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pLV-tetO-Tfap2a

Plasmid

#70274

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Tfap2a

Depositor

Hubert Schorle

Publication

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Tfap2a (Tfap2a Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223-CALM1

Plasmid

#23768

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CALM1 (CALM1 Human)

Use

Gateway Cloning

Mutation

M74T V144E

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pEX-CMV-SP-STIM2(15-746)

Plasmid

#18868

Depositor

Tobias Meyer

Publication

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Insert

STIM2 (STIM2 Human)

Expression

Mammalian

Mutation

Signal peptide(SP) 1-10 from STIM1 and residues 1…

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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plentiCMV-sp-HPV-TM(ICAM)

Plasmid

#196510

Purpose

Express HPV antigen epitope with ICAM transmembrane domain

Depositor

Howard Chang

Publication

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

a HPV epitope with CD8a stalk linker and ICAM TM domain

Use

Lentiviral

Tags

3xflag tag

Expression

Mammalian

Promoter

CMV

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-MCP-huADARE488QT490A

Plasmid

#159922

Purpose

Fusion of MS2 coat protein to Human ADAR2 E488QT490A, under Ef1a promoter, with WPRE. AKA 116v5

Depositor

Fei Chen

Publication

Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

Insert

Human ADAR2 E488QT490A (ADARB1 Human)

Use

AAV

Promoter

EF1a

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAd GFP HDAC5 WT

Plasmid

#32217

Depositor

Reuben Shaw

Publication

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

HDAC5 (HDAC5 Human)

Use

Adenoviral

Tags

GFP

Expression

Mammalian

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-BRD4

Plasmid

#23455

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BRD4 (BRD4 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
MyoD-pCLBabe

Plasmid

#20917

Depositor

Stephen Tapscott

Publication

Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

Insert

MyoD (Myod1 Mouse)

Use

Retroviral

Expression

Mammalian

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pKE4-I-SceI-fabp10a:Xpt-β-cat, cryaa:Venus

Plasmid

#105127

Purpose

Used along with I-SceI enzyme to generate transgenic zebrafish expressing hepatocyte-specific Xenopus activated beta-catenin and lens-specific Venus fluorescent protein

Depositor

Kimberley Evason, Didier Stainier

Publication

Evason et al PLoS Genet. 2015 Jul 2;11(7):e1005305. d

Inserts

Zebrafish fabp10a promoter:Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
Zebrafish cryaa promoter:Venus fluorescent protein coding sequence (cryaa Zebrafish)

Use

Zebrafish transgenesis

Promoter

cryaa (crystallin) and fabp10a

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ER50-SpCas9-ER50

Plasmid

#85448

Purpose

Expresses SpCas9 fused to ER50 domain on both N- and C-termini in mammalian cells

Depositor

Amit Choudhary

Publication

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

ER50-SpCas9-ER50

Use

AAV

Tags

destabilized domain of estrogen receptor ligand b…

Expression

Mammalian

Promoter

Cbh

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCXLE-dCas9VP192-T2A-EGFP-shP53

Plasmid

#69535

Purpose

Episomal plasmid encoding dCas9VP192 and p53 shRNA

Depositor

Timo Otonkoski

Publication

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

dCas9-dCas9VP192-GFP-shp53

Use

CRISPR

Tags

EGFP

Promoter

CAG

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b

Plasmid

#40731

Depositor

Daniel Gerlich

Publication

Wurzenberger et al J Cell Biol. 2012 Jul 23;198(2):173-83.

Insert

Aurora B FRET-sensor

Tags

CyPet and YPet

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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mEos4a-C1

Plasmid

#54810

Purpose

Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Publication

Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Type

Empty backbone

Tags

mEos4a

Expression

Mammalian

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pBCN22-R4R3

Plasmid

#26302

Depositor

Ben Lehner

Publication

Semple et al Nat Methods. 2010 Sep;7(9):725-7. doi: 1

Type

Empty backbone

Expression

Worm

Available since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only

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TU#710

Plasmid

#15278

Depositor

Martin Chalfie

Publication

Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

Insert

nzgfp

Expression

Worm

Available since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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pMAX deltaN-ERG

Plasmid

#29447

Depositor

Valeri Vasioukhin

Publication

Klezovitch et al Proc Natl Acad Sci U S A. 2008 Feb 12. 1

Insert

ERG (ERG Human)

Expression

Mammalian

Mutation

Deletion of first 33 aa of ERG

Available since

March 31, 2011

Availability

Academic Institutions and Nonprofits only
pME-puro-SNAP-FLAG-CD59

Plasmid

#50374

Purpose

Express SNAP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

Depositor

Reika Watanabe

Publication

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

SNAP

Expression

Mammalian

Promoter

SR alpha

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDONR223-STK24

Plasmid

#23595

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

STK24 (STK24 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pBabe-puro-miR-10b sponge

Plasmid

#25043

Depositor

Bob Weinberg

Publication

Ma et al Nat Biotechnol. 2010 Apr . 28(4):341-7.

Insert

miR-10b sponge

Use

Retroviral

Expression

Mammalian

Available since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pBS_nanorod_105b

Plasmid

#120347

Purpose

phagemid containing all components for assembly of a DNA origami nanorod (10-helix bundle)

Depositor

Hendrik Dietz

Publication

Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

Insert

Staple sequences for a DNA origami nanorod

Expression

Bacterial

Promoter

none

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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