DepositorInsertPRPBSA-driven mRuby2 and BlastR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2339_Tier3(SB)-Ruby_Puro
Plasmid#169637PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a mRuby2 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven mRuby2 and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2354_Tier3(PB)-Ruby_PuroR
Plasmid#169653PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a mRuby2 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven mRuby2 and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH614
Plasmid#169603PurposeTier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (PmPGK1-Fluc-pA::PCREm4-Nluc-pA::A3-pA).DepositorInsertPPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
ExpressionMammalianPromoterPCREm4 / PPGKAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH269-Tier1-OVanO2-PCMVmin2-SEAP-p2A-iRFP670
Plasmid#169667PurposeTier-1 vector encoding PVanO2-driven SEAP-p2A-iRFP670 expression (OVanO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).DepositorInsertvanillic acid-controlled SEAP and iRFP expression
ExpressionMammalianPromoterVanO2-PCMVminAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-RiboJ-mTFP1-T7
Plasmid#173225PurposeEncodes mTFP fluorescent protein. To be used in cell-free systemDepositorInsertmTFP1
UseCell-free systemExpressionBacterialPromoterT7Available SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only