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pC013 - Twinstrep-SUMO-huLwCas13a

Plasmid

#90097

Purpose

Twinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

Insert

LwCas13a

Use

CRISPR

Tags

6xHis-Twin Strep-SUMO

Expression

Bacterial

Available Since

June 13, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-hSFTPCpromoter(2kb)-EGFP-EF1a-TagRFP

Plasmid

#201681

Purpose

Lentiviral vector for human SFTPC promoter (2kb)-driven expression of EGFP and EF1a-driven TagRFP expression

Depositor

Emma Rawlins

Article

Lim et al bioRxiv [Preprint]. 2023 Sep 4:2023.08.3

Inserts

SFTPC promoter region (2kb upstream)
TagRFP

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-pHSE::Cre-mRuby3

Plasmid

#164136

Purpose

Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of heat induction

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Insert

HSE promoter-Cre-T2A-mRuby3

Use

CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

Expression

Mammalian

Promoter

HSE synthetic promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5/mCherry_pBirA-HA_FKBP8

Plasmid

#154457

Purpose

expresses mCherry_pBirA-HA_FKBP8/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

mCherry_pBirA-HA_FKBP8 (FKBP8 Human)

Expression

Mammalian

Promoter

CMV-F

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3/FKBP12-pBirA(79-C)-ha

Plasmid

#154449

Purpose

expresses FKBP12-pBirA(79-C)-ha/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

FKBP12-pBirA(79-C)-ha (FKBP12 Human)

Expression

Mammalian

Promoter

T7 promoter

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-monSTIM1

Plasmid

#161795

Purpose

Mammalian expression plasmid of monSTIM1 (ultra-photosensitive optogenetically-activated STIM1 protein)

Depositor

Won Do Heo

Article

Kim et al Nat Commun. 2020 Jan 10;11(1):210. doi:

Insert

MonSTIM1 (STIM1 Human, Mustard Weed)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgStk11#2/Cre

Plasmid

#173653

Purpose

Expresses a Stk11-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Stk11 (Stk11 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-G-AsCas12a crRNA-BsaI cassette (MSP3491)

Plasmid

#114067

Purpose

T7 promoter crRNA entry vector used for in vitro transcription of AsCas12a guides

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

entry vector for AsCas12a crRNAs

Use

In vitro transcription

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA™5/FRT/CMV_promoter-Cas9-E2A-mRFP

Plasmid

#164131

Purpose

Expresses SpCas9 and mRFP in mammalian cells (an insertion vector to generate Cas9-knockin cell lines)

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Insert

SpCas9 (cas9 Synthetic)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-GRET

Plasmid

#158222

Purpose

Control lentiviral plasmid encoding GFP-Nluc BRET reporter from Schaub et al., Cancer Research, 2015.

Depositor

Charles P. Lai

Article

Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

Insert

GFP-Nluc

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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